Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds

Abstract Liver disease cases are rapidly expanding across the globe and the only effective cure for end-stage disease is a transplant. Transplant procedures are costly and current supply of donor livers does not satisfy demand. Potential drug treatments and regenerative therapies that are being deve...

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Autores principales:	Thomas S. R. Bate, Victoria L. Gadd, Stuart J. Forbes, Anthony Callanan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29039efda635488b91aee81ad2c8b509
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spelling	oai:doaj.org-article:29039efda635488b91aee81ad2c8b5092021-12-02T14:06:32ZResponse differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds10.1038/s41598-021-81761-z2045-2322https://doaj.org/article/29039efda635488b91aee81ad2c8b5092021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81761-zhttps://doaj.org/toc/2045-2322Abstract Liver disease cases are rapidly expanding across the globe and the only effective cure for end-stage disease is a transplant. Transplant procedures are costly and current supply of donor livers does not satisfy demand. Potential drug treatments and regenerative therapies that are being developed to tackle these pressing issues require effective in-vitro culture platforms. Electrospun scaffolds provide bio-mimetic structures upon which cells are cultured to regulate function in-vitro. This study aims to shed light on the effects of electrospun PCL morphology on the culture of an immortalised hepatic cell line and mouse primary hepatocytes. Each cell type was cultured on large 4–5 µm fibres and small 1–2 µm fibres with random, aligned and highly porous cryogenically spun configurations. Cell attachment, proliferation, morphology and functional protein and gene expression was analysed. Results show that fibre morphology has a measurable influence on cellular morphology and function, with the alteration of key functional markers such as CYP1A2 expression.Thomas S. R. BateVictoria L. GaddStuart J. ForbesAnthony CallananNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas S. R. Bate Victoria L. Gadd Stuart J. Forbes Anthony Callanan Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds
description	Abstract Liver disease cases are rapidly expanding across the globe and the only effective cure for end-stage disease is a transplant. Transplant procedures are costly and current supply of donor livers does not satisfy demand. Potential drug treatments and regenerative therapies that are being developed to tackle these pressing issues require effective in-vitro culture platforms. Electrospun scaffolds provide bio-mimetic structures upon which cells are cultured to regulate function in-vitro. This study aims to shed light on the effects of electrospun PCL morphology on the culture of an immortalised hepatic cell line and mouse primary hepatocytes. Each cell type was cultured on large 4–5 µm fibres and small 1–2 µm fibres with random, aligned and highly porous cryogenically spun configurations. Cell attachment, proliferation, morphology and functional protein and gene expression was analysed. Results show that fibre morphology has a measurable influence on cellular morphology and function, with the alteration of key functional markers such as CYP1A2 expression.
format	article
author	Thomas S. R. Bate Victoria L. Gadd Stuart J. Forbes Anthony Callanan
author_facet	Thomas S. R. Bate Victoria L. Gadd Stuart J. Forbes Anthony Callanan
author_sort	Thomas S. R. Bate
title	Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds
title_short	Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds
title_full	Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds
title_fullStr	Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds
title_full_unstemmed	Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds
title_sort	response differences of hepg2 and primary mouse hepatocytes to morphological changes in electrospun pcl scaffolds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/29039efda635488b91aee81ad2c8b509
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Response differences of HepG2 and Primary Mouse Hepatocytes to morphological changes in electrospun PCL scaffolds

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