Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy

Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.

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Detalles Bibliográficos
Autores principales: Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/293de6b5849b489dbc388634cd7aeec1
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Sumario:Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.