Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope

Abstract The extracellular egg-coating envelope that comprises a meshwork of filaments polymerized by glycoproteins plays a pivotal role in species-selective sperm recognition and subsequent fertilization; however, the structural and rheological properties conferring fertilization competence to the...

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Autores principales:	Mayu Hanaue, Naofumi Miwa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/294d020443e34c47b3d10458b077dd7c
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spelling	oai:doaj.org-article:294d020443e34c47b3d10458b077dd7c2021-12-02T15:06:23ZStructural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope10.1038/s41598-017-06093-32045-2322https://doaj.org/article/294d020443e34c47b3d10458b077dd7c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06093-3https://doaj.org/toc/2045-2322Abstract The extracellular egg-coating envelope that comprises a meshwork of filaments polymerized by glycoproteins plays a pivotal role in species-selective sperm recognition and subsequent fertilization; however, the structural and rheological properties conferring fertilization competence to the egg-coating envelope remain poorly unveiled. Here we show several nanoscale-structural and viscoelastic properties of the egg-coat using the transmission electron microscopy and the quartz crystal microbalance experiments, following clamp of the egg-coat at either fertilization-competent or -incompetent statuses by short-term pretreatment with synthetic peptides. Individual filament of approximately 4.8 nm diameter crossed one another, forming several types of intersections. Higher competence-inducing treatment changed the proportion of V-, Y-, and T-type intersections, and induced more randomly deflected angles at intersections. Incompetence-inducing treatment increased the median of a Gaussian distribution of filament lengths that had a peak of 10–20 nm under control conditions; furthermore, this treatment created bumps in the 30–40 and 50–60 nm windows. Quartz crystal microbalance study revealed that viscoelasticity of the competent VE suspension was lower than that of incompetent VE, indicating that viscoelastic property required for successful fertilization resides within a specific range. These findings indicated that the architecture of the egg-coat is capable of rapid and dynamic remodeling, which determines fertilization efficiency.Mayu HanaueNaofumi MiwaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mayu Hanaue Naofumi Miwa Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope
description	Abstract The extracellular egg-coating envelope that comprises a meshwork of filaments polymerized by glycoproteins plays a pivotal role in species-selective sperm recognition and subsequent fertilization; however, the structural and rheological properties conferring fertilization competence to the egg-coating envelope remain poorly unveiled. Here we show several nanoscale-structural and viscoelastic properties of the egg-coat using the transmission electron microscopy and the quartz crystal microbalance experiments, following clamp of the egg-coat at either fertilization-competent or -incompetent statuses by short-term pretreatment with synthetic peptides. Individual filament of approximately 4.8 nm diameter crossed one another, forming several types of intersections. Higher competence-inducing treatment changed the proportion of V-, Y-, and T-type intersections, and induced more randomly deflected angles at intersections. Incompetence-inducing treatment increased the median of a Gaussian distribution of filament lengths that had a peak of 10–20 nm under control conditions; furthermore, this treatment created bumps in the 30–40 and 50–60 nm windows. Quartz crystal microbalance study revealed that viscoelasticity of the competent VE suspension was lower than that of incompetent VE, indicating that viscoelastic property required for successful fertilization resides within a specific range. These findings indicated that the architecture of the egg-coat is capable of rapid and dynamic remodeling, which determines fertilization efficiency.
format	article
author	Mayu Hanaue Naofumi Miwa
author_facet	Mayu Hanaue Naofumi Miwa
author_sort	Mayu Hanaue
title	Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope
title_short	Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope
title_full	Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope
title_fullStr	Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope
title_full_unstemmed	Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope
title_sort	structural and rheological properties conferring fertilization competence to xenopus egg-coating envelope
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/294d020443e34c47b3d10458b077dd7c
work_keys_str_mv	AT mayuhanaue structuralandrheologicalpropertiesconferringfertilizationcompetencetoxenopuseggcoatingenvelope AT naofumimiwa structuralandrheologicalpropertiesconferringfertilizationcompetencetoxenopuseggcoatingenvelope
_version_	1718388438203367424

Structural and rheological properties conferring fertilization competence to Xenopus egg-coating envelope

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