Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes

Mohammed Alfaifi,1,* Amit Kumar Verma,2,* Mohammad Yahya Alshahrani,1 Prakash C Joshi,2 Ali G Alkhathami,1 Irfan Ahmad,1 Abdulrahim Refdan Hakami,1 Mirza Masroor Ali Beg3,4 1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia; 2...

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Autores principales:	Alfaifi M, Verma AK, Alshahrani MY, Joshi PC, Alkhathami AG, Ahmad I, Hakami AR, Beg MMA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	diabetes lncrna microrna gene expression cell-free system. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/29909a54ed224a6eaad89d24a63e910b
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spelling	oai:doaj.org-article:29909a54ed224a6eaad89d24a63e910b2021-12-02T08:01:07ZAssessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes1178-7007https://doaj.org/article/29909a54ed224a6eaad89d24a63e910b2020-10-01T00:00:00Zhttps://www.dovepress.com/assessment-of-cell-free-long-non-coding-rna-h19-and-mirna-29a-mirna-29-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Mohammed Alfaifi,1,* Amit Kumar Verma,2,* Mohammad Yahya Alshahrani,1 Prakash C Joshi,2 Ali G Alkhathami,1 Irfan Ahmad,1 Abdulrahim Refdan Hakami,1 Mirza Masroor Ali Beg3,4 1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia; 2Department of Zoology and Environmental Sciences, GKV, Haridwar, India; 3Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India; 4Department of Biochemistry, Maulana Azad Medical College, New Delhi, India*These authors contributed equally to this workCorrespondence: Mirza Masroor Ali BegDepartment of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, IndiaTel +917827655616Email mirzamasroor1986@gmail.comBackground: Type 2 diabetes mellitus [T2DM] has been one of the common diseases and is characterized by increased blood glucose levels and suggested that cell-free non-coding RNAs and microRNAs (miRNAs) have been demonstrated to serve as important diagnostic/prognostic biomarkers in diabetes.Materials/Methods: The present study included clinically confirmed newly diagnosed 200 cases of T2DM and 200 healthy subjects, and all the parameters were taken care in diagnosis. Blood samples collected in plain vials were used for cell-free total RNA extraction and after that 100ng of total RNA was used to synthesize the cDNA for cell-free lncRNA H19, miRNA-29a, and miRNA-29b expression using quantitative real-time PCR method. Serum Biochemical parameters were analyzed after collection of the sample to observe the changes among T2DM cases and healthy controls.Results: It was observed that type 2 diabetic patients had decreased [0.59 fold] lncRNA H19 expression while increased miRNA-29a [5.62 fold] and miRNA-29b [5.58 fold] expression. Decreased expression of lncRNA H19 was observed to be associated with gender [p=0.004], hypertension [p< 0.0001], weight loss [p=0.02] and fatigue [p=0.02]. Increased miRNA29a expression was linked with hypertension [p< 0.0001], alcoholism [p=0.04], and smoking [p< 0.0001] as well as miRNA-29b expression was associated with hypertension [p=0.0001], weight loss [p=0.002], smoking [p=0.0002], alcoholism [p< 0.0001]. Low [≤ 1 fold] and high [> 1 fold] expression of lncRNA H19 expression was linked with miRNA-29a [p=0.005] and miRNA-29b [p< 0.0001] expression. lncRNA H19 expression showed negative correlation with miRNA-29a expression [r= − 27, p< 0.0001] and miRNA-29b [r= − 47, p< 0.0001].Conclusion: The present study concluded that lower lncRNA H19 expression, and increased miRNA-29b, a miRNA-29b expression associated with the severity of T2DM patients. Decreased lncRNA H19 expression, and increased miRNA-29b, miRNA-29b expression observed to be interrelated with clinicopathological findings of T2DM patients could involve in pathogenesis disease.Keywords: diabetes, lncRNA, microRNA, gene expression, cell-free systemAlfaifi MVerma AKAlshahrani MYJoshi PCAlkhathami AGAhmad IHakami ARBeg MMADove Medical Pressarticlediabeteslncrnamicrornagene expressioncell-free system.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 3727-3737 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes lncrna microrna gene expression cell-free system. Specialties of internal medicine RC581-951
spellingShingle	diabetes lncrna microrna gene expression cell-free system. Specialties of internal medicine RC581-951 Alfaifi M Verma AK Alshahrani MY Joshi PC Alkhathami AG Ahmad I Hakami AR Beg MMA Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
description	Mohammed Alfaifi,1,* Amit Kumar Verma,2,* Mohammad Yahya Alshahrani,1 Prakash C Joshi,2 Ali G Alkhathami,1 Irfan Ahmad,1 Abdulrahim Refdan Hakami,1 Mirza Masroor Ali Beg3,4 1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia; 2Department of Zoology and Environmental Sciences, GKV, Haridwar, India; 3Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India; 4Department of Biochemistry, Maulana Azad Medical College, New Delhi, India*These authors contributed equally to this workCorrespondence: Mirza Masroor Ali BegDepartment of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, IndiaTel +917827655616Email mirzamasroor1986@gmail.comBackground: Type 2 diabetes mellitus [T2DM] has been one of the common diseases and is characterized by increased blood glucose levels and suggested that cell-free non-coding RNAs and microRNAs (miRNAs) have been demonstrated to serve as important diagnostic/prognostic biomarkers in diabetes.Materials/Methods: The present study included clinically confirmed newly diagnosed 200 cases of T2DM and 200 healthy subjects, and all the parameters were taken care in diagnosis. Blood samples collected in plain vials were used for cell-free total RNA extraction and after that 100ng of total RNA was used to synthesize the cDNA for cell-free lncRNA H19, miRNA-29a, and miRNA-29b expression using quantitative real-time PCR method. Serum Biochemical parameters were analyzed after collection of the sample to observe the changes among T2DM cases and healthy controls.Results: It was observed that type 2 diabetic patients had decreased [0.59 fold] lncRNA H19 expression while increased miRNA-29a [5.62 fold] and miRNA-29b [5.58 fold] expression. Decreased expression of lncRNA H19 was observed to be associated with gender [p=0.004], hypertension [p< 0.0001], weight loss [p=0.02] and fatigue [p=0.02]. Increased miRNA29a expression was linked with hypertension [p< 0.0001], alcoholism [p=0.04], and smoking [p< 0.0001] as well as miRNA-29b expression was associated with hypertension [p=0.0001], weight loss [p=0.002], smoking [p=0.0002], alcoholism [p< 0.0001]. Low [≤ 1 fold] and high [> 1 fold] expression of lncRNA H19 expression was linked with miRNA-29a [p=0.005] and miRNA-29b [p< 0.0001] expression. lncRNA H19 expression showed negative correlation with miRNA-29a expression [r= − 27, p< 0.0001] and miRNA-29b [r= − 47, p< 0.0001].Conclusion: The present study concluded that lower lncRNA H19 expression, and increased miRNA-29b, a miRNA-29b expression associated with the severity of T2DM patients. Decreased lncRNA H19 expression, and increased miRNA-29b, miRNA-29b expression observed to be interrelated with clinicopathological findings of T2DM patients could involve in pathogenesis disease.Keywords: diabetes, lncRNA, microRNA, gene expression, cell-free system
format	article
author	Alfaifi M Verma AK Alshahrani MY Joshi PC Alkhathami AG Ahmad I Hakami AR Beg MMA
author_facet	Alfaifi M Verma AK Alshahrani MY Joshi PC Alkhathami AG Ahmad I Hakami AR Beg MMA
author_sort	Alfaifi M
title	Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
title_short	Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
title_full	Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
title_fullStr	Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
title_full_unstemmed	Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes
title_sort	assessment of cell-free long non-coding rna-h19 and mirna-29a, mirna-29b expression and severity of diabetes
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/29909a54ed224a6eaad89d24a63e910b
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Assessment of Cell-Free Long Non-Coding RNA-H19 and miRNA-29a, miRNA-29b Expression and Severity of Diabetes

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