Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive fungal diseases of wheat worldwide. The cultivation and growth of resistant wheat varieties are the most economical, effective, and environmental friendly methods to control stripe rust. T...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pengfei Jin, Kaixiang Chao, Juan Li, Zihao Wang, Peng Cheng, Qiang Li, Baotong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	functional verification stripe rust resistance wheat-Leymus mollis YrM8664-3 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/29218162c07b433b9f46941640b3c775
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29218162c07b433b9f46941640b3c775
record_format	dspace
spelling	oai:doaj.org-article:29218162c07b433b9f46941640b3c7752021-11-19T16:32:00ZFunctional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-31664-462X10.3389/fpls.2021.754823https://doaj.org/article/29218162c07b433b9f46941640b3c7752021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.754823/fullhttps://doaj.org/toc/1664-462XStripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive fungal diseases of wheat worldwide. The cultivation and growth of resistant wheat varieties are the most economical, effective, and environmental friendly methods to control stripe rust. Therefore, it is necessary to use new resistance genes to breed resistant wheat varieties. A single dominant gene temporarily designated as YrM8664-3, from a wheat-Leymus mollis introgression line M8664-3 highly resistant to Chinese predominant Pst races, is a potentially valuable source of stripe rust resistance for breeding. Herein, based on previous YrM8664-3 chromosome location results (bin 4AL13-0.59-0.66 close to 4AL12-0.43-0.59) and expression change information of candidate genes and bioinformatics analysis, several candidate genes with significantly different expression changes were then selected and verified by virus-induced gene silencing (VIGS). Two of the candidate genes temporarily designated as TaFBN [containing plastid lipid-associated proteins (PAP)_fibrillin domain in its protein] and Ta_Pes_BRCT [containing Pescadillo and breast cancer tumour suppressor protein C-terminus (BRCT) domain in its protein], produced the most significant resistance changes in the wheat-Pst interaction system after silencing. These two genes were further verified by Agrobacterium-mediated wheat genetic transformation technology. According to the identification of disease resistance, the resistance function of the candidate gene TaFBN was further verified. Then, the expression of TaFBN under hormone treatment indicated that TaFBN may be related to the salicylic acid (SA) and abscisic acid (ABA) signaling pathways. Combined with the expression of TaFBN in response to environmental stress stimulation, it can be reasonably speculated that TaFBN plays an important role in the resistance of wheat to Pst and is involved in abiotic stress pathways.Pengfei JinKaixiang ChaoKaixiang ChaoJuan LiJuan LiZihao WangPeng ChengQiang LiBaotong WangFrontiers Media S.A.articlefunctional verificationstripe rustresistancewheat-Leymus mollisYrM8664-3Plant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	functional verification stripe rust resistance wheat-Leymus mollis YrM8664-3 Plant culture SB1-1110
spellingShingle	functional verification stripe rust resistance wheat-Leymus mollis YrM8664-3 Plant culture SB1-1110 Pengfei Jin Kaixiang Chao Kaixiang Chao Juan Li Juan Li Zihao Wang Peng Cheng Qiang Li Baotong Wang Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3
description	Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most widespread and destructive fungal diseases of wheat worldwide. The cultivation and growth of resistant wheat varieties are the most economical, effective, and environmental friendly methods to control stripe rust. Therefore, it is necessary to use new resistance genes to breed resistant wheat varieties. A single dominant gene temporarily designated as YrM8664-3, from a wheat-Leymus mollis introgression line M8664-3 highly resistant to Chinese predominant Pst races, is a potentially valuable source of stripe rust resistance for breeding. Herein, based on previous YrM8664-3 chromosome location results (bin 4AL13-0.59-0.66 close to 4AL12-0.43-0.59) and expression change information of candidate genes and bioinformatics analysis, several candidate genes with significantly different expression changes were then selected and verified by virus-induced gene silencing (VIGS). Two of the candidate genes temporarily designated as TaFBN [containing plastid lipid-associated proteins (PAP)_fibrillin domain in its protein] and Ta_Pes_BRCT [containing Pescadillo and breast cancer tumour suppressor protein C-terminus (BRCT) domain in its protein], produced the most significant resistance changes in the wheat-Pst interaction system after silencing. These two genes were further verified by Agrobacterium-mediated wheat genetic transformation technology. According to the identification of disease resistance, the resistance function of the candidate gene TaFBN was further verified. Then, the expression of TaFBN under hormone treatment indicated that TaFBN may be related to the salicylic acid (SA) and abscisic acid (ABA) signaling pathways. Combined with the expression of TaFBN in response to environmental stress stimulation, it can be reasonably speculated that TaFBN plays an important role in the resistance of wheat to Pst and is involved in abiotic stress pathways.
format	article
author	Pengfei Jin Kaixiang Chao Kaixiang Chao Juan Li Juan Li Zihao Wang Peng Cheng Qiang Li Baotong Wang
author_facet	Pengfei Jin Kaixiang Chao Kaixiang Chao Juan Li Juan Li Zihao Wang Peng Cheng Qiang Li Baotong Wang
author_sort	Pengfei Jin
title	Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3
title_short	Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3
title_full	Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3
title_fullStr	Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3
title_full_unstemmed	Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3
title_sort	functional verification of two genes related to stripe rust resistance in the wheat-leymus mollis introgression line m8664-3
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/29218162c07b433b9f46941640b3c775
work_keys_str_mv	AT pengfeijin functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT kaixiangchao functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT kaixiangchao functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT juanli functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT juanli functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT zihaowang functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT pengcheng functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT qiangli functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643 AT baotongwang functionalverificationoftwogenesrelatedtostriperustresistanceinthewheatleymusmollisintrogressionlinem86643
_version_	1718420001416806400

Functional Verification of Two Genes Related to Stripe Rust Resistance in the Wheat-Leymus mollis Introgression Line M8664-3

Ejemplares similares