Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury

Abstract Lipodystrophic mice are protected from cartilage damage following joint injury. This protection can be reversed by the implantation of a small adipose tissue graft. The purpose of this study was to evaluate the relationship between the gut microbiota and knee cartilage damage while controll...

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Autores principales:	Kelsey H. Collins, Drew J. Schwartz, Kristin L. Lenz, Charles A. Harris, Farshid Guilak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29153d110d634d57a3f3d4e6d8a19042
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spelling	oai:doaj.org-article:29153d110d634d57a3f3d4e6d8a190422021-12-02T16:14:09ZTaxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury10.1038/s41598-021-94125-42045-2322https://doaj.org/article/29153d110d634d57a3f3d4e6d8a190422021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94125-4https://doaj.org/toc/2045-2322Abstract Lipodystrophic mice are protected from cartilage damage following joint injury. This protection can be reversed by the implantation of a small adipose tissue graft. The purpose of this study was to evaluate the relationship between the gut microbiota and knee cartilage damage while controlling for adiposity, high fat diet, and joint injury using lipodystrophic (LD) mice. LD and littermate control (WT) mice were fed a high fat diet, chow diet, or were rescued with fat implantation, then challenged with destabilization of the medial meniscus surgery to induce osteoarthritis (OA). 16S rRNA sequencing was conducted on feces. MaAslin2 was used to determine associations between taxonomic relative abundance and OA severity. While serum LPS levels between groups were similar, synovial fluid LPS levels were increased in both limbs of HFD WT mice compared to all groups, except for fat transplanted animals. The Bacteroidetes:Firmicutes ratio of the gut microbiota was significantly reduced in HFD and OA-rescued animals when compared to chow. Nine novel significant associations were found between gut microbiota taxa and OA severity. These findings suggest the presence of causal relationships the gut microbiome and cartilage health, independent of diet or adiposity, providing potential therapeutic targets through manipulation of the microbiome.Kelsey H. CollinsDrew J. SchwartzKristin L. LenzCharles A. HarrisFarshid GuilakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kelsey H. Collins Drew J. Schwartz Kristin L. Lenz Charles A. Harris Farshid Guilak Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
description	Abstract Lipodystrophic mice are protected from cartilage damage following joint injury. This protection can be reversed by the implantation of a small adipose tissue graft. The purpose of this study was to evaluate the relationship between the gut microbiota and knee cartilage damage while controlling for adiposity, high fat diet, and joint injury using lipodystrophic (LD) mice. LD and littermate control (WT) mice were fed a high fat diet, chow diet, or were rescued with fat implantation, then challenged with destabilization of the medial meniscus surgery to induce osteoarthritis (OA). 16S rRNA sequencing was conducted on feces. MaAslin2 was used to determine associations between taxonomic relative abundance and OA severity. While serum LPS levels between groups were similar, synovial fluid LPS levels were increased in both limbs of HFD WT mice compared to all groups, except for fat transplanted animals. The Bacteroidetes:Firmicutes ratio of the gut microbiota was significantly reduced in HFD and OA-rescued animals when compared to chow. Nine novel significant associations were found between gut microbiota taxa and OA severity. These findings suggest the presence of causal relationships the gut microbiome and cartilage health, independent of diet or adiposity, providing potential therapeutic targets through manipulation of the microbiome.
format	article
author	Kelsey H. Collins Drew J. Schwartz Kristin L. Lenz Charles A. Harris Farshid Guilak
author_facet	Kelsey H. Collins Drew J. Schwartz Kristin L. Lenz Charles A. Harris Farshid Guilak
author_sort	Kelsey H. Collins
title	Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
title_short	Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
title_full	Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
title_fullStr	Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
title_full_unstemmed	Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
title_sort	taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/29153d110d634d57a3f3d4e6d8a19042
work_keys_str_mv	AT kelseyhcollins taxonomicchangesinthegutmicrobiotaareassociatedwithcartilagedamageindependentofadiposityhighfatdietandjointinjury AT drewjschwartz taxonomicchangesinthegutmicrobiotaareassociatedwithcartilagedamageindependentofadiposityhighfatdietandjointinjury AT kristinllenz taxonomicchangesinthegutmicrobiotaareassociatedwithcartilagedamageindependentofadiposityhighfatdietandjointinjury AT charlesaharris taxonomicchangesinthegutmicrobiotaareassociatedwithcartilagedamageindependentofadiposityhighfatdietandjointinjury AT farshidguilak taxonomicchangesinthegutmicrobiotaareassociatedwithcartilagedamageindependentofadiposityhighfatdietandjointinjury
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Taxonomic changes in the gut microbiota are associated with cartilage damage independent of adiposity, high fat diet, and joint injury

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