Effects of ambient particulate matter on a reconstructed human corneal epithelium model

Abstract We evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tiss...

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Autores principales:	Ryota Ko, Masahiko Hayashi, Miho Tanaka, Tomoaki Okuda, Chiharu Nishita-Hara, Hiroaki Ozaki, Eiichi Uchio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/294b4fb48b514d94986a3857cf971493
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spelling	oai:doaj.org-article:294b4fb48b514d94986a3857cf9714932021-12-02T14:26:55ZEffects of ambient particulate matter on a reconstructed human corneal epithelium model10.1038/s41598-021-82971-12045-2322https://doaj.org/article/294b4fb48b514d94986a3857cf9714932021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82971-1https://doaj.org/toc/2045-2322Abstract We evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tissues to PM concentrations of 1, 10, and 100 µg/mL for 24 h. After exposure, cell viability and interleukin (IL) IL-6 and IL-8 levels were determined, and haematoxylin and eosin and immunofluorescence staining of the zonula occludens-1 (ZO-1) were performed on tissue sections. In addition, the effects of a certified reference material of urban aerosols (UA; 100 µg/mL) were also examined as a reference. The viability of cells exposed to 100 μg/mL UA and PM>2.4 decreased to 76.2% ± 7.4 and 75.4% ± 16.1, respectively, whereas PM0.3–2.4 exposure had a limited effect on cell viability. These particles did not increase IL-6 and IL-8 levels significantly even though cell viability was decreased in 100 μg/mL UA and PM>2.4. ZO-1 expression was reduced in a dose-dependent manner in all groups. Reconstructed HCE could be used as an in vitro model to study the effects of environmental PM exposure on ocular surface cell viability and inflammation.Ryota KoMasahiko HayashiMiho TanakaTomoaki OkudaChiharu Nishita-HaraHiroaki OzakiEiichi UchioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryota Ko Masahiko Hayashi Miho Tanaka Tomoaki Okuda Chiharu Nishita-Hara Hiroaki Ozaki Eiichi Uchio Effects of ambient particulate matter on a reconstructed human corneal epithelium model
description	Abstract We evaluated the effects of ambient particulate matter (PM) on the corneal epithelium using a reconstructed human corneal epithelium (HCE) model. We collected two PM size fractions [aerodynamic diameter smaller than 2.4 µm: PM0.3–2.4 and larger than 2.4 µm: PM>2.4] and exposed these tissues to PM concentrations of 1, 10, and 100 µg/mL for 24 h. After exposure, cell viability and interleukin (IL) IL-6 and IL-8 levels were determined, and haematoxylin and eosin and immunofluorescence staining of the zonula occludens-1 (ZO-1) were performed on tissue sections. In addition, the effects of a certified reference material of urban aerosols (UA; 100 µg/mL) were also examined as a reference. The viability of cells exposed to 100 μg/mL UA and PM>2.4 decreased to 76.2% ± 7.4 and 75.4% ± 16.1, respectively, whereas PM0.3–2.4 exposure had a limited effect on cell viability. These particles did not increase IL-6 and IL-8 levels significantly even though cell viability was decreased in 100 μg/mL UA and PM>2.4. ZO-1 expression was reduced in a dose-dependent manner in all groups. Reconstructed HCE could be used as an in vitro model to study the effects of environmental PM exposure on ocular surface cell viability and inflammation.
format	article
author	Ryota Ko Masahiko Hayashi Miho Tanaka Tomoaki Okuda Chiharu Nishita-Hara Hiroaki Ozaki Eiichi Uchio
author_facet	Ryota Ko Masahiko Hayashi Miho Tanaka Tomoaki Okuda Chiharu Nishita-Hara Hiroaki Ozaki Eiichi Uchio
author_sort	Ryota Ko
title	Effects of ambient particulate matter on a reconstructed human corneal epithelium model
title_short	Effects of ambient particulate matter on a reconstructed human corneal epithelium model
title_full	Effects of ambient particulate matter on a reconstructed human corneal epithelium model
title_fullStr	Effects of ambient particulate matter on a reconstructed human corneal epithelium model
title_full_unstemmed	Effects of ambient particulate matter on a reconstructed human corneal epithelium model
title_sort	effects of ambient particulate matter on a reconstructed human corneal epithelium model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/294b4fb48b514d94986a3857cf971493
work_keys_str_mv	AT ryotako effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel AT masahikohayashi effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel AT mihotanaka effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel AT tomoakiokuda effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel AT chiharunishitahara effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel AT hiroakiozaki effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel AT eiichiuchio effectsofambientparticulatematteronareconstructedhumancornealepitheliummodel
_version_	1718391299090939904

Effects of ambient particulate matter on a reconstructed human corneal epithelium model

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