Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete

Abstract Concrete cracks must be repaired promptly in order to prevent structural damage and to prolong the structural life of the building (or other such construction). Biological self-healing concrete is a recent alternative technology involving the biochemical reaction of microbial induced calciu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wiboonluk Pungrasmi, Jirapa Intarasoontron, Pitcha Jongvivatsakul, Suched Likitlersuang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29b074ce21dd4aa082e817a720473304
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29b074ce21dd4aa082e817a720473304
record_format	dspace
spelling	oai:doaj.org-article:29b074ce21dd4aa082e817a7204733042021-12-02T15:08:08ZEvaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete10.1038/s41598-019-49002-62045-2322https://doaj.org/article/29b074ce21dd4aa082e817a7204733042019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49002-6https://doaj.org/toc/2045-2322Abstract Concrete cracks must be repaired promptly in order to prevent structural damage and to prolong the structural life of the building (or other such construction). Biological self-healing concrete is a recent alternative technology involving the biochemical reaction of microbial induced calcium carbonate precipitation (MICP). This study determined the most appropriate technique to encapsulate spores of Bacillus sphaericus LMG 22257 with sodium alginate so as to protect the bacterial spores during the concrete mixing and hardening period. Three techniques (extrusion, spray drying and freeze drying) to encapsulate the bacterial spores with sodium alginate were evaluated. The freeze-drying process provided the highest bacterial spore survival rate (100%), while the extruded and spray-dried processes had a lower spore survival rate of 93.8% and 79.9%, respectively. To investigate the viability of microencapsulated spores after being mixed with mortar, the decomposed urea analysis was conducted. The results revealed that the freeze-dried spores also showed the highest level of urea decomposition (metabolic activity assay used as a surrogate marker of spore germination and vegetative cell viability). Thus, the self-healing performance of concrete mixed with freeze-dried spores was evaluated. The results showed that the crack healing ratio observed from the mortar specimens with freeze-dried microencapsulated spores were significantly higher than those without bacteria. This study revealed that freeze drying has a high potential as a microencapsulation technique for application to self-healing concrete technology.Wiboonluk PungrasmiJirapa IntarasoontronPitcha JongvivatsakulSuched LikitlersuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wiboonluk Pungrasmi Jirapa Intarasoontron Pitcha Jongvivatsakul Suched Likitlersuang Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete
description	Abstract Concrete cracks must be repaired promptly in order to prevent structural damage and to prolong the structural life of the building (or other such construction). Biological self-healing concrete is a recent alternative technology involving the biochemical reaction of microbial induced calcium carbonate precipitation (MICP). This study determined the most appropriate technique to encapsulate spores of Bacillus sphaericus LMG 22257 with sodium alginate so as to protect the bacterial spores during the concrete mixing and hardening period. Three techniques (extrusion, spray drying and freeze drying) to encapsulate the bacterial spores with sodium alginate were evaluated. The freeze-drying process provided the highest bacterial spore survival rate (100%), while the extruded and spray-dried processes had a lower spore survival rate of 93.8% and 79.9%, respectively. To investigate the viability of microencapsulated spores after being mixed with mortar, the decomposed urea analysis was conducted. The results revealed that the freeze-dried spores also showed the highest level of urea decomposition (metabolic activity assay used as a surrogate marker of spore germination and vegetative cell viability). Thus, the self-healing performance of concrete mixed with freeze-dried spores was evaluated. The results showed that the crack healing ratio observed from the mortar specimens with freeze-dried microencapsulated spores were significantly higher than those without bacteria. This study revealed that freeze drying has a high potential as a microencapsulation technique for application to self-healing concrete technology.
format	article
author	Wiboonluk Pungrasmi Jirapa Intarasoontron Pitcha Jongvivatsakul Suched Likitlersuang
author_facet	Wiboonluk Pungrasmi Jirapa Intarasoontron Pitcha Jongvivatsakul Suched Likitlersuang
author_sort	Wiboonluk Pungrasmi
title	Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete
title_short	Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete
title_full	Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete
title_fullStr	Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete
title_full_unstemmed	Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete
title_sort	evaluation of microencapsulation techniques for micp bacterial spores applied in self-healing concrete
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/29b074ce21dd4aa082e817a720473304
work_keys_str_mv	AT wiboonlukpungrasmi evaluationofmicroencapsulationtechniquesformicpbacterialsporesappliedinselfhealingconcrete AT jirapaintarasoontron evaluationofmicroencapsulationtechniquesformicpbacterialsporesappliedinselfhealingconcrete AT pitchajongvivatsakul evaluationofmicroencapsulationtechniquesformicpbacterialsporesappliedinselfhealingconcrete AT suchedlikitlersuang evaluationofmicroencapsulationtechniquesformicpbacterialsporesappliedinselfhealingconcrete
_version_	1718388240491216896

Evaluation of Microencapsulation Techniques for MICP Bacterial Spores Applied in Self-Healing Concrete

Ejemplares similares