Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum

Abstract WRKY transcription factors play important roles in plant growth development, resistance and substance metabolism regulation. However, the exact function of the response to salt stress in plants with specific WRKY transcription factors remains unclear. In this research, we isolated a new WRK...

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Autores principales:	Qian-yu Liang, Yin-huan Wu, Ke Wang, Zhen-yu Bai, Qing-lin Liu, Yuan-zhi Pan, Lei Zhang, Bei-bei Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2955b58f93cc40099eaa14a4923f4004
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spelling	oai:doaj.org-article:2955b58f93cc40099eaa14a4923f40042021-12-02T15:05:53ZChrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum10.1038/s41598-017-05170-x2045-2322https://doaj.org/article/2955b58f93cc40099eaa14a4923f40042017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05170-xhttps://doaj.org/toc/2045-2322Abstract WRKY transcription factors play important roles in plant growth development, resistance and substance metabolism regulation. However, the exact function of the response to salt stress in plants with specific WRKY transcription factors remains unclear. In this research, we isolated a new WRKY transcription factor DgWRKY5 from chrysanthemum. DgWRKY5 contains two WRKY domains of WKKYGQK and two C2H2 zinc fingers. The expression of DgWRKY5 in chrysanthemum was up-regulated under various treatments. Meanwhile, we observed higher expression levels in the leaves contrasted with other tissues. Under salt stress, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes in transgenic chrysanthemum were significantly higher than those in WT, whereas the accumulation of H2O2, O2 − and malondialdehyde (MDA) was reduced in transgenic chrysanthemum. Several parameters including root length, root length, fresh weight, chlorophyll content and leaf gas exchange parameters in transgenic chrysanthemum were much better compared with WT under salt stress. Moreover, the expression of stress-related genes DgAPX, DgCAT, DgNCED3A, DgNCED3B, DgCuZnSOD, DgP5CS, DgCSD1 and DgCSD2 was up-regulated in DgWRKY5 transgenic chrysanthemum compared with that in WT. These results suggested that DgWRKY5 could function as a positive regulator of salt stress in chrysanthemum.Qian-yu LiangYin-huan WuKe WangZhen-yu BaiQing-lin LiuYuan-zhi PanLei ZhangBei-bei JiangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qian-yu Liang Yin-huan Wu Ke Wang Zhen-yu Bai Qing-lin Liu Yuan-zhi Pan Lei Zhang Bei-bei Jiang Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum
description	Abstract WRKY transcription factors play important roles in plant growth development, resistance and substance metabolism regulation. However, the exact function of the response to salt stress in plants with specific WRKY transcription factors remains unclear. In this research, we isolated a new WRKY transcription factor DgWRKY5 from chrysanthemum. DgWRKY5 contains two WRKY domains of WKKYGQK and two C2H2 zinc fingers. The expression of DgWRKY5 in chrysanthemum was up-regulated under various treatments. Meanwhile, we observed higher expression levels in the leaves contrasted with other tissues. Under salt stress, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enzymes in transgenic chrysanthemum were significantly higher than those in WT, whereas the accumulation of H2O2, O2 − and malondialdehyde (MDA) was reduced in transgenic chrysanthemum. Several parameters including root length, root length, fresh weight, chlorophyll content and leaf gas exchange parameters in transgenic chrysanthemum were much better compared with WT under salt stress. Moreover, the expression of stress-related genes DgAPX, DgCAT, DgNCED3A, DgNCED3B, DgCuZnSOD, DgP5CS, DgCSD1 and DgCSD2 was up-regulated in DgWRKY5 transgenic chrysanthemum compared with that in WT. These results suggested that DgWRKY5 could function as a positive regulator of salt stress in chrysanthemum.
format	article
author	Qian-yu Liang Yin-huan Wu Ke Wang Zhen-yu Bai Qing-lin Liu Yuan-zhi Pan Lei Zhang Bei-bei Jiang
author_facet	Qian-yu Liang Yin-huan Wu Ke Wang Zhen-yu Bai Qing-lin Liu Yuan-zhi Pan Lei Zhang Bei-bei Jiang
author_sort	Qian-yu Liang
title	Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum
title_short	Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum
title_full	Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum
title_fullStr	Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum
title_full_unstemmed	Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum
title_sort	chrysanthemum wrky gene dgwrky5 enhances tolerance to salt stress in transgenic chrysanthemum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2955b58f93cc40099eaa14a4923f4004
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Chrysanthemum WRKY gene DgWRKY5 enhances tolerance to salt stress in transgenic chrysanthemum

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