Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada

The role of the accompanying microbiota in the presence of <i>Listeria monocytogenes</i> on meat processing surfaces is not yet understood, especially in industrial production conditions. In this study, 300 conveyor belt samples from the cutting room of a swine slaughterhouse were collec...

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Autores principales:	Fanie Shedleur-Bourguignon, William P. Thériault, Jessie Longpré, Alexandre Thibodeau, Philippe Fravalo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Listeria monocytogenes</i> accompanying microbiota conveyor belt surfaces heterogeneous spatial contamination <i>Veillonella</i> Medicine R
Acceso en línea:	https://doaj.org/article/2987ebd4aa1244a68807c9c9d5ccd422
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spelling	oai:doaj.org-article:2987ebd4aa1244a68807c9c9d5ccd4222021-11-25T18:37:41ZUse of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada10.3390/pathogens101113682076-0817https://doaj.org/article/2987ebd4aa1244a68807c9c9d5ccd4222021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1368https://doaj.org/toc/2076-0817The role of the accompanying microbiota in the presence of <i>Listeria monocytogenes</i> on meat processing surfaces is not yet understood, especially in industrial production conditions. In this study, 300 conveyor belt samples from the cutting room of a swine slaughterhouse were collected during production. The samples were subjected to the detection of <i>L. monocytogenes</i>. Recovered strains were characterized by serogrouping-PCR, InlA Sanger sequencing and for their ability to form biofilm. A selection of isolates was compared with core genome multi-locus sequence typing analysis (cgMLST). The sequencing of the V4 region of the 16S RNA gene of the microorganisms harvested from each sample was carried out in parallel using the Illumina MiSeq platform. Diversity analyses were performed and MaAsLin analysis was used to assess the link between <i>L. monocytogenes</i> detection and the surrounding bacteria. The 72 isolates collected showed a low genetic diversity and important persistence characteristics. <i>L. monocytogenes</i> isolates were not stochastically distributed on the surfaces: the isolates were detected on three out of six production lines, each associated with a specific meat cut: the half carcasses, the bostons and the picnics. MaAsLin biomarker analysis identified the taxa <i>Veillonella</i> (<i>p</i> ≤ 0.0397) as a bacterial determinant of the presence of <i>L. monocytogenes</i> on processing surfaces. The results of this study revealed a heterogenous contamination pattern of the processing surfaces by <i>L. monocytogenes</i> and targeted a bacterial indicator of the presence of the pathogen. These results could lead to a better risk assessment of the contamination of meat products.Fanie Shedleur-BourguignonWilliam P. ThériaultJessie LongpréAlexandre ThibodeauPhilippe FravaloMDPI AGarticle<i>Listeria monocytogenes</i>accompanying microbiotaconveyor belt surfacesheterogeneous spatial contamination<i>Veillonella</i>MedicineRENPathogens, Vol 10, Iss 1368, p 1368 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Listeria monocytogenes</i> accompanying microbiota conveyor belt surfaces heterogeneous spatial contamination <i>Veillonella</i> Medicine R
spellingShingle	<i>Listeria monocytogenes</i> accompanying microbiota conveyor belt surfaces heterogeneous spatial contamination <i>Veillonella</i> Medicine R Fanie Shedleur-Bourguignon William P. Thériault Jessie Longpré Alexandre Thibodeau Philippe Fravalo Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
description	The role of the accompanying microbiota in the presence of <i>Listeria monocytogenes</i> on meat processing surfaces is not yet understood, especially in industrial production conditions. In this study, 300 conveyor belt samples from the cutting room of a swine slaughterhouse were collected during production. The samples were subjected to the detection of <i>L. monocytogenes</i>. Recovered strains were characterized by serogrouping-PCR, InlA Sanger sequencing and for their ability to form biofilm. A selection of isolates was compared with core genome multi-locus sequence typing analysis (cgMLST). The sequencing of the V4 region of the 16S RNA gene of the microorganisms harvested from each sample was carried out in parallel using the Illumina MiSeq platform. Diversity analyses were performed and MaAsLin analysis was used to assess the link between <i>L. monocytogenes</i> detection and the surrounding bacteria. The 72 isolates collected showed a low genetic diversity and important persistence characteristics. <i>L. monocytogenes</i> isolates were not stochastically distributed on the surfaces: the isolates were detected on three out of six production lines, each associated with a specific meat cut: the half carcasses, the bostons and the picnics. MaAsLin biomarker analysis identified the taxa <i>Veillonella</i> (<i>p</i> ≤ 0.0397) as a bacterial determinant of the presence of <i>L. monocytogenes</i> on processing surfaces. The results of this study revealed a heterogenous contamination pattern of the processing surfaces by <i>L. monocytogenes</i> and targeted a bacterial indicator of the presence of the pathogen. These results could lead to a better risk assessment of the contamination of meat products.
format	article
author	Fanie Shedleur-Bourguignon William P. Thériault Jessie Longpré Alexandre Thibodeau Philippe Fravalo
author_facet	Fanie Shedleur-Bourguignon William P. Thériault Jessie Longpré Alexandre Thibodeau Philippe Fravalo
author_sort	Fanie Shedleur-Bourguignon
title	Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
title_short	Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
title_full	Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
title_fullStr	Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
title_full_unstemmed	Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada
title_sort	use of an ecosystem-based approach to shed light on the heterogeneity of the contamination pattern of <i>listeria monocytogenes</i> on conveyor belt surfaces in a swine slaughterhouse in the province of quebec, canada
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2987ebd4aa1244a68807c9c9d5ccd422
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Use of an Ecosystem-Based Approach to Shed Light on the Heterogeneity of the Contamination Pattern of <i>Listeria monocytogenes</i> on Conveyor Belt Surfaces in a Swine Slaughterhouse in the Province of Quebec, Canada

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