Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments

Abstract Animal models are often used to assess the airborne transmissibility of various pathogens, which are typically assumed to be carried by expiratory droplets emitted directly from the respiratory tract of the infected animal. We recently established that influenza virus is also transmissible...

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Autores principales:	Sima Asadi, Manilyn J. Tupas, Ramya S. Barre, Anthony S. Wexler, Nicole M. Bouvier, William D. Ristenpart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2956797acfa1443cad6197da79c85596
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spelling	oai:doaj.org-article:2956797acfa1443cad6197da79c855962021-12-02T17:51:26ZNon-respiratory particles emitted by guinea pigs in airborne disease transmission experiments10.1038/s41598-021-96678-w2045-2322https://doaj.org/article/2956797acfa1443cad6197da79c855962021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96678-whttps://doaj.org/toc/2045-2322Abstract Animal models are often used to assess the airborne transmissibility of various pathogens, which are typically assumed to be carried by expiratory droplets emitted directly from the respiratory tract of the infected animal. We recently established that influenza virus is also transmissible via “aerosolized fomites,” micron-scale dust particulates released from virus-contaminated surfaces (Asadi et al. in Nat Commun 11(1):4062, 2020). Here we expand on this observation, by counting and characterizing the particles emitted from guinea pig cages using an Aerodynamic Particle Sizer (APS) and an Interferometric Mie Imaging (IMI) system. Of over 9000 airborne particles emitted from guinea pig cages and directly imaged with IMI, none had an interference pattern indicative of a liquid droplet. Separate measurements of the particle count using the APS indicate that particle concentrations spike upwards immediately following animal motion, then decay exponentially with a time constant commensurate with the air exchange rate in the cage. Taken together, the results presented here raise the possibility that a non-negligible fraction of airborne influenza transmission events between guinea pigs occurs via aerosolized fomites rather than respiratory droplets, though the relative frequencies of these two routes have yet to be definitively determined.Sima AsadiManilyn J. TupasRamya S. BarreAnthony S. WexlerNicole M. BouvierWilliam D. RistenpartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sima Asadi Manilyn J. Tupas Ramya S. Barre Anthony S. Wexler Nicole M. Bouvier William D. Ristenpart Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
description	Abstract Animal models are often used to assess the airborne transmissibility of various pathogens, which are typically assumed to be carried by expiratory droplets emitted directly from the respiratory tract of the infected animal. We recently established that influenza virus is also transmissible via “aerosolized fomites,” micron-scale dust particulates released from virus-contaminated surfaces (Asadi et al. in Nat Commun 11(1):4062, 2020). Here we expand on this observation, by counting and characterizing the particles emitted from guinea pig cages using an Aerodynamic Particle Sizer (APS) and an Interferometric Mie Imaging (IMI) system. Of over 9000 airborne particles emitted from guinea pig cages and directly imaged with IMI, none had an interference pattern indicative of a liquid droplet. Separate measurements of the particle count using the APS indicate that particle concentrations spike upwards immediately following animal motion, then decay exponentially with a time constant commensurate with the air exchange rate in the cage. Taken together, the results presented here raise the possibility that a non-negligible fraction of airborne influenza transmission events between guinea pigs occurs via aerosolized fomites rather than respiratory droplets, though the relative frequencies of these two routes have yet to be definitively determined.
format	article
author	Sima Asadi Manilyn J. Tupas Ramya S. Barre Anthony S. Wexler Nicole M. Bouvier William D. Ristenpart
author_facet	Sima Asadi Manilyn J. Tupas Ramya S. Barre Anthony S. Wexler Nicole M. Bouvier William D. Ristenpart
author_sort	Sima Asadi
title	Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
title_short	Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
title_full	Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
title_fullStr	Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
title_full_unstemmed	Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
title_sort	non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2956797acfa1443cad6197da79c85596
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Non-respiratory particles emitted by guinea pigs in airborne disease transmission experiments

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