Susceptibility of Field-Collected Nyssorhynchus darlingi to Plasmodium spp. in Western Amazonian Brazil

Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynami...

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Autores principales:	Diego Peres Alonso, Marcus Vinicius Niz Alvarez, Paulo Eduardo Martins Ribolla, Jan E. Conn, Tatiane Marques Porangaba de Oliveira, Maria Anice Mureb Sallum
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Nyssorhynchus</i> <i>darlingi</i> GWAS NextRAD cytochrome P450 chitinase Genetics QH426-470
Acceso en línea:	https://doaj.org/article/299911c526474564a2a7a87185742ee1
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spelling	oai:doaj.org-article:299911c526474564a2a7a87185742ee12021-11-25T17:40:53ZSusceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil10.3390/genes121116932073-4425https://doaj.org/article/299911c526474564a2a7a87185742ee12021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1693https://doaj.org/toc/2073-4425Mosquito susceptibility to <i>Plasmodium</i> spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of <i>Nyssorhynchus darlingi</i>—the dominant malaria vector in Brazil—to <i>Plasmodium</i> spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to <i>Plasmodium</i> infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in <i>Ny.</i> <i>darlingi</i> infected with <i>Plasmodium</i> spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to <i>Plasmodium</i> infection: Cytochrome P450 (<i>cyp450</i>) and <i>chitinase.</i> This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to <i>Plasmodium</i> infection in <i>Ny. darlingi</i> in western Amazonian Brazil.Diego Peres AlonsoMarcus Vinicius Niz AlvarezPaulo Eduardo Martins RibollaJan E. ConnTatiane Marques Porangaba de OliveiraMaria Anice Mureb SallumMDPI AGarticle<i>Nyssorhynchus</i> <i>darlingi</i>GWASNextRADcytochrome P450chitinaseGeneticsQH426-470ENGenes, Vol 12, Iss 1693, p 1693 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Nyssorhynchus</i> <i>darlingi</i> GWAS NextRAD cytochrome P450 chitinase Genetics QH426-470
spellingShingle	<i>Nyssorhynchus</i> <i>darlingi</i> GWAS NextRAD cytochrome P450 chitinase Genetics QH426-470 Diego Peres Alonso Marcus Vinicius Niz Alvarez Paulo Eduardo Martins Ribolla Jan E. Conn Tatiane Marques Porangaba de Oliveira Maria Anice Mureb Sallum Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil
description	Mosquito susceptibility to <i>Plasmodium</i> spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of <i>Nyssorhynchus darlingi</i>—the dominant malaria vector in Brazil—to <i>Plasmodium</i> spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to <i>Plasmodium</i> infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in <i>Ny.</i> <i>darlingi</i> infected with <i>Plasmodium</i> spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to <i>Plasmodium</i> infection: Cytochrome P450 (<i>cyp450</i>) and <i>chitinase.</i> This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to <i>Plasmodium</i> infection in <i>Ny. darlingi</i> in western Amazonian Brazil.
format	article
author	Diego Peres Alonso Marcus Vinicius Niz Alvarez Paulo Eduardo Martins Ribolla Jan E. Conn Tatiane Marques Porangaba de Oliveira Maria Anice Mureb Sallum
author_facet	Diego Peres Alonso Marcus Vinicius Niz Alvarez Paulo Eduardo Martins Ribolla Jan E. Conn Tatiane Marques Porangaba de Oliveira Maria Anice Mureb Sallum
author_sort	Diego Peres Alonso
title	Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil
title_short	Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil
title_full	Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil
title_fullStr	Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil
title_full_unstemmed	Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil
title_sort	susceptibility of field-collected <i>nyssorhynchus darlingi</i> to <i>plasmodium</i> spp. in western amazonian brazil
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/299911c526474564a2a7a87185742ee1
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Susceptibility of Field-Collected <i>Nyssorhynchus darlingi</i> to <i>Plasmodium</i> spp. in Western Amazonian Brazil

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