Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles

Zhizeng Wang,1,* Qianqwen Zhou,1,* Yafei Guo,2,* Hangzhan Hu,1 Zhi Zheng,1 Shulian Li,1 Yaohui Wang,1 Yuanfang Ma1 1Joint National Laboratory for Antibody Drug Engineering, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, People’s Republic of China; 2School of Laborator...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang Z, Zhou Q, Guo Y, Hu H, Zheng Z, Li S, Wang Y, Ma Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	ractopamine salbutamol lateral flow selenium nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2965f17aa6014c72b504632c6147c241
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2965f17aa6014c72b504632c6147c241
record_format	dspace
spelling	oai:doaj.org-article:2965f17aa6014c72b504632c6147c2412021-12-02T13:26:20ZRapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles1178-2013https://doaj.org/article/2965f17aa6014c72b504632c6147c2412021-03-01T00:00:00Zhttps://www.dovepress.com/rapid-detection-of-ractopamine-and-salbutamol-in-swine-urine-by-immuno-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhizeng Wang,1,* Qianqwen Zhou,1,* Yafei Guo,2,* Hangzhan Hu,1 Zhi Zheng,1 Shulian Li,1 Yaohui Wang,1 Yuanfang Ma1 1Joint National Laboratory for Antibody Drug Engineering, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, People’s Republic of China; 2School of Laboratory, Sanquan college of Xinxiang Medical University, Xinxiang, Henan, 453003, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yaohui Wang; Yuanfang MaJoint National Laboratory for Antibody Drug Engineering, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, People’s Republic of ChinaTel +86 18237816739; +86 13803786139Email wangyaohui2001@sina.com; mayf@henu.edu.cnPurpose: The purpose of this study was to establish a lateral flow immunoassay using selenium nanoparticles (Se-NPs) as a probe to detect ractopamine (RAC) and salbutamol (SAL) in swine urine.Methods: SDS and PEG were used as templates to prepare Se-NPs; anti-RAC monoclonal antibodies or anti-SAL monoclonal antibodies were labelled with Se-NPs; and rapid detection kits were prepared. The sensitivity, specificity, and stability were measured, and actual samples were analysed.Results: The Se-NPs were spherical with a diameter of 40.63 ± 5.91 nm, and were conjugated successfully with an anti-RAC antibody to give a total diameter of 82.33 ± 17.91 nm. The detection limit of a RAC kit in swine urine was 1 ng/mL, and that of a SAL kit was 3 ng/mL. Both procedures could be completed within 5 minutes. No cross-reaction occurred with clenbuterol, bambuterol and phenylethanolamine A. Samples were tested consistently across different batches of kits for swine urine. The results of the kits were identical to those of actual clinical samples analysed by ELISA, and the coincidence rate was 100%.Conclusion: The assay kit does not require any special device for reading the results, and the readout is a simple colour change that can be evaluated with the naked eye. It is easy to operate, sensitive, specific, and stable This kit is suitable for the rapid and real-time detection of RAC and SAL residues in swine urine samples.Clinical Trial Registration: Swine urines samples were used under approval from the Experimental Animal Ethics committee of the Joint National Laboratory for Antibody Drug Engineering, Henan University.Keywords: ractopamine, salbutamol, lateral flow, selenium nanoparticlesWang ZZhou QGuo YHu HZheng ZLi SWang YMa YDove Medical Pressarticleractopaminesalbutamollateral flowselenium nanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 2059-2070 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ractopamine salbutamol lateral flow selenium nanoparticles Medicine (General) R5-920
spellingShingle	ractopamine salbutamol lateral flow selenium nanoparticles Medicine (General) R5-920 Wang Z Zhou Q Guo Y Hu H Zheng Z Li S Wang Y Ma Y Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles
description	Zhizeng Wang,1,* Qianqwen Zhou,1,* Yafei Guo,2,* Hangzhan Hu,1 Zhi Zheng,1 Shulian Li,1 Yaohui Wang,1 Yuanfang Ma1 1Joint National Laboratory for Antibody Drug Engineering, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, People’s Republic of China; 2School of Laboratory, Sanquan college of Xinxiang Medical University, Xinxiang, Henan, 453003, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yaohui Wang; Yuanfang MaJoint National Laboratory for Antibody Drug Engineering, School of Basic Medical Sciences, Henan University, Kaifeng, 475004, People’s Republic of ChinaTel +86 18237816739; +86 13803786139Email wangyaohui2001@sina.com; mayf@henu.edu.cnPurpose: The purpose of this study was to establish a lateral flow immunoassay using selenium nanoparticles (Se-NPs) as a probe to detect ractopamine (RAC) and salbutamol (SAL) in swine urine.Methods: SDS and PEG were used as templates to prepare Se-NPs; anti-RAC monoclonal antibodies or anti-SAL monoclonal antibodies were labelled with Se-NPs; and rapid detection kits were prepared. The sensitivity, specificity, and stability were measured, and actual samples were analysed.Results: The Se-NPs were spherical with a diameter of 40.63 ± 5.91 nm, and were conjugated successfully with an anti-RAC antibody to give a total diameter of 82.33 ± 17.91 nm. The detection limit of a RAC kit in swine urine was 1 ng/mL, and that of a SAL kit was 3 ng/mL. Both procedures could be completed within 5 minutes. No cross-reaction occurred with clenbuterol, bambuterol and phenylethanolamine A. Samples were tested consistently across different batches of kits for swine urine. The results of the kits were identical to those of actual clinical samples analysed by ELISA, and the coincidence rate was 100%.Conclusion: The assay kit does not require any special device for reading the results, and the readout is a simple colour change that can be evaluated with the naked eye. It is easy to operate, sensitive, specific, and stable This kit is suitable for the rapid and real-time detection of RAC and SAL residues in swine urine samples.Clinical Trial Registration: Swine urines samples were used under approval from the Experimental Animal Ethics committee of the Joint National Laboratory for Antibody Drug Engineering, Henan University.Keywords: ractopamine, salbutamol, lateral flow, selenium nanoparticles
format	article
author	Wang Z Zhou Q Guo Y Hu H Zheng Z Li S Wang Y Ma Y
author_facet	Wang Z Zhou Q Guo Y Hu H Zheng Z Li S Wang Y Ma Y
author_sort	Wang Z
title	Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles
title_short	Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles
title_full	Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles
title_fullStr	Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles
title_full_unstemmed	Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles
title_sort	rapid detection of ractopamine and salbutamol in swine urine by immunochromatography based on selenium nanoparticles
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/2965f17aa6014c72b504632c6147c241
work_keys_str_mv	AT wangz rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT zhouq rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT guoy rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT huh rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT zhengz rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT lis rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT wangy rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles AT may rapiddetectionofractopamineandsalbutamolinswineurinebyimmunochromatographybasedonseleniumnanoparticles
_version_	1718393019535720448

Rapid Detection of Ractopamine and Salbutamol in Swine Urine by Immunochromatography Based on Selenium Nanoparticles

Ejemplares similares