Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans

Attachment of the oligosaccharide Man9GlcNAc2 is required for glycoprotein folding and secretion but synthesizing this compound for structural and functional studies has remained challenging. Here, the authors achieve efficient Man9GlcNAc2 synthesis by reconstituting its biosynthetic pathway in vitr...

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Autores principales:	Sheng-Tao Li, Tian-Tian Lu, Xin-Xin Xu, Yi Ding, Zijie Li, Toshihiko Kitajima, Neta Dean, Ning Wang, Xiao-Dong Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/29a5c7cd42d945a9960434571e434f91
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spelling	oai:doaj.org-article:29a5c7cd42d945a9960434571e434f912021-12-02T16:57:11ZReconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans10.1038/s41467-019-09752-32041-1723https://doaj.org/article/29a5c7cd42d945a9960434571e434f912019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09752-3https://doaj.org/toc/2041-1723Attachment of the oligosaccharide Man9GlcNAc2 is required for glycoprotein folding and secretion but synthesizing this compound for structural and functional studies has remained challenging. Here, the authors achieve efficient Man9GlcNAc2 synthesis by reconstituting its biosynthetic pathway in vitro.Sheng-Tao LiTian-Tian LuXin-Xin XuYi DingZijie LiToshihiko KitajimaNeta DeanNing WangXiao-Dong GaoNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sheng-Tao Li Tian-Tian Lu Xin-Xin Xu Yi Ding Zijie Li Toshihiko Kitajima Neta Dean Ning Wang Xiao-Dong Gao Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
description	Attachment of the oligosaccharide Man9GlcNAc2 is required for glycoprotein folding and secretion but synthesizing this compound for structural and functional studies has remained challenging. Here, the authors achieve efficient Man9GlcNAc2 synthesis by reconstituting its biosynthetic pathway in vitro.
format	article
author	Sheng-Tao Li Tian-Tian Lu Xin-Xin Xu Yi Ding Zijie Li Toshihiko Kitajima Neta Dean Ning Wang Xiao-Dong Gao
author_facet	Sheng-Tao Li Tian-Tian Lu Xin-Xin Xu Yi Ding Zijie Li Toshihiko Kitajima Neta Dean Ning Wang Xiao-Dong Gao
author_sort	Sheng-Tao Li
title	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
title_short	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
title_full	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
title_fullStr	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
title_full_unstemmed	Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans
title_sort	reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose n-glycans
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/29a5c7cd42d945a9960434571e434f91
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Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans

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