Quantum dots affect expression of CD133 surface antigen in melanoma cells

Simona Steponkiene1-3, Simona Kavaliauskiene1, Rasa Purviniene4, Ricardas Rotomskis3,5, Petras Juzenas11Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Radiumhospital, Oslo, Norway; 2Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania; 3Biome...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Steponkiene S, Kavaliauskiene S, Purviniene R, Rotomskis R, Juzenas P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/29545772b3ff42e9ac30c3f79f1b51b6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29545772b3ff42e9ac30c3f79f1b51b6
record_format	dspace
spelling	oai:doaj.org-article:29545772b3ff42e9ac30c3f79f1b51b62021-12-02T05:08:46ZQuantum dots affect expression of CD133 surface antigen in melanoma cells1176-91141178-2013https://doaj.org/article/29545772b3ff42e9ac30c3f79f1b51b62011-10-01T00:00:00Zhttp://www.dovepress.com/quantum-dots-affect-expression-of-cd133-surface-antigen-in-melanoma-ce-a8521https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Simona Steponkiene1-3, Simona Kavaliauskiene1, Rasa Purviniene4, Ricardas Rotomskis3,5, Petras Juzenas11Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Radiumhospital, Oslo, Norway; 2Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania; 3Biomedical Physics Laboratory of Oncology Institute, Vilnius University, Vilnius, Lithuania; 4Immunology Laboratory of Oncology Institute, Vilnius University, Vilnius, Lithuania; 5Biophotonics Laboratory, Laser Research Center, Vilnius University, Vilnius, LithuaniaBackground: In novel treatment approaches, therapeutics should be designed to target cancer stem cells (CSCs). Quantum dots (QDs) are a promising new tool in fighting against cancer. However, little is known about accumulation and cytotoxicity of QDs in CSCs.Methods: Accumulation and cytotoxicity of CdTe-MPA (mercaptopropionic acid) QDs in CSCs were assessed using flow cytometry and fluorescence-activated cell sorting techniques as well as a colorimetric cell viability assay.Results: We investigated the expression of two cell surface-associated glycoproteins, CD44 and CD133, in four different cancer cell lines (glioblastoma, melanoma, pancreatic, and prostate adenocarcinoma). Only the melanoma cells were positive to both markers of CD44 and CD133, whereas the other cells were only CD44-positive. The QDs accumulated to a similar extent in all subpopulations of the melanoma cells. The phenotypical response after QD treatment was compared with the response after ionizing radiation treatment. The percentage of the CD44high-CD133high subpopulation decreased from 72% to 55%&ndash;58% for both treatments. The stem-like subpopulation CD44highCD133low/- increased from 26%&ndash;28% in the untreated melanoma cells to 36%&ndash;40% for both treatments.Conclusion: Treatment of melanoma cells with QDs results in an increase of stem-like cell subpopulations. The changes in phenotype distribution of the melanoma cells after the treatment with QDs are comparable with the changes after ionizing radiation.Keywords: prominin-1, CD44, glycoproteins, flow cytometry, FACS, nanoparticlesSteponkiene SKavaliauskiene SPurviniene RRotomskis RJuzenas PDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2437-2444 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Steponkiene S Kavaliauskiene S Purviniene R Rotomskis R Juzenas P Quantum dots affect expression of CD133 surface antigen in melanoma cells
description	Simona Steponkiene1-3, Simona Kavaliauskiene1, Rasa Purviniene4, Ricardas Rotomskis3,5, Petras Juzenas11Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Radiumhospital, Oslo, Norway; 2Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania; 3Biomedical Physics Laboratory of Oncology Institute, Vilnius University, Vilnius, Lithuania; 4Immunology Laboratory of Oncology Institute, Vilnius University, Vilnius, Lithuania; 5Biophotonics Laboratory, Laser Research Center, Vilnius University, Vilnius, LithuaniaBackground: In novel treatment approaches, therapeutics should be designed to target cancer stem cells (CSCs). Quantum dots (QDs) are a promising new tool in fighting against cancer. However, little is known about accumulation and cytotoxicity of QDs in CSCs.Methods: Accumulation and cytotoxicity of CdTe-MPA (mercaptopropionic acid) QDs in CSCs were assessed using flow cytometry and fluorescence-activated cell sorting techniques as well as a colorimetric cell viability assay.Results: We investigated the expression of two cell surface-associated glycoproteins, CD44 and CD133, in four different cancer cell lines (glioblastoma, melanoma, pancreatic, and prostate adenocarcinoma). Only the melanoma cells were positive to both markers of CD44 and CD133, whereas the other cells were only CD44-positive. The QDs accumulated to a similar extent in all subpopulations of the melanoma cells. The phenotypical response after QD treatment was compared with the response after ionizing radiation treatment. The percentage of the CD44high-CD133high subpopulation decreased from 72% to 55%&ndash;58% for both treatments. The stem-like subpopulation CD44highCD133low/- increased from 26%&ndash;28% in the untreated melanoma cells to 36%&ndash;40% for both treatments.Conclusion: Treatment of melanoma cells with QDs results in an increase of stem-like cell subpopulations. The changes in phenotype distribution of the melanoma cells after the treatment with QDs are comparable with the changes after ionizing radiation.Keywords: prominin-1, CD44, glycoproteins, flow cytometry, FACS, nanoparticles
format	article
author	Steponkiene S Kavaliauskiene S Purviniene R Rotomskis R Juzenas P
author_facet	Steponkiene S Kavaliauskiene S Purviniene R Rotomskis R Juzenas P
author_sort	Steponkiene S
title	Quantum dots affect expression of CD133 surface antigen in melanoma cells
title_short	Quantum dots affect expression of CD133 surface antigen in melanoma cells
title_full	Quantum dots affect expression of CD133 surface antigen in melanoma cells
title_fullStr	Quantum dots affect expression of CD133 surface antigen in melanoma cells
title_full_unstemmed	Quantum dots affect expression of CD133 surface antigen in melanoma cells
title_sort	quantum dots affect expression of cd133 surface antigen in melanoma cells
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/29545772b3ff42e9ac30c3f79f1b51b6
work_keys_str_mv	AT steponkienes quantumdotsaffectexpressionofcd133surfaceantigeninmelanomacells AT kavaliauskienes quantumdotsaffectexpressionofcd133surfaceantigeninmelanomacells AT purviniener quantumdotsaffectexpressionofcd133surfaceantigeninmelanomacells AT rotomskisr quantumdotsaffectexpressionofcd133surfaceantigeninmelanomacells AT juzenasp quantumdotsaffectexpressionofcd133surfaceantigeninmelanomacells
_version_	1718400580110516224

Quantum dots affect expression of CD133 surface antigen in melanoma cells

Ejemplares similares