Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels

Abstract The H. pylori outer inflammatory protein A (OipA) is an outer membrane protein that contributes to gastric inflammation. OipA is believed to affect intra-cellular signalling and modulate the host signalling pathways. The aim of the current study was to clarify the role of OipA in H. pylori...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Omid Teymournejad, Ashraf Mohabati Mobarez, Zuhair Mohammad Hassan, Amin Talebi Bezmin abadi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29479ec229bf4b78ac630d2b9288f9a5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29479ec229bf4b78ac630d2b9288f9a5
record_format	dspace
spelling	oai:doaj.org-article:29479ec229bf4b78ac630d2b9288f9a52021-12-02T12:32:46ZBinding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels10.1038/s41598-017-08176-72045-2322https://doaj.org/article/29479ec229bf4b78ac630d2b9288f9a52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08176-7https://doaj.org/toc/2045-2322Abstract The H. pylori outer inflammatory protein A (OipA) is an outer membrane protein that contributes to gastric inflammation. OipA is believed to affect intra-cellular signalling and modulate the host signalling pathways. The aim of the current study was to clarify the role of OipA in H. pylori pathogenesis and its effect on host cell signalling pathways. To this end, the oipA gene was isolated and inserted into cloning and expression vectors. The recombinant plasmid was transferred into an expression host to produce OipA, which was subsequently purified by affinity chromatography and used for antibody production. A confluent monolayer of gastric cell lines was treated with various concentrations of OipA and investigated for attachment, toxicity, and apoptosis and alterations in signalling pathways. OipA bound to gastric cell lines confirming its role in the attachment of H. pylori to host cells. The ratio of Bax/Bcl-2 and caspase3, 8, FasL in the host cells were assessed and the results showed that the Bax/Bcl-2 ratio as well as the level of cleaved-caspase 3 was elevated in OipA-treated cells. These findings suggest that OipA can bind and induce toxic events as well as triggering apoptotic cascade in host gastric cells through intrinsic pathway.Omid TeymournejadAshraf Mohabati MobarezZuhair Mohammad HassanAmin Talebi Bezmin abadiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Omid Teymournejad Ashraf Mohabati Mobarez Zuhair Mohammad Hassan Amin Talebi Bezmin abadi Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels
description	Abstract The H. pylori outer inflammatory protein A (OipA) is an outer membrane protein that contributes to gastric inflammation. OipA is believed to affect intra-cellular signalling and modulate the host signalling pathways. The aim of the current study was to clarify the role of OipA in H. pylori pathogenesis and its effect on host cell signalling pathways. To this end, the oipA gene was isolated and inserted into cloning and expression vectors. The recombinant plasmid was transferred into an expression host to produce OipA, which was subsequently purified by affinity chromatography and used for antibody production. A confluent monolayer of gastric cell lines was treated with various concentrations of OipA and investigated for attachment, toxicity, and apoptosis and alterations in signalling pathways. OipA bound to gastric cell lines confirming its role in the attachment of H. pylori to host cells. The ratio of Bax/Bcl-2 and caspase3, 8, FasL in the host cells were assessed and the results showed that the Bax/Bcl-2 ratio as well as the level of cleaved-caspase 3 was elevated in OipA-treated cells. These findings suggest that OipA can bind and induce toxic events as well as triggering apoptotic cascade in host gastric cells through intrinsic pathway.
format	article
author	Omid Teymournejad Ashraf Mohabati Mobarez Zuhair Mohammad Hassan Amin Talebi Bezmin abadi
author_facet	Omid Teymournejad Ashraf Mohabati Mobarez Zuhair Mohammad Hassan Amin Talebi Bezmin abadi
author_sort	Omid Teymournejad
title	Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels
title_short	Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels
title_full	Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels
title_fullStr	Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels
title_full_unstemmed	Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels
title_sort	binding of the helicobacter pylori oipa causes apoptosis of host cells via modulation of bax/bcl-2 levels
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/29479ec229bf4b78ac630d2b9288f9a5
work_keys_str_mv	AT omidteymournejad bindingofthehelicobacterpylorioipacausesapoptosisofhostcellsviamodulationofbaxbcl2levels AT ashrafmohabatimobarez bindingofthehelicobacterpylorioipacausesapoptosisofhostcellsviamodulationofbaxbcl2levels AT zuhairmohammadhassan bindingofthehelicobacterpylorioipacausesapoptosisofhostcellsviamodulationofbaxbcl2levels AT amintalebibezminabadi bindingofthehelicobacterpylorioipacausesapoptosisofhostcellsviamodulationofbaxbcl2levels
_version_	1718393967672819712

Binding of the Helicobacter pylori OipA causes apoptosis of host cells via modulation of Bax/Bcl-2 levels

Ejemplares similares