Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection

Abstract A monkey model of Zika virus (ZIKV) infection is urgently needed to better understand transmission and pathogenesis, given its proven association with fetal brain defects in pregnant women and acute neurological illness. Here we experimentally infected 4 male marmosets with ZIKV (prototype...

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Autores principales:	Charles Y. Chiu, Claudia Sánchez-San Martín, Jerome Bouquet, Tony Li, Shigeo Yagi, Manasi Tamhankar, Vida L. Hodara, Laura M. Parodi, Sneha Somasekar, Guixia Yu, Luis D. Giavedoni, Suzette Tardif, Jean Patterson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/294e2f7fb0544b8eaa8d9c5fdf5f2b6d
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spelling	oai:doaj.org-article:294e2f7fb0544b8eaa8d9c5fdf5f2b6d2021-12-02T15:06:19ZExperimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection10.1038/s41598-017-17067-w2045-2322https://doaj.org/article/294e2f7fb0544b8eaa8d9c5fdf5f2b6d2017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17067-whttps://doaj.org/toc/2045-2322Abstract A monkey model of Zika virus (ZIKV) infection is urgently needed to better understand transmission and pathogenesis, given its proven association with fetal brain defects in pregnant women and acute neurological illness. Here we experimentally infected 4 male marmosets with ZIKV (prototype 1947 African strain) and monitored them clinically with sampling of various body fluids and tissues for nearly 3 months. We show that the course of acute infection with ZIKV in these New World monkeys resembles the human illness in many respects, including (1) lack of apparent clinical symptoms in most cases, (2) persistence of the virus in body fluids such as semen and saliva for longer periods of time than in serum, and (3) generation of neutralizing antibodies as well as an antiviral immunological host response. Importantly, ZIKV-infected saliva samples (in addition to serum) were found to be infectious, suggesting potential capacity for viral transmission by the oral route. Re-challenge of a previously infected marmoset with a contemporary outbreak strain SPH2015 from Brazil resulted in continued protection against infection, no viral shedding, and boosting of the immune response. Given the key similarities to human infection, a marmoset model of ZIKV infection may be useful for testing of new drugs and vaccines.Charles Y. ChiuClaudia Sánchez-San MartínJerome BouquetTony LiShigeo YagiManasi TamhankarVida L. HodaraLaura M. ParodiSneha SomasekarGuixia YuLuis D. GiavedoniSuzette TardifJean PattersonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charles Y. Chiu Claudia Sánchez-San Martín Jerome Bouquet Tony Li Shigeo Yagi Manasi Tamhankar Vida L. Hodara Laura M. Parodi Sneha Somasekar Guixia Yu Luis D. Giavedoni Suzette Tardif Jean Patterson Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
description	Abstract A monkey model of Zika virus (ZIKV) infection is urgently needed to better understand transmission and pathogenesis, given its proven association with fetal brain defects in pregnant women and acute neurological illness. Here we experimentally infected 4 male marmosets with ZIKV (prototype 1947 African strain) and monitored them clinically with sampling of various body fluids and tissues for nearly 3 months. We show that the course of acute infection with ZIKV in these New World monkeys resembles the human illness in many respects, including (1) lack of apparent clinical symptoms in most cases, (2) persistence of the virus in body fluids such as semen and saliva for longer periods of time than in serum, and (3) generation of neutralizing antibodies as well as an antiviral immunological host response. Importantly, ZIKV-infected saliva samples (in addition to serum) were found to be infectious, suggesting potential capacity for viral transmission by the oral route. Re-challenge of a previously infected marmoset with a contemporary outbreak strain SPH2015 from Brazil resulted in continued protection against infection, no viral shedding, and boosting of the immune response. Given the key similarities to human infection, a marmoset model of ZIKV infection may be useful for testing of new drugs and vaccines.
format	article
author	Charles Y. Chiu Claudia Sánchez-San Martín Jerome Bouquet Tony Li Shigeo Yagi Manasi Tamhankar Vida L. Hodara Laura M. Parodi Sneha Somasekar Guixia Yu Luis D. Giavedoni Suzette Tardif Jean Patterson
author_facet	Charles Y. Chiu Claudia Sánchez-San Martín Jerome Bouquet Tony Li Shigeo Yagi Manasi Tamhankar Vida L. Hodara Laura M. Parodi Sneha Somasekar Guixia Yu Luis D. Giavedoni Suzette Tardif Jean Patterson
author_sort	Charles Y. Chiu
title	Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
title_short	Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
title_full	Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
title_fullStr	Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
title_full_unstemmed	Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
title_sort	experimental zika virus inoculation in a new world monkey model reproduces key features of the human infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/294e2f7fb0544b8eaa8d9c5fdf5f2b6d
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Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection

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