Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

Abstract In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest tha...

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Autores principales:	Bryon S. Donohoe, Hui Wei, Ashutosh Mittal, Todd Shollenberger, Vladimir V. Lunin, Michael E. Himmel, Roman Brunecky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/290c5e8d589d41a280b6d15cff5a0210
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spelling	oai:doaj.org-article:290c5e8d589d41a280b6d15cff5a02102021-12-02T16:06:39ZTowards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase10.1038/s41598-017-04502-12045-2322https://doaj.org/article/290c5e8d589d41a280b6d15cff5a02102017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04502-1https://doaj.org/toc/2045-2322Abstract In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.Bryon S. DonohoeHui WeiAshutosh MittalTodd ShollenbergerVladimir V. LuninMichael E. HimmelRoman BruneckyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bryon S. Donohoe Hui Wei Ashutosh Mittal Todd Shollenberger Vladimir V. Lunin Michael E. Himmel Roman Brunecky Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
description	Abstract In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.
format	article
author	Bryon S. Donohoe Hui Wei Ashutosh Mittal Todd Shollenberger Vladimir V. Lunin Michael E. Himmel Roman Brunecky
author_facet	Bryon S. Donohoe Hui Wei Ashutosh Mittal Todd Shollenberger Vladimir V. Lunin Michael E. Himmel Roman Brunecky
author_sort	Bryon S. Donohoe
title	Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_short	Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_full	Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_fullStr	Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_full_unstemmed	Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_sort	towards an understanding of enhanced biomass digestibility by in planta expression of a family 5 glycoside hydrolase
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/290c5e8d589d41a280b6d15cff5a0210
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Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

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