Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls

Abstract Endometriosis is a chronic, estrogen-dependent gynecological condition affecting approximately 10% of reproductive age women. The most widely accepted theory of its etiology includes retrograde menstruation. Recent reports suggest the uterus is not sterile. Thus, the refluxed menstrual effl...

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Autores principales:	Jocelyn M. Wessels, Miguel A. Domínguez, Nicholas A. Leyland, Sanjay K. Agarwal, Warren G. Foster
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/296d57970cb14bce911dfc671b0c72b9
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spelling	oai:doaj.org-article:296d57970cb14bce911dfc671b0c72b92021-12-02T17:26:55ZEndometrial microbiota is more diverse in people with endometriosis than symptomatic controls10.1038/s41598-021-98380-32045-2322https://doaj.org/article/296d57970cb14bce911dfc671b0c72b92021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98380-3https://doaj.org/toc/2045-2322Abstract Endometriosis is a chronic, estrogen-dependent gynecological condition affecting approximately 10% of reproductive age women. The most widely accepted theory of its etiology includes retrograde menstruation. Recent reports suggest the uterus is not sterile. Thus, the refluxed menstrual effluent may carry bacteria, and contribute to inflammation, the establishment and growth of endometriotic lesions. Here, we compared and contrasted uterine bacteria (endometrial microbiota) in people with surgically confirmed presence (N = 12) or absence of endometriosis (N = 9) using next-generation 16S rRNA gene sequencing. We obtained an average of > 9000 sequence reads per endometrial biopsy, and found the endometrial microbiota of people with endometriosis was more diverse (greater Shannon Diversity Index and proportion of ‘Other’ taxa) than symptomatic controls (with pelvic pain, surgically confirmed absence of endometriosis; diagnosed with other benign gynecological conditions). The relative abundance of bacterial taxa enriched in the endometrial microbiota of people with endometriosis belonged to the Actinobacteria phylum (Gram-positive), Oxalobacteraceae (Gram-negative) and Streptococcaceae (Gram-positive) families, and Tepidimonas (Gram-negative) genus, while those enriched in the symptomatic controls belonged to the Burkholderiaceae (Gram-negative) family, and Ralstonia (Gram-negative) genus. Taken together, results suggest the endometrial microbiota is perturbed in people with endometriosis.Jocelyn M. WesselsMiguel A. DomínguezNicholas A. LeylandSanjay K. AgarwalWarren G. FosterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jocelyn M. Wessels Miguel A. Domínguez Nicholas A. Leyland Sanjay K. Agarwal Warren G. Foster Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
description	Abstract Endometriosis is a chronic, estrogen-dependent gynecological condition affecting approximately 10% of reproductive age women. The most widely accepted theory of its etiology includes retrograde menstruation. Recent reports suggest the uterus is not sterile. Thus, the refluxed menstrual effluent may carry bacteria, and contribute to inflammation, the establishment and growth of endometriotic lesions. Here, we compared and contrasted uterine bacteria (endometrial microbiota) in people with surgically confirmed presence (N = 12) or absence of endometriosis (N = 9) using next-generation 16S rRNA gene sequencing. We obtained an average of > 9000 sequence reads per endometrial biopsy, and found the endometrial microbiota of people with endometriosis was more diverse (greater Shannon Diversity Index and proportion of ‘Other’ taxa) than symptomatic controls (with pelvic pain, surgically confirmed absence of endometriosis; diagnosed with other benign gynecological conditions). The relative abundance of bacterial taxa enriched in the endometrial microbiota of people with endometriosis belonged to the Actinobacteria phylum (Gram-positive), Oxalobacteraceae (Gram-negative) and Streptococcaceae (Gram-positive) families, and Tepidimonas (Gram-negative) genus, while those enriched in the symptomatic controls belonged to the Burkholderiaceae (Gram-negative) family, and Ralstonia (Gram-negative) genus. Taken together, results suggest the endometrial microbiota is perturbed in people with endometriosis.
format	article
author	Jocelyn M. Wessels Miguel A. Domínguez Nicholas A. Leyland Sanjay K. Agarwal Warren G. Foster
author_facet	Jocelyn M. Wessels Miguel A. Domínguez Nicholas A. Leyland Sanjay K. Agarwal Warren G. Foster
author_sort	Jocelyn M. Wessels
title	Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
title_short	Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
title_full	Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
title_fullStr	Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
title_full_unstemmed	Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
title_sort	endometrial microbiota is more diverse in people with endometriosis than symptomatic controls
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/296d57970cb14bce911dfc671b0c72b9
work_keys_str_mv	AT jocelynmwessels endometrialmicrobiotaismorediverseinpeoplewithendometriosisthansymptomaticcontrols AT migueladominguez endometrialmicrobiotaismorediverseinpeoplewithendometriosisthansymptomaticcontrols AT nicholasaleyland endometrialmicrobiotaismorediverseinpeoplewithendometriosisthansymptomaticcontrols AT sanjaykagarwal endometrialmicrobiotaismorediverseinpeoplewithendometriosisthansymptomaticcontrols AT warrengfoster endometrialmicrobiotaismorediverseinpeoplewithendometriosisthansymptomaticcontrols
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Endometrial microbiota is more diverse in people with endometriosis than symptomatic controls

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