RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.

Optical imaging using multiphoton microscopy and whole body near infrared imaging has become a routine part of biomedical research. However, optical imaging methods rely on the availability of either small molecule reporters or genetically encoded fluorescent proteins, which are challenging and time...

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Autores principales:	Christian T Farrar, Christopher M William, Eloise Hudry, Tadafumi Hashimoto, Bradley T Hyman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29103be38c564781a83046c629cab3f0
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spelling	oai:doaj.org-article:29103be38c564781a83046c629cab3f02021-11-18T08:30:50ZRNA aptamer probes as optical imaging agents for the detection of amyloid plaques.1932-620310.1371/journal.pone.0089901https://doaj.org/article/29103be38c564781a83046c629cab3f02014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587111/?tool=EBIhttps://doaj.org/toc/1932-6203Optical imaging using multiphoton microscopy and whole body near infrared imaging has become a routine part of biomedical research. However, optical imaging methods rely on the availability of either small molecule reporters or genetically encoded fluorescent proteins, which are challenging and time consuming to develop. While directly labeled antibodies can also be used as imaging agents, antibodies are species specific, can typically not be tagged with multiple fluorescent reporters without interfering with target binding, and are bioactive, almost always eliciting a biological response and thereby influencing the process that is being studied. We examined the possibility of developing highly specific and sensitive optical imaging agents using aptamer technology. We developed a fluorescently tagged anti-Aβ RNA aptamer, β55, which binds amyloid plaques in both ex vivo human Alzheimer's disease brain tissue and in vivo APP/PS1 transgenic mice. Diffuse β55 positive halos, attributed to oligomeric Aβ, were observed surrounding the methoxy-XO4 positive plaque cores. Dot blots of synthetic Aβ aggregates provide further evidence that β55 binds both fibrillar and non-fibrillar Aβ. The high binding affinity, the ease of probe development, and the ability to incorporate multiple and multimodal imaging reporters suggest that RNA aptamers may have complementary and perhaps advantageous properties compared to conventional optical imaging probes and reporters.Christian T FarrarChristopher M WilliamEloise HudryTadafumi HashimotoBradley T HymanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89901 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian T Farrar Christopher M William Eloise Hudry Tadafumi Hashimoto Bradley T Hyman RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.
description	Optical imaging using multiphoton microscopy and whole body near infrared imaging has become a routine part of biomedical research. However, optical imaging methods rely on the availability of either small molecule reporters or genetically encoded fluorescent proteins, which are challenging and time consuming to develop. While directly labeled antibodies can also be used as imaging agents, antibodies are species specific, can typically not be tagged with multiple fluorescent reporters without interfering with target binding, and are bioactive, almost always eliciting a biological response and thereby influencing the process that is being studied. We examined the possibility of developing highly specific and sensitive optical imaging agents using aptamer technology. We developed a fluorescently tagged anti-Aβ RNA aptamer, β55, which binds amyloid plaques in both ex vivo human Alzheimer's disease brain tissue and in vivo APP/PS1 transgenic mice. Diffuse β55 positive halos, attributed to oligomeric Aβ, were observed surrounding the methoxy-XO4 positive plaque cores. Dot blots of synthetic Aβ aggregates provide further evidence that β55 binds both fibrillar and non-fibrillar Aβ. The high binding affinity, the ease of probe development, and the ability to incorporate multiple and multimodal imaging reporters suggest that RNA aptamers may have complementary and perhaps advantageous properties compared to conventional optical imaging probes and reporters.
format	article
author	Christian T Farrar Christopher M William Eloise Hudry Tadafumi Hashimoto Bradley T Hyman
author_facet	Christian T Farrar Christopher M William Eloise Hudry Tadafumi Hashimoto Bradley T Hyman
author_sort	Christian T Farrar
title	RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.
title_short	RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.
title_full	RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.
title_fullStr	RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.
title_full_unstemmed	RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.
title_sort	rna aptamer probes as optical imaging agents for the detection of amyloid plaques.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/29103be38c564781a83046c629cab3f0
work_keys_str_mv	AT christiantfarrar rnaaptamerprobesasopticalimagingagentsforthedetectionofamyloidplaques AT christophermwilliam rnaaptamerprobesasopticalimagingagentsforthedetectionofamyloidplaques AT eloisehudry rnaaptamerprobesasopticalimagingagentsforthedetectionofamyloidplaques AT tadafumihashimoto rnaaptamerprobesasopticalimagingagentsforthedetectionofamyloidplaques AT bradleythyman rnaaptamerprobesasopticalimagingagentsforthedetectionofamyloidplaques
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RNA aptamer probes as optical imaging agents for the detection of amyloid plaques.

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