Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves

The cell walls of forage chicory (Cichorium intybus) leaves are known to contain high proportions of pectic polysaccharides. However, little is known about the distribution of pectic polysaacharides among walls of different cell types/tissues and within walls. In this study, immunolabelling with fou...

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Autores principales:	Xuezhao Sun, Ian G. Andrew, Philip J. Harris, Simone O. Hoskin, Keith N. Joblin, Yuhua He
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	arabinan chicory galactan homogalacturonan immunolabelling pectins Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/2936c53e207e41a8afed40a1275bb22e
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spelling	oai:doaj.org-article:2936c53e207e41a8afed40a1275bb22e2021-11-22T07:28:40ZMapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves1664-462X10.3389/fpls.2021.762121https://doaj.org/article/2936c53e207e41a8afed40a1275bb22e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.762121/fullhttps://doaj.org/toc/1664-462XThe cell walls of forage chicory (Cichorium intybus) leaves are known to contain high proportions of pectic polysaccharides. However, little is known about the distribution of pectic polysaacharides among walls of different cell types/tissues and within walls. In this study, immunolabelling with four monoclonal antibodies was used to map the distribution of pectic polysaccharides in the cell walls of the laminae and midribs of these leaves. The antibodies JIM5 and JIM7 are specific for partially methyl-esterified homogalacturonans; LM5 and LM6 are specific for (1→4)-β-galactan and (1→5)-α-arabinan side chains, respectively, of rhamnogalacturonan I. All four antibodies labelled the walls of the epidermal cells with different intensities. JIM5 and JIM7, but not LM5 or LM6, labelled the middle lamella, tricellular junctions, and the corners of intercellular spaces of ground, xylem and phloem parenchyma. LM5, but not LM6, strongly labelled the walls of the few sclerenchyma fibres in the phloem of the midrib and lamina vascular bundles. The LM5 epitope was absent from some phloem parenchyma cells. LM6, but not LM5, strongly labelled the walls of the stomatal guard cells. The differential distribution of pectic epitopes among walls of different cell types and within walls may reflect the deposition and modification of these polysaccharides which are involved in cell wall properties and cell development.Xuezhao SunXuezhao SunIan G. AndrewPhilip J. HarrisSimone O. HoskinKeith N. JoblinYuhua HeYuhua HeFrontiers Media S.A.articlearabinanchicorygalactanhomogalacturonanimmunolabellingpectinsPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	arabinan chicory galactan homogalacturonan immunolabelling pectins Plant culture SB1-1110
spellingShingle	arabinan chicory galactan homogalacturonan immunolabelling pectins Plant culture SB1-1110 Xuezhao Sun Xuezhao Sun Ian G. Andrew Philip J. Harris Simone O. Hoskin Keith N. Joblin Yuhua He Yuhua He Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
description	The cell walls of forage chicory (Cichorium intybus) leaves are known to contain high proportions of pectic polysaccharides. However, little is known about the distribution of pectic polysaacharides among walls of different cell types/tissues and within walls. In this study, immunolabelling with four monoclonal antibodies was used to map the distribution of pectic polysaccharides in the cell walls of the laminae and midribs of these leaves. The antibodies JIM5 and JIM7 are specific for partially methyl-esterified homogalacturonans; LM5 and LM6 are specific for (1→4)-β-galactan and (1→5)-α-arabinan side chains, respectively, of rhamnogalacturonan I. All four antibodies labelled the walls of the epidermal cells with different intensities. JIM5 and JIM7, but not LM5 or LM6, labelled the middle lamella, tricellular junctions, and the corners of intercellular spaces of ground, xylem and phloem parenchyma. LM5, but not LM6, strongly labelled the walls of the few sclerenchyma fibres in the phloem of the midrib and lamina vascular bundles. The LM5 epitope was absent from some phloem parenchyma cells. LM6, but not LM5, strongly labelled the walls of the stomatal guard cells. The differential distribution of pectic epitopes among walls of different cell types and within walls may reflect the deposition and modification of these polysaccharides which are involved in cell wall properties and cell development.
format	article
author	Xuezhao Sun Xuezhao Sun Ian G. Andrew Philip J. Harris Simone O. Hoskin Keith N. Joblin Yuhua He Yuhua He
author_facet	Xuezhao Sun Xuezhao Sun Ian G. Andrew Philip J. Harris Simone O. Hoskin Keith N. Joblin Yuhua He Yuhua He
author_sort	Xuezhao Sun
title	Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
title_short	Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
title_full	Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
title_fullStr	Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
title_full_unstemmed	Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves
title_sort	mapping pectic-polysaccharide epitopes in cell walls of forage chicory (cichorium intybus) leaves
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2936c53e207e41a8afed40a1275bb22e
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Mapping Pectic-Polysaccharide Epitopes in Cell Walls of Forage Chicory (Cichorium intybus) Leaves

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