Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease characterized by excessive fat accumulation in the liver. The aim of this study is to elucidate the multi-target mechanism of polyphenols in blueberry leaves (PBL) on NAFLD by network pharmacology and to validate its...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cai-Ren Wang, Hong-Wei Chen, Yan Li, Ming-Yue Zhou, Vincent Kam-Wai Wong, Zhi-Hong Jiang, Wei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polyphenols in blueberry leaves network pharmacology NAFLD apoptosis Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/29b84b64a0954cdb8ff9fd394c50d8e8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29b84b64a0954cdb8ff9fd394c50d8e8
record_format	dspace
spelling	oai:doaj.org-article:29b84b64a0954cdb8ff9fd394c50d8e82021-11-25T18:36:28ZNetwork Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols10.3390/nu131140602072-6643https://doaj.org/article/29b84b64a0954cdb8ff9fd394c50d8e82021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/4060https://doaj.org/toc/2072-6643Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease characterized by excessive fat accumulation in the liver. The aim of this study is to elucidate the multi-target mechanism of polyphenols in blueberry leaves (PBL) on NAFLD by network pharmacology and to validate its results via biological experiments. Twenty constituents in PBL were preliminarily determined by liquid chromatography-tandem mass spectrometry. Subsequently, 141 predicted drug targets and 1226 targets associated with NAFLD were retrieved from public databases, respectively. The herb-compound-target network and the target protein–protein interaction network (PPI) were established through Cytoscape software, and four compounds and 53 corresponding targets were identified. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed to explore the biological processes of the predicted genes. The results of cell experiments demonstrated that PBL could significantly improve the viability of the NAFLD cell model, and the protein expressions of caspase-3 and Bcl-2 were consistent with the expected mechanism of action of PBL. Those results systematically revealed that the multi-target mechanism of PBL against NAFLD was related to the apoptosis pathway, which could bring deeper reflections into the hepatoprotective effect of PBL.Cai-Ren WangHong-Wei ChenYan LiMing-Yue ZhouVincent Kam-Wai WongZhi-Hong JiangWei ZhangMDPI AGarticlepolyphenols in blueberry leavesnetwork pharmacologyNAFLDapoptosisNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 4060, p 4060 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyphenols in blueberry leaves network pharmacology NAFLD apoptosis Nutrition. Foods and food supply TX341-641
spellingShingle	polyphenols in blueberry leaves network pharmacology NAFLD apoptosis Nutrition. Foods and food supply TX341-641 Cai-Ren Wang Hong-Wei Chen Yan Li Ming-Yue Zhou Vincent Kam-Wai Wong Zhi-Hong Jiang Wei Zhang Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
description	Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease characterized by excessive fat accumulation in the liver. The aim of this study is to elucidate the multi-target mechanism of polyphenols in blueberry leaves (PBL) on NAFLD by network pharmacology and to validate its results via biological experiments. Twenty constituents in PBL were preliminarily determined by liquid chromatography-tandem mass spectrometry. Subsequently, 141 predicted drug targets and 1226 targets associated with NAFLD were retrieved from public databases, respectively. The herb-compound-target network and the target protein–protein interaction network (PPI) were established through Cytoscape software, and four compounds and 53 corresponding targets were identified. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were performed to explore the biological processes of the predicted genes. The results of cell experiments demonstrated that PBL could significantly improve the viability of the NAFLD cell model, and the protein expressions of caspase-3 and Bcl-2 were consistent with the expected mechanism of action of PBL. Those results systematically revealed that the multi-target mechanism of PBL against NAFLD was related to the apoptosis pathway, which could bring deeper reflections into the hepatoprotective effect of PBL.
format	article
author	Cai-Ren Wang Hong-Wei Chen Yan Li Ming-Yue Zhou Vincent Kam-Wai Wong Zhi-Hong Jiang Wei Zhang
author_facet	Cai-Ren Wang Hong-Wei Chen Yan Li Ming-Yue Zhou Vincent Kam-Wai Wong Zhi-Hong Jiang Wei Zhang
author_sort	Cai-Ren Wang
title	Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
title_short	Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
title_full	Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
title_fullStr	Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
title_full_unstemmed	Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols
title_sort	network pharmacology exploration reveals anti-apoptosis as a common therapeutic mechanism for non-alcoholic fatty liver disease treated with blueberry leaf polyphenols
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/29b84b64a0954cdb8ff9fd394c50d8e8
work_keys_str_mv	AT cairenwang networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols AT hongweichen networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols AT yanli networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols AT mingyuezhou networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols AT vincentkamwaiwong networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols AT zhihongjiang networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols AT weizhang networkpharmacologyexplorationrevealsantiapoptosisasacommontherapeuticmechanismfornonalcoholicfattyliverdiseasetreatedwithblueberryleafpolyphenols
_version_	1718410882070872064

Network Pharmacology Exploration Reveals Anti-Apoptosis as a Common Therapeutic Mechanism for Non-Alcoholic Fatty Liver Disease Treated with Blueberry Leaf Polyphenols

Ejemplares similares