Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors

Abstract Cyanide-based blood poisoning can seriously damage fire victims and cause death if not detected quickly. Previous conventional methods require laboratory equipment, which are expensive and increase the duration of the analysis. Here, a simple origami based microfluidic device was introduced...

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Autores principales:	Azarmidokht Sheini, Marzieh Dadkhah Aseman, Mohammad Mahdi Bordbar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29a119c3ee1246f5aeb8e3f69b3774aa
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spelling	oai:doaj.org-article:29a119c3ee1246f5aeb8e3f69b3774aa2021-12-02T13:30:34ZOrigami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors10.1038/s41598-021-83186-02045-2322https://doaj.org/article/29a119c3ee1246f5aeb8e3f69b3774aa2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83186-0https://doaj.org/toc/2045-2322Abstract Cyanide-based blood poisoning can seriously damage fire victims and cause death if not detected quickly. Previous conventional methods require laboratory equipment, which are expensive and increase the duration of the analysis. Here, a simple origami based microfluidic device was introduced for point of need detection of blood cyanide concentration in people involved in fire. The device is made of four layers of paper. Each layer was in the size of 1 × 1 cm folded on each other. In this work, the blood sample was acidified by trichloroacetic acid to separate cyanide from methaemoglobin in the form of HCN gas. The produced gas released into borate buffer to recover free cyanide ions which interacted with the Pt complex ([Pt(p-MeC6H4)2(phen)]) used as a receptor in this study. Optimized conditions were applied to have a suitable interaction causing the color of the receptor to change from yellow to colorless. The color changes were recorded by a smartphone, and the sensor response was calculated by the routine image analysis software. The assay was capable of determining cyanide ions at different concentrations in the range of 1.0 to 100.0 µmol L−1. The detection limit of these determination was equal to 0.4 µmol L−1. The assay responses were not affected by the interfering species. As a practical analysis, the proposed sensor was applied to determine cyanide ions in the blood sample of 20 studied fire survivors and 10 controls with high accuracy.Azarmidokht SheiniMarzieh Dadkhah AsemanMohammad Mahdi BordbarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Azarmidokht Sheini Marzieh Dadkhah Aseman Mohammad Mahdi Bordbar Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
description	Abstract Cyanide-based blood poisoning can seriously damage fire victims and cause death if not detected quickly. Previous conventional methods require laboratory equipment, which are expensive and increase the duration of the analysis. Here, a simple origami based microfluidic device was introduced for point of need detection of blood cyanide concentration in people involved in fire. The device is made of four layers of paper. Each layer was in the size of 1 × 1 cm folded on each other. In this work, the blood sample was acidified by trichloroacetic acid to separate cyanide from methaemoglobin in the form of HCN gas. The produced gas released into borate buffer to recover free cyanide ions which interacted with the Pt complex ([Pt(p-MeC6H4)2(phen)]) used as a receptor in this study. Optimized conditions were applied to have a suitable interaction causing the color of the receptor to change from yellow to colorless. The color changes were recorded by a smartphone, and the sensor response was calculated by the routine image analysis software. The assay was capable of determining cyanide ions at different concentrations in the range of 1.0 to 100.0 µmol L−1. The detection limit of these determination was equal to 0.4 µmol L−1. The assay responses were not affected by the interfering species. As a practical analysis, the proposed sensor was applied to determine cyanide ions in the blood sample of 20 studied fire survivors and 10 controls with high accuracy.
format	article
author	Azarmidokht Sheini Marzieh Dadkhah Aseman Mohammad Mahdi Bordbar
author_facet	Azarmidokht Sheini Marzieh Dadkhah Aseman Mohammad Mahdi Bordbar
author_sort	Azarmidokht Sheini
title	Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
title_short	Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
title_full	Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
title_fullStr	Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
title_full_unstemmed	Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
title_sort	origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/29a119c3ee1246f5aeb8e3f69b3774aa
work_keys_str_mv	AT azarmidokhtsheini origamipaperanalyticalassaybasedonmetalcomplexsensorforrapiddeterminationofbloodcyanideconcentrationinfiresurvivors AT marziehdadkhahaseman origamipaperanalyticalassaybasedonmetalcomplexsensorforrapiddeterminationofbloodcyanideconcentrationinfiresurvivors AT mohammadmahdibordbar origamipaperanalyticalassaybasedonmetalcomplexsensorforrapiddeterminationofbloodcyanideconcentrationinfiresurvivors
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Origami paper analytical assay based on metal complex sensor for rapid determination of blood cyanide concentration in fire survivors

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