Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths

The synthesis of ultra-small gold nanoclusters (Au NCs) with sizes down to 2 nm has received increasing interest due to their unique optical and electronic properties. Like many peptide-coated gold nanospheres synthesized before, modified gold nanoclusters with peptide conjugation are potentially si...

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Autores principales:	Qun Ma, Lichao Liu, Zeyue Yang, Peng Zheng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gold nanocluster peptide conjugation elastin-like polypeptide single-molecule force spectroscopy Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2985bb62720248e4b7decc5d38d7325b
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spelling	oai:doaj.org-article:2985bb62720248e4b7decc5d38d7325b2021-11-25T18:30:57ZFacile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths10.3390/nano111129322079-4991https://doaj.org/article/2985bb62720248e4b7decc5d38d7325b2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2932https://doaj.org/toc/2079-4991The synthesis of ultra-small gold nanoclusters (Au NCs) with sizes down to 2 nm has received increasing interest due to their unique optical and electronic properties. Like many peptide-coated gold nanospheres synthesized before, modified gold nanoclusters with peptide conjugation are potentially significant in biomedical and catalytic fields. Here, we explore whether such small-sized gold nanoclusters can be conjugated with peptides also and characterize them using atomic force microscopy. Using a long and flexible elastin-like polypeptide (ELP)<sub>20</sub> as the conjugated peptide, (ELP)<sub>20</sub>-Au NCs was successfully synthesized via a one-pot synthesis method. The unique optical and electronic properties of gold nanoclusters are still preserved, while a much larger size was obtained as expected due to the peptide conjugation. In addition, a short and rigid peptide (EAAAK)<sub>3</sub> was conjugated to the gold nanoclusters. Their Yong’s modulus was characterized using atomic force microscopy (AFM). Moreover, the coated peptide on the nanoclusters was pulled using AFM-based single molecule-force spectroscopy (SMFS), showing expected properties as one of the first force spectroscopy experiments on peptide-coated nanoclusters. Our results pave the way for further modification of nanoclusters based on the conjugated peptides and show a new method to characterize these materials using AFM-SMFS.Qun MaLichao LiuZeyue YangPeng ZhengMDPI AGarticlegold nanoclusterpeptide conjugationelastin-like polypeptidesingle-molecule force spectroscopyChemistryQD1-999ENNanomaterials, Vol 11, Iss 2932, p 2932 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanocluster peptide conjugation elastin-like polypeptide single-molecule force spectroscopy Chemistry QD1-999
spellingShingle	gold nanocluster peptide conjugation elastin-like polypeptide single-molecule force spectroscopy Chemistry QD1-999 Qun Ma Lichao Liu Zeyue Yang Peng Zheng Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths
description	The synthesis of ultra-small gold nanoclusters (Au NCs) with sizes down to 2 nm has received increasing interest due to their unique optical and electronic properties. Like many peptide-coated gold nanospheres synthesized before, modified gold nanoclusters with peptide conjugation are potentially significant in biomedical and catalytic fields. Here, we explore whether such small-sized gold nanoclusters can be conjugated with peptides also and characterize them using atomic force microscopy. Using a long and flexible elastin-like polypeptide (ELP)<sub>20</sub> as the conjugated peptide, (ELP)<sub>20</sub>-Au NCs was successfully synthesized via a one-pot synthesis method. The unique optical and electronic properties of gold nanoclusters are still preserved, while a much larger size was obtained as expected due to the peptide conjugation. In addition, a short and rigid peptide (EAAAK)<sub>3</sub> was conjugated to the gold nanoclusters. Their Yong’s modulus was characterized using atomic force microscopy (AFM). Moreover, the coated peptide on the nanoclusters was pulled using AFM-based single molecule-force spectroscopy (SMFS), showing expected properties as one of the first force spectroscopy experiments on peptide-coated nanoclusters. Our results pave the way for further modification of nanoclusters based on the conjugated peptides and show a new method to characterize these materials using AFM-SMFS.
format	article
author	Qun Ma Lichao Liu Zeyue Yang Peng Zheng
author_facet	Qun Ma Lichao Liu Zeyue Yang Peng Zheng
author_sort	Qun Ma
title	Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths
title_short	Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths
title_full	Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths
title_fullStr	Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths
title_full_unstemmed	Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths
title_sort	facile synthesis of peptide-conjugated gold nanoclusters with different lengths
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2985bb62720248e4b7decc5d38d7325b
work_keys_str_mv	AT qunma facilesynthesisofpeptideconjugatedgoldnanoclusterswithdifferentlengths AT lichaoliu facilesynthesisofpeptideconjugatedgoldnanoclusterswithdifferentlengths AT zeyueyang facilesynthesisofpeptideconjugatedgoldnanoclusterswithdifferentlengths AT pengzheng facilesynthesisofpeptideconjugatedgoldnanoclusterswithdifferentlengths
_version_	1718411004240461824

Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths

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