Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins

Candida albicans-related bloodstream infections are often associated with infected central venous catheters (CVC) triggered by microbial adhesion and biofilm formation. We utilized single-cell force spectroscopy (SCFS) and flow chamber models to investigate the adhesion behavior of C. albicans yeast...

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Autores principales:	Philipp Jung, Clara E. Mischo, Gubesh Gunaratnam, Christian Spengler, Sören L. Becker, Bernhard Hube, Karin Jacobs, Markus Bischoff
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	candida albicans microbial adhesion germination als3 central venous catheters single-cell force spectroscopy Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/29813cac5aba4c1699bcc83c64e305d9
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spelling	oai:doaj.org-article:29813cac5aba4c1699bcc83c64e305d92021-11-17T14:21:59ZCandida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins2150-55942150-560810.1080/21505594.2020.1836902https://doaj.org/article/29813cac5aba4c1699bcc83c64e305d92020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1836902https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Candida albicans-related bloodstream infections are often associated with infected central venous catheters (CVC) triggered by microbial adhesion and biofilm formation. We utilized single-cell force spectroscopy (SCFS) and flow chamber models to investigate the adhesion behavior of C. albicans yeast cells and germinated cells to naïve and human blood plasma (HBP)-coated CVC tubing. Germinated cells demonstrated up to 56.8-fold increased adhesion forces to CVC surfaces when compared to yeast cells. Coating of CVCs with HBP significantly increased the adhesion of 60-min germinated cells but not of yeast cells and 30-min germinated cells. Under flow conditions comparable to those in major human veins, germinated cells displayed a flow directional-orientated adhesion pattern to HBP-coated CVC material, suggesting the germ tip to serve as the major adhesive region. None of the above-reported phenotypes were observed with germinated cells of an als3Δ deletion mutant, which displayed similar adhesion forces to CVC surfaces as the isogenic yeast cells. Germinated cells of the als3Δ mutant also lacked a clear flow directional-orientated adhesion pattern on HBP-coated CVC material, indicating a central role for Als3 in the adhesion of germinated C. albicans cells to blood exposed CVC surfaces. In the common model of C. albicans, biofilm formation is thought to be mediated primarily by yeast cells, followed by surface-triggered the formation of hyphae. We suggest an extension of this model in which C. albicans germ tubes promote the initial adhesion to blood-exposed implanted medical devices via the germ tube-associated adhesion protein Als3.Philipp JungClara E. MischoGubesh GunaratnamChristian SpenglerSören L. BeckerBernhard HubeKarin JacobsMarkus BischoffTaylor & Francis Grouparticlecandida albicansmicrobial adhesiongerminationals3central venous catheterssingle-cell force spectroscopyInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1453-1465 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	candida albicans microbial adhesion germination als3 central venous catheters single-cell force spectroscopy Infectious and parasitic diseases RC109-216
spellingShingle	candida albicans microbial adhesion germination als3 central venous catheters single-cell force spectroscopy Infectious and parasitic diseases RC109-216 Philipp Jung Clara E. Mischo Gubesh Gunaratnam Christian Spengler Sören L. Becker Bernhard Hube Karin Jacobs Markus Bischoff Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
description	Candida albicans-related bloodstream infections are often associated with infected central venous catheters (CVC) triggered by microbial adhesion and biofilm formation. We utilized single-cell force spectroscopy (SCFS) and flow chamber models to investigate the adhesion behavior of C. albicans yeast cells and germinated cells to naïve and human blood plasma (HBP)-coated CVC tubing. Germinated cells demonstrated up to 56.8-fold increased adhesion forces to CVC surfaces when compared to yeast cells. Coating of CVCs with HBP significantly increased the adhesion of 60-min germinated cells but not of yeast cells and 30-min germinated cells. Under flow conditions comparable to those in major human veins, germinated cells displayed a flow directional-orientated adhesion pattern to HBP-coated CVC material, suggesting the germ tip to serve as the major adhesive region. None of the above-reported phenotypes were observed with germinated cells of an als3Δ deletion mutant, which displayed similar adhesion forces to CVC surfaces as the isogenic yeast cells. Germinated cells of the als3Δ mutant also lacked a clear flow directional-orientated adhesion pattern on HBP-coated CVC material, indicating a central role for Als3 in the adhesion of germinated C. albicans cells to blood exposed CVC surfaces. In the common model of C. albicans, biofilm formation is thought to be mediated primarily by yeast cells, followed by surface-triggered the formation of hyphae. We suggest an extension of this model in which C. albicans germ tubes promote the initial adhesion to blood-exposed implanted medical devices via the germ tube-associated adhesion protein Als3.
format	article
author	Philipp Jung Clara E. Mischo Gubesh Gunaratnam Christian Spengler Sören L. Becker Bernhard Hube Karin Jacobs Markus Bischoff
author_facet	Philipp Jung Clara E. Mischo Gubesh Gunaratnam Christian Spengler Sören L. Becker Bernhard Hube Karin Jacobs Markus Bischoff
author_sort	Philipp Jung
title	Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
title_short	Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
title_full	Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
title_fullStr	Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
title_full_unstemmed	Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
title_sort	candida albicans adhesion to central venous catheters: impact of blood plasma-driven germ tube formation and pathogen-derived adhesins
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/29813cac5aba4c1699bcc83c64e305d9
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Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins

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