Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.

Gonadotropin-inhibitory hormone (GnIH) is a newly identified hypothalamic neuropeptide that inhibits pituitary hormone secretion in vertebrates. GnIH has an LPXRFamide (X = L or Q) motif at the C-terminal in representative species of gnathostomes. On the other hand, neuropeptide FF (NPFF), a neurope...

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Autores principales:	Tomohiro Osugi, Tomoki Okamura, You Lee Son, Makoto Ohkubo, Takayoshi Ubuka, Yasuhisa Henmi, Kazuyoshi Tsutsui
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2916077d4a7848e8ae7ecfc7cec6b2c2
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spelling	oai:doaj.org-article:2916077d4a7848e8ae7ecfc7cec6b2c22021-11-25T06:10:14ZEvolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.1932-620310.1371/journal.pone.0100962https://doaj.org/article/2916077d4a7848e8ae7ecfc7cec6b2c22014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24983238/?tool=EBIhttps://doaj.org/toc/1932-6203Gonadotropin-inhibitory hormone (GnIH) is a newly identified hypothalamic neuropeptide that inhibits pituitary hormone secretion in vertebrates. GnIH has an LPXRFamide (X = L or Q) motif at the C-terminal in representative species of gnathostomes. On the other hand, neuropeptide FF (NPFF), a neuropeptide characterized as a pain-modulatory neuropeptide, in vertebrates has a PQRFamide motif similar to the C-terminal of GnIH, suggesting that GnIH and NPFF have diverged from a common ancestor. Because GnIH and NPFF belong to the RFamide peptide family in vertebrates, protochordate RFamide peptides may provide important insights into the evolutionary origin of GnIH and NPFF. In this study, we identified a novel gene encoding RFamide peptides and two genes of their putative receptors in the amphioxus Branchiostoma japonicum. Molecular phylogenetic analysis and synteny analysis indicated that these genes are closely related to the genes of GnIH and NPFF and their receptors of vertebrates. We further identified mature RFamide peptides and their receptors in protochordates. The identified amphioxus RFamide peptides inhibited forskolin induced cAMP signaling in the COS-7 cells with one of the identified amphioxus RFamide peptide receptors expressed. These results indicate that the identified protochordate RFamide peptide gene is a common ancestral form of GnIH and NPFF genes, suggesting that the origin of GnIH and NPFF may date back to the time of the emergence of early chordates. GnIH gene and NPFF gene may have diverged by whole-genome duplication in the course of vertebrate evolution.Tomohiro OsugiTomoki OkamuraYou Lee SonMakoto OhkuboTakayoshi UbukaYasuhisa HenmiKazuyoshi TsutsuiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e100962 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomohiro Osugi Tomoki Okamura You Lee Son Makoto Ohkubo Takayoshi Ubuka Yasuhisa Henmi Kazuyoshi Tsutsui Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.
description	Gonadotropin-inhibitory hormone (GnIH) is a newly identified hypothalamic neuropeptide that inhibits pituitary hormone secretion in vertebrates. GnIH has an LPXRFamide (X = L or Q) motif at the C-terminal in representative species of gnathostomes. On the other hand, neuropeptide FF (NPFF), a neuropeptide characterized as a pain-modulatory neuropeptide, in vertebrates has a PQRFamide motif similar to the C-terminal of GnIH, suggesting that GnIH and NPFF have diverged from a common ancestor. Because GnIH and NPFF belong to the RFamide peptide family in vertebrates, protochordate RFamide peptides may provide important insights into the evolutionary origin of GnIH and NPFF. In this study, we identified a novel gene encoding RFamide peptides and two genes of their putative receptors in the amphioxus Branchiostoma japonicum. Molecular phylogenetic analysis and synteny analysis indicated that these genes are closely related to the genes of GnIH and NPFF and their receptors of vertebrates. We further identified mature RFamide peptides and their receptors in protochordates. The identified amphioxus RFamide peptides inhibited forskolin induced cAMP signaling in the COS-7 cells with one of the identified amphioxus RFamide peptide receptors expressed. These results indicate that the identified protochordate RFamide peptide gene is a common ancestral form of GnIH and NPFF genes, suggesting that the origin of GnIH and NPFF may date back to the time of the emergence of early chordates. GnIH gene and NPFF gene may have diverged by whole-genome duplication in the course of vertebrate evolution.
format	article
author	Tomohiro Osugi Tomoki Okamura You Lee Son Makoto Ohkubo Takayoshi Ubuka Yasuhisa Henmi Kazuyoshi Tsutsui
author_facet	Tomohiro Osugi Tomoki Okamura You Lee Son Makoto Ohkubo Takayoshi Ubuka Yasuhisa Henmi Kazuyoshi Tsutsui
author_sort	Tomohiro Osugi
title	Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.
title_short	Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.
title_full	Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.
title_fullStr	Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.
title_full_unstemmed	Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.
title_sort	evolutionary origin of gnih and npff in chordates: insights from novel amphioxus rfamide peptides.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2916077d4a7848e8ae7ecfc7cec6b2c2
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Evolutionary origin of GnIH and NPFF in chordates: insights from novel amphioxus RFamide peptides.

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