Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System

Microbial communities in incipient soil systems serve as the only biotic force shaping landscape evolution. However, the underlying ecological forces shaping microbial community structure and function are inadequately understood. We used amplicon sequencing to determine microbial taxonomic assembly...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aditi Sengupta, Till H. M. Volkmann, Robert E. Danczak, James C. Stegen, Katerina Dontsova, Nate Abramson, Aaron S. Bugaj, Michael J. Volk, Katarena A. Matos, Antonio A. Meira-Neto, Albert Barberán, Julia W. Neilson, Raina M. Maier, Jon Chorover, Peter A. Troch, Laura K. Meredith
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	metagenome incipient soil community assembly landscape evolution 16S amplicon sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/29a6749f3c4b402f88eea71209582aff
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29a6749f3c4b402f88eea71209582aff
record_format	dspace
spelling	oai:doaj.org-article:29a6749f3c4b402f88eea71209582aff2021-11-30T12:53:01ZContrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System1664-302X10.3389/fmicb.2021.754698https://doaj.org/article/29a6749f3c4b402f88eea71209582aff2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.754698/fullhttps://doaj.org/toc/1664-302XMicrobial communities in incipient soil systems serve as the only biotic force shaping landscape evolution. However, the underlying ecological forces shaping microbial community structure and function are inadequately understood. We used amplicon sequencing to determine microbial taxonomic assembly and metagenome sequencing to evaluate microbial functional assembly in incipient basaltic soil subjected to precipitation. Community composition was stratified with soil depth in the pre-precipitation samples, with surficial communities maintaining their distinct structure and diversity after precipitation, while the deeper soil samples appeared to become more uniform. The structural community assembly remained deterministic in pre- and post-precipitation periods, with homogenous selection being dominant. Metagenome analysis revealed that carbon and nitrogen functional potential was assembled stochastically. Sub-populations putatively involved in the nitrogen cycle and carbon fixation experienced counteracting assembly pressures at the deepest depths, suggesting the communities may functionally assemble to respond to short-term environmental fluctuations and impact the landscape-scale response to perturbations. We propose that contrasting assembly forces impact microbial structure and potential function in an incipient landscape; in situ landscape characteristics (here homogenous parent material) drive community structure assembly, while short-term environmental fluctuations (here precipitation) shape environmental variations that are random in the soil depth profile and drive stochastic sub-population functional dynamics.Aditi SenguptaAditi SenguptaTill H. M. VolkmannRobert E. DanczakJames C. StegenKaterina DontsovaNate AbramsonAaron S. BugajMichael J. VolkKatarena A. MatosAntonio A. Meira-NetoAlbert BarberánJulia W. NeilsonRaina M. MaierJon ChoroverPeter A. TrochLaura K. MeredithFrontiers Media S.A.articlemetagenomeincipient soilcommunity assemblylandscape evolution16S amplicon sequencingMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	metagenome incipient soil community assembly landscape evolution 16S amplicon sequencing Microbiology QR1-502
spellingShingle	metagenome incipient soil community assembly landscape evolution 16S amplicon sequencing Microbiology QR1-502 Aditi Sengupta Aditi Sengupta Till H. M. Volkmann Robert E. Danczak James C. Stegen Katerina Dontsova Nate Abramson Aaron S. Bugaj Michael J. Volk Katarena A. Matos Antonio A. Meira-Neto Albert Barberán Julia W. Neilson Raina M. Maier Jon Chorover Peter A. Troch Laura K. Meredith Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System
description	Microbial communities in incipient soil systems serve as the only biotic force shaping landscape evolution. However, the underlying ecological forces shaping microbial community structure and function are inadequately understood. We used amplicon sequencing to determine microbial taxonomic assembly and metagenome sequencing to evaluate microbial functional assembly in incipient basaltic soil subjected to precipitation. Community composition was stratified with soil depth in the pre-precipitation samples, with surficial communities maintaining their distinct structure and diversity after precipitation, while the deeper soil samples appeared to become more uniform. The structural community assembly remained deterministic in pre- and post-precipitation periods, with homogenous selection being dominant. Metagenome analysis revealed that carbon and nitrogen functional potential was assembled stochastically. Sub-populations putatively involved in the nitrogen cycle and carbon fixation experienced counteracting assembly pressures at the deepest depths, suggesting the communities may functionally assemble to respond to short-term environmental fluctuations and impact the landscape-scale response to perturbations. We propose that contrasting assembly forces impact microbial structure and potential function in an incipient landscape; in situ landscape characteristics (here homogenous parent material) drive community structure assembly, while short-term environmental fluctuations (here precipitation) shape environmental variations that are random in the soil depth profile and drive stochastic sub-population functional dynamics.
format	article
author	Aditi Sengupta Aditi Sengupta Till H. M. Volkmann Robert E. Danczak James C. Stegen Katerina Dontsova Nate Abramson Aaron S. Bugaj Michael J. Volk Katarena A. Matos Antonio A. Meira-Neto Albert Barberán Julia W. Neilson Raina M. Maier Jon Chorover Peter A. Troch Laura K. Meredith
author_facet	Aditi Sengupta Aditi Sengupta Till H. M. Volkmann Robert E. Danczak James C. Stegen Katerina Dontsova Nate Abramson Aaron S. Bugaj Michael J. Volk Katarena A. Matos Antonio A. Meira-Neto Albert Barberán Julia W. Neilson Raina M. Maier Jon Chorover Peter A. Troch Laura K. Meredith
author_sort	Aditi Sengupta
title	Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System
title_short	Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System
title_full	Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System
title_fullStr	Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System
title_full_unstemmed	Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System
title_sort	contrasting community assembly forces drive microbial structural and potential functional responses to precipitation in an incipient soil system
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/29a6749f3c4b402f88eea71209582aff
work_keys_str_mv	AT aditisengupta contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT aditisengupta contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT tillhmvolkmann contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT robertedanczak contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT jamescstegen contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT katerinadontsova contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT nateabramson contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT aaronsbugaj contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT michaeljvolk contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT katarenaamatos contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT antonioameiraneto contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT albertbarberan contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT juliawneilson contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT rainammaier contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT jonchorover contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT peteratroch contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem AT laurakmeredith contrastingcommunityassemblyforcesdrivemicrobialstructuralandpotentialfunctionalresponsestoprecipitationinanincipientsoilsystem
_version_	1718406552789975040

Contrasting Community Assembly Forces Drive Microbial Structural and Potential Functional Responses to Precipitation in an Incipient Soil System

Ejemplares similares