Ether lipid vesicle-based antigens impart protection against experimental listeriosis

Mairaj Ahmed Ansari,1 Swaleha Zubair,2 Saba Tufail,1 Ejaj Ahmad,1 Mohsin Raza Khan,1 Zainuddin Quadri,1 Mohammad Owais,11Interdisciplinary Biotechnology Unit, 2Women&#39;s College, Aligarh Muslim University, Aligarh, UP, IndiaBackground: Incidence of food-borne infections from Listeria monoc...

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Autores principales:	Ansari MA, Zubair S, Tufail S, Ahmad E, Khan MR, Quadri Z, Owais M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/29900fb5e81a4231a288745eda253f41
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spelling	oai:doaj.org-article:29900fb5e81a4231a288745eda253f412021-12-02T06:47:06ZEther lipid vesicle-based antigens impart protection against experimental listeriosis1176-91141178-2013https://doaj.org/article/29900fb5e81a4231a288745eda253f412012-06-01T00:00:00Zhttp://www.dovepress.com/ether-lipid-vesicle-based-antigens-impart-protection-against-experimen-a10051https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mairaj Ahmed Ansari,1 Swaleha Zubair,2 Saba Tufail,1 Ejaj Ahmad,1 Mohsin Raza Khan,1 Zainuddin Quadri,1 Mohammad Owais,11Interdisciplinary Biotechnology Unit, 2Women&#39;s College, Aligarh Muslim University, Aligarh, UP, IndiaBackground: Incidence of food-borne infections from Listeria monocytogenes, a parasite that has adapted intracellular residence to avoid antibody onslaught, has increased dramatically in the past few years. The apparent lack of an effective vaccine that is capable of evoking the desired cytotoxic T cell response to obliterate this intracellular pathogen has encouraged the investigation of alternate prophylactic strategies. It should also be noted that Archaebacteria (Archae) lipid-based adjuvants enhance the efficacy of subunit vaccines. In the present study, the adjuvant properties of archaeosomes (liposomes prepared from total polar lipids of archaebacteria, Halobacterium salinarum) combined with immunogenic culture supernatant antigens of L. monocytogenes have been exploited in designing a vaccine candidate against experimental listeriosis in murine model.Methods: Archaeosome-entrapped secretory protein antigens (SAgs) of L. monocytogenes were evaluated for their immunological responses and tendency to deplete bacterial burden in BALB/c mice challenged with sublethal listerial infection. Various immunological studies involving cytokine profiling, lymphocyte proliferation assay, detection of various surface markers (by flowcytometric analysis), and antibody isotypes (by enzyme-linked immunosorbent assay) were used for establishing the vaccine potential of archaeosome-entrapped secretory proteins.Results: Immunization schedule involving archaeosome-encapsulated SAgs resulted in upregulation of Th1 cytokine production along with boosted memory in BALB/c mice. It also showed protective effect by reducing listerial burden in various vital organs (liver and spleen) of the infected mice. However, the soluble form of the antigens (SAgs) and their physical mixture with sham (empty) archaeosomes, besides showing feeble Th1 response, were unable to protect the animals against virulent listerial infection.Conclusion: On the basis of the evidence provided by the current data, it is inferred that archaeosome-entrapped SAgs formulation not only enhances cytotoxic T cell response but also helps in the clearance of pathogens and thereby increases the survival of the immunized animals.Keywords: archaeosome, culture supernatant, antigen-presenting cells, Th1 cytokines, co-stimulatory markers, lymphocyte proliferation, protection studiesAnsari MAZubair STufail SAhmad EKhan MRQuadri ZOwais MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2433-2447 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ansari MA Zubair S Tufail S Ahmad E Khan MR Quadri Z Owais M Ether lipid vesicle-based antigens impart protection against experimental listeriosis
description	Mairaj Ahmed Ansari,1 Swaleha Zubair,2 Saba Tufail,1 Ejaj Ahmad,1 Mohsin Raza Khan,1 Zainuddin Quadri,1 Mohammad Owais,11Interdisciplinary Biotechnology Unit, 2Women&#39;s College, Aligarh Muslim University, Aligarh, UP, IndiaBackground: Incidence of food-borne infections from Listeria monocytogenes, a parasite that has adapted intracellular residence to avoid antibody onslaught, has increased dramatically in the past few years. The apparent lack of an effective vaccine that is capable of evoking the desired cytotoxic T cell response to obliterate this intracellular pathogen has encouraged the investigation of alternate prophylactic strategies. It should also be noted that Archaebacteria (Archae) lipid-based adjuvants enhance the efficacy of subunit vaccines. In the present study, the adjuvant properties of archaeosomes (liposomes prepared from total polar lipids of archaebacteria, Halobacterium salinarum) combined with immunogenic culture supernatant antigens of L. monocytogenes have been exploited in designing a vaccine candidate against experimental listeriosis in murine model.Methods: Archaeosome-entrapped secretory protein antigens (SAgs) of L. monocytogenes were evaluated for their immunological responses and tendency to deplete bacterial burden in BALB/c mice challenged with sublethal listerial infection. Various immunological studies involving cytokine profiling, lymphocyte proliferation assay, detection of various surface markers (by flowcytometric analysis), and antibody isotypes (by enzyme-linked immunosorbent assay) were used for establishing the vaccine potential of archaeosome-entrapped secretory proteins.Results: Immunization schedule involving archaeosome-encapsulated SAgs resulted in upregulation of Th1 cytokine production along with boosted memory in BALB/c mice. It also showed protective effect by reducing listerial burden in various vital organs (liver and spleen) of the infected mice. However, the soluble form of the antigens (SAgs) and their physical mixture with sham (empty) archaeosomes, besides showing feeble Th1 response, were unable to protect the animals against virulent listerial infection.Conclusion: On the basis of the evidence provided by the current data, it is inferred that archaeosome-entrapped SAgs formulation not only enhances cytotoxic T cell response but also helps in the clearance of pathogens and thereby increases the survival of the immunized animals.Keywords: archaeosome, culture supernatant, antigen-presenting cells, Th1 cytokines, co-stimulatory markers, lymphocyte proliferation, protection studies
format	article
author	Ansari MA Zubair S Tufail S Ahmad E Khan MR Quadri Z Owais M
author_facet	Ansari MA Zubair S Tufail S Ahmad E Khan MR Quadri Z Owais M
author_sort	Ansari MA
title	Ether lipid vesicle-based antigens impart protection against experimental listeriosis
title_short	Ether lipid vesicle-based antigens impart protection against experimental listeriosis
title_full	Ether lipid vesicle-based antigens impart protection against experimental listeriosis
title_fullStr	Ether lipid vesicle-based antigens impart protection against experimental listeriosis
title_full_unstemmed	Ether lipid vesicle-based antigens impart protection against experimental listeriosis
title_sort	ether lipid vesicle-based antigens impart protection against experimental listeriosis
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/29900fb5e81a4231a288745eda253f41
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Ether lipid vesicle-based antigens impart protection against experimental listeriosis

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