Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves

The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the da...

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Autores principales:	Kirovski Danijela, Dodovski Petar, Savić Đorđe, Vujanac Ivan, Prodanović Radiša, Mirilović Milorad, Sladojević Željko, Djordjević Ana
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	cows placental deiodinases neonate thyroid axis ptu Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/2942839ebc2f4de9881ac9e655ac7b34
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spelling	oai:doaj.org-article:2942839ebc2f4de9881ac9e655ac7b342021-11-17T21:27:50ZPlacental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves1820-744810.1515/acve-2016-0005https://doaj.org/article/2942839ebc2f4de9881ac9e655ac7b342016-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0005https://doaj.org/toc/1820-7448The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life.Kirovski DanijelaDodovski PetarSavić ĐorđeVujanac IvanProdanović RadišaMirilović MiloradSladojević ŽeljkoDjordjević AnaSciendoarticlecowsplacental deiodinasesneonatethyroid axisptuVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 1, Pp 61-75 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	cows placental deiodinases neonate thyroid axis ptu Veterinary medicine SF600-1100
spellingShingle	cows placental deiodinases neonate thyroid axis ptu Veterinary medicine SF600-1100 Kirovski Danijela Dodovski Petar Savić Đorđe Vujanac Ivan Prodanović Radiša Mirilović Milorad Sladojević Željko Djordjević Ana Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves
description	The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life.
format	article
author	Kirovski Danijela Dodovski Petar Savić Đorđe Vujanac Ivan Prodanović Radiša Mirilović Milorad Sladojević Željko Djordjević Ana
author_facet	Kirovski Danijela Dodovski Petar Savić Đorđe Vujanac Ivan Prodanović Radiša Mirilović Milorad Sladojević Željko Djordjević Ana
author_sort	Kirovski Danijela
title	Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves
title_short	Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves
title_full	Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves
title_fullStr	Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves
title_full_unstemmed	Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves
title_sort	placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (ptu) and thyroid axis activity of their calves
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/2942839ebc2f4de9881ac9e655ac7b34
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Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves

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