Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle

Maria A K Schwartz1, John C Lieske2, Vivek Kumar2, Gerard Farell-Baril2, Virginia M Miller1,31Departments of Physiology and Biomedical Engineering, Internal Medicine; 2Division of Nephrology, and 3Surgery, Mayo Clinic College of Medicine, Rochester, MN, USAAbstract: Self-calcifying, self-replicating...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Maria A K Schwartz, John C Lieske, Vivek Kumar, Gerard Farell-Baril, Virginia M Miller
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/299671bc75bf4553aefcadac5a705b2c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:299671bc75bf4553aefcadac5a705b2c
record_format	dspace
spelling	oai:doaj.org-article:299671bc75bf4553aefcadac5a705b2c2021-12-02T02:31:37ZHuman-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle1176-91141178-2013https://doaj.org/article/299671bc75bf4553aefcadac5a705b2c2008-06-01T00:00:00Zhttp://www.dovepress.com/human-derived-nanoparticles-and-vascular-response-to-injury-in-rabbit--a1842https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Maria A K Schwartz1, John C Lieske2, Vivek Kumar2, Gerard Farell-Baril2, Virginia M Miller1,31Departments of Physiology and Biomedical Engineering, Internal Medicine; 2Division of Nephrology, and 3Surgery, Mayo Clinic College of Medicine, Rochester, MN, USAAbstract: Self-calcifying, self-replicating nanoparticles have been isolated from calcified human tissues. However, it is unclear if these nanoparticles participate in disease processes. Therefore, this study was designed to preliminarily test the hypothesis that human-derived nanoparticles are causal to arterial disease processes. One carotid artery of 3 kg male rabbits was denuded of endothelium; the contralateral artery remained unoperated as a control. Each rabbit was injected intravenously with either saline, calcified, or decalcified nanoparticles cultured from calcified human arteries or kidney stones. After 35 days, both injured and control arteries were removed for histological examination. Injured arteries from rabbits injected with saline showed minimal, eccentric intimal hyperplasia. Injured arteries from rabbits injected with calcified kidney stone- and arterial-derived nanoparticles occluded, sometimes with canalization. The calcified kidney stone-derived nanoparticles caused calcifications within the occlusion. Responses to injury in rabbits injected with decalcified kidney stone-derived nanoparticles were similar to those observed in saline-injected animals. However, decalcified arterial-derived nanoparticles produced intimal hyperplasia that varied from moderate to occlusion with canalization and calcifi cation. This study offers the first evidence that there may be a causal relationship between human-derived nanoparticles and response to injury including calcification in arteries with damaged endothelium.Keywords: arterial calcification, endothelial injury, intimal hyperplasia Maria A K SchwartzJohn C LieskeVivek KumarGerard Farell-BarilVirginia M MillerDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 2, Pp 243-248 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Maria A K Schwartz John C Lieske Vivek Kumar Gerard Farell-Baril Virginia M Miller Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle
description	Maria A K Schwartz1, John C Lieske2, Vivek Kumar2, Gerard Farell-Baril2, Virginia M Miller1,31Departments of Physiology and Biomedical Engineering, Internal Medicine; 2Division of Nephrology, and 3Surgery, Mayo Clinic College of Medicine, Rochester, MN, USAAbstract: Self-calcifying, self-replicating nanoparticles have been isolated from calcified human tissues. However, it is unclear if these nanoparticles participate in disease processes. Therefore, this study was designed to preliminarily test the hypothesis that human-derived nanoparticles are causal to arterial disease processes. One carotid artery of 3 kg male rabbits was denuded of endothelium; the contralateral artery remained unoperated as a control. Each rabbit was injected intravenously with either saline, calcified, or decalcified nanoparticles cultured from calcified human arteries or kidney stones. After 35 days, both injured and control arteries were removed for histological examination. Injured arteries from rabbits injected with saline showed minimal, eccentric intimal hyperplasia. Injured arteries from rabbits injected with calcified kidney stone- and arterial-derived nanoparticles occluded, sometimes with canalization. The calcified kidney stone-derived nanoparticles caused calcifications within the occlusion. Responses to injury in rabbits injected with decalcified kidney stone-derived nanoparticles were similar to those observed in saline-injected animals. However, decalcified arterial-derived nanoparticles produced intimal hyperplasia that varied from moderate to occlusion with canalization and calcifi cation. This study offers the first evidence that there may be a causal relationship between human-derived nanoparticles and response to injury including calcification in arteries with damaged endothelium.Keywords: arterial calcification, endothelial injury, intimal hyperplasia
format	article
author	Maria A K Schwartz John C Lieske Vivek Kumar Gerard Farell-Baril Virginia M Miller
author_facet	Maria A K Schwartz John C Lieske Vivek Kumar Gerard Farell-Baril Virginia M Miller
author_sort	Maria A K Schwartz
title	Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle
title_short	Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle
title_full	Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle
title_fullStr	Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle
title_full_unstemmed	Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle
title_sort	human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: proof of principle
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/299671bc75bf4553aefcadac5a705b2c
work_keys_str_mv	AT mariaakschwartz humanderivednanoparticlesandvascularresponsetoinjuryinrabbitcarotidarteriesproofofprinciple AT johnclieske humanderivednanoparticlesandvascularresponsetoinjuryinrabbitcarotidarteriesproofofprinciple AT vivekkumar humanderivednanoparticlesandvascularresponsetoinjuryinrabbitcarotidarteriesproofofprinciple AT gerardfarellbaril humanderivednanoparticlesandvascularresponsetoinjuryinrabbitcarotidarteriesproofofprinciple AT virginiammiller humanderivednanoparticlesandvascularresponsetoinjuryinrabbitcarotidarteriesproofofprinciple
_version_	1718402398594007040

Human-derived nanoparticles and vascular response to injury in rabbit carotid arteries: Proof of principle

Ejemplares similares