Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound agains...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Damariz Marín-Palma, Jorge H. Tabares-Guevara, María I. Zapata-Cardona, Lizdany Flórez-Álvarez, Lina M. Yepes, Maria T. Rugeles, Wildeman Zapata-Builes, Juan C. Hernandez, Natalia A. Taborda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	curcumin antiviral COVID-19 SARS-CoV-2 immune response inflammation Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/29b0d58c76cf49e0a6467eb1e1065e3c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29b0d58c76cf49e0a6467eb1e1065e3c
record_format	dspace
spelling	oai:doaj.org-article:29b0d58c76cf49e0a6467eb1e1065e3c2021-11-25T18:28:14ZCurcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms10.3390/molecules262269001420-3049https://doaj.org/article/29b0d58c76cf49e0a6467eb1e1065e3c2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6900https://doaj.org/toc/1420-3049Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound against SARS-CoV-2 was evaluated using four treatment strategies (i. pre–post infection treatment, ii. co-treatment, iii. pre-infection, and iv. post-infection). The D614G strain and Delta variant of SARS-CoV-2 were used, and the viral titer was quantified by plaque assay. The anti-inflammatory effect was evaluated in peripheral blood mononuclear cells (PBMCs) using qPCR and ELISA. By pre–post infection treatment, Curcumin (10 µg/mL) exhibited antiviral effect of 99% and 99.8% against DG614 strain and Delta variant, respectively. Curcumin also inhibited D614G strain by pre-infection and post-infection treatment. In addition, curcumin showed a virucidal effect against D614G strain and Delta variant. Finally, the pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) released by PBMCs triggered by SARS-CoV-2 were decreased after treatment with curcumin. Our results suggest that curcumin affects the SARS-CoV-2 replicative cycle and exhibits virucidal effect with a variant/strain independent antiviral effect and immune-modulatory properties. This is the first study that showed a combined (antiviral/anti-inflammatory) effect of curcumin during SARS-CoV-2 infection. However, additional studies are required to define its use as a treatment for the COVID-19.Damariz Marín-PalmaJorge H. Tabares-GuevaraMaría I. Zapata-CardonaLizdany Flórez-ÁlvarezLina M. YepesMaria T. RugelesWildeman Zapata-BuilesJuan C. HernandezNatalia A. TabordaMDPI AGarticlecurcuminantiviralCOVID-19SARS-CoV-2immune responseinflammationOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6900, p 6900 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	curcumin antiviral COVID-19 SARS-CoV-2 immune response inflammation Organic chemistry QD241-441
spellingShingle	curcumin antiviral COVID-19 SARS-CoV-2 immune response inflammation Organic chemistry QD241-441 Damariz Marín-Palma Jorge H. Tabares-Guevara María I. Zapata-Cardona Lizdany Flórez-Álvarez Lina M. Yepes Maria T. Rugeles Wildeman Zapata-Builes Juan C. Hernandez Natalia A. Taborda Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms
description	Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound against SARS-CoV-2 was evaluated using four treatment strategies (i. pre–post infection treatment, ii. co-treatment, iii. pre-infection, and iv. post-infection). The D614G strain and Delta variant of SARS-CoV-2 were used, and the viral titer was quantified by plaque assay. The anti-inflammatory effect was evaluated in peripheral blood mononuclear cells (PBMCs) using qPCR and ELISA. By pre–post infection treatment, Curcumin (10 µg/mL) exhibited antiviral effect of 99% and 99.8% against DG614 strain and Delta variant, respectively. Curcumin also inhibited D614G strain by pre-infection and post-infection treatment. In addition, curcumin showed a virucidal effect against D614G strain and Delta variant. Finally, the pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) released by PBMCs triggered by SARS-CoV-2 were decreased after treatment with curcumin. Our results suggest that curcumin affects the SARS-CoV-2 replicative cycle and exhibits virucidal effect with a variant/strain independent antiviral effect and immune-modulatory properties. This is the first study that showed a combined (antiviral/anti-inflammatory) effect of curcumin during SARS-CoV-2 infection. However, additional studies are required to define its use as a treatment for the COVID-19.
format	article
author	Damariz Marín-Palma Jorge H. Tabares-Guevara María I. Zapata-Cardona Lizdany Flórez-Álvarez Lina M. Yepes Maria T. Rugeles Wildeman Zapata-Builes Juan C. Hernandez Natalia A. Taborda
author_facet	Damariz Marín-Palma Jorge H. Tabares-Guevara María I. Zapata-Cardona Lizdany Flórez-Álvarez Lina M. Yepes Maria T. Rugeles Wildeman Zapata-Builes Juan C. Hernandez Natalia A. Taborda
author_sort	Damariz Marín-Palma
title	Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms
title_short	Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms
title_full	Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms
title_fullStr	Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms
title_full_unstemmed	Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms
title_sort	curcumin inhibits in vitro sars-cov-2 infection in vero e6 cells through multiple antiviral mechanisms
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/29b0d58c76cf49e0a6467eb1e1065e3c
work_keys_str_mv	AT damarizmarinpalma curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT jorgehtabaresguevara curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT mariaizapatacardona curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT lizdanyflorezalvarez curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT linamyepes curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT mariatrugeles curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT wildemanzapatabuiles curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT juanchernandez curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms AT nataliaataborda curcumininhibitsinvitrosarscov2infectioninveroe6cellsthroughmultipleantiviralmechanisms
_version_	1718411122432802816

Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

Ejemplares similares