Is gene transcription involved in seed dry after-ripening?

Orthodox seeds are living organisms that survive anhydrobiosis and may display dormancy, an inability to germinate at harvest. Seed germination potential can be acquired during a prolonged period of dry storage called after-ripening. The aim of this work was to determine if gene transcription is an...

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Autores principales:	Patrice Meimoun, Ernest Mordret, Nicolas B Langlade, Sandrine Balzergue, Sandrine Arribat, Christophe Bailly, Hayat El-Maarouf-Bouteau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/297f3507c3b84341a7f81f1c343ec490
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spelling	oai:doaj.org-article:297f3507c3b84341a7f81f1c343ec4902021-11-18T08:37:10ZIs gene transcription involved in seed dry after-ripening?1932-620310.1371/journal.pone.0086442https://doaj.org/article/297f3507c3b84341a7f81f1c343ec4902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466101/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Orthodox seeds are living organisms that survive anhydrobiosis and may display dormancy, an inability to germinate at harvest. Seed germination potential can be acquired during a prolonged period of dry storage called after-ripening. The aim of this work was to determine if gene transcription is an underlying regulatory mechanism for dormancy alleviation during after-ripening. To identify changes in gene transcription strictly associated with the acquisition of germination potential but not with storage, we used seed storage at low relative humidity that maintains dormancy as control. Transcriptome profiling was performed using DNA microarray to compare change in gene transcript abundance between dormant (D), after-ripened non-dormant (ND) and after-ripened dormant seeds (control, C). Quantitative real-time polymerase chain reaction (qPCR) was used to confirm gene expression. Comparison between D and ND showed the differential expression of 115 probesets at cut-off values of two-fold change (p<0.05). Comparisons between both D and C with ND in transcript abundance showed that only 13 transcripts, among 115, could be specific to dormancy alleviation. qPCR confirms the expression pattern of these transcripts but without significant variation between conditions. Here we show that sunflower seed dormancy alleviation in the dry state is not related to regulated changes in gene expression.Patrice MeimounErnest MordretNicolas B LangladeSandrine BalzergueSandrine ArribatChristophe BaillyHayat El-Maarouf-BouteauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86442 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrice Meimoun Ernest Mordret Nicolas B Langlade Sandrine Balzergue Sandrine Arribat Christophe Bailly Hayat El-Maarouf-Bouteau Is gene transcription involved in seed dry after-ripening?
description	Orthodox seeds are living organisms that survive anhydrobiosis and may display dormancy, an inability to germinate at harvest. Seed germination potential can be acquired during a prolonged period of dry storage called after-ripening. The aim of this work was to determine if gene transcription is an underlying regulatory mechanism for dormancy alleviation during after-ripening. To identify changes in gene transcription strictly associated with the acquisition of germination potential but not with storage, we used seed storage at low relative humidity that maintains dormancy as control. Transcriptome profiling was performed using DNA microarray to compare change in gene transcript abundance between dormant (D), after-ripened non-dormant (ND) and after-ripened dormant seeds (control, C). Quantitative real-time polymerase chain reaction (qPCR) was used to confirm gene expression. Comparison between D and ND showed the differential expression of 115 probesets at cut-off values of two-fold change (p<0.05). Comparisons between both D and C with ND in transcript abundance showed that only 13 transcripts, among 115, could be specific to dormancy alleviation. qPCR confirms the expression pattern of these transcripts but without significant variation between conditions. Here we show that sunflower seed dormancy alleviation in the dry state is not related to regulated changes in gene expression.
format	article
author	Patrice Meimoun Ernest Mordret Nicolas B Langlade Sandrine Balzergue Sandrine Arribat Christophe Bailly Hayat El-Maarouf-Bouteau
author_facet	Patrice Meimoun Ernest Mordret Nicolas B Langlade Sandrine Balzergue Sandrine Arribat Christophe Bailly Hayat El-Maarouf-Bouteau
author_sort	Patrice Meimoun
title	Is gene transcription involved in seed dry after-ripening?
title_short	Is gene transcription involved in seed dry after-ripening?
title_full	Is gene transcription involved in seed dry after-ripening?
title_fullStr	Is gene transcription involved in seed dry after-ripening?
title_full_unstemmed	Is gene transcription involved in seed dry after-ripening?
title_sort	is gene transcription involved in seed dry after-ripening?
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/297f3507c3b84341a7f81f1c343ec490
work_keys_str_mv	AT patricemeimoun isgenetranscriptioninvolvedinseeddryafterripening AT ernestmordret isgenetranscriptioninvolvedinseeddryafterripening AT nicolasblanglade isgenetranscriptioninvolvedinseeddryafterripening AT sandrinebalzergue isgenetranscriptioninvolvedinseeddryafterripening AT sandrinearribat isgenetranscriptioninvolvedinseeddryafterripening AT christophebailly isgenetranscriptioninvolvedinseeddryafterripening AT hayatelmaaroufbouteau isgenetranscriptioninvolvedinseeddryafterripening
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Is gene transcription involved in seed dry after-ripening?

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