Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress

Abstract The role of DNA methylation and its interaction with gene expression and transcriptome plasticity is poorly understood, and current insight comes mainly from studies in very few model plant species. Here, we study gene body DNA methylation (gbM) and gene expression patterns in ecotypes from...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Laura Entrambasaguas, Miriam Ruocco, Koen J. F. Verhoeven, Gabriele Procaccini, Lazaro Marín-Guirao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29baca68c1f84757a9fcad0f2dc18fa8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:29baca68c1f84757a9fcad0f2dc18fa8
record_format	dspace
spelling	oai:doaj.org-article:29baca68c1f84757a9fcad0f2dc18fa82021-12-02T18:30:39ZGene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress10.1038/s41598-021-93606-w2045-2322https://doaj.org/article/29baca68c1f84757a9fcad0f2dc18fa82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93606-whttps://doaj.org/toc/2045-2322Abstract The role of DNA methylation and its interaction with gene expression and transcriptome plasticity is poorly understood, and current insight comes mainly from studies in very few model plant species. Here, we study gene body DNA methylation (gbM) and gene expression patterns in ecotypes from contrasting thermal environments of two marine plants with contrasting life history strategies in order to explore the potential role epigenetic mechanisms could play in gene plasticity and responsiveness to heat stress. In silico transcriptome analysis of CpGO/E ratios suggested that the bulk of Posidonia oceanica and Cymodocea nodosa genes possess high levels of intragenic methylation. We also observed a correlation between gbM and gene expression flexibility: genes with low DNA methylation tend to show flexible gene expression and plasticity under changing conditions. Furthermore, the empirical determination of global DNA methylation (5-mC) showed patterns of intra and inter-specific divergence that suggests a link between methylation level and the plants’ latitude of origin and life history. Although we cannot discern whether gbM regulates gene expression or vice versa, or if other molecular mechanisms play a role in facilitating transcriptome responsiveness, our findings point to the existence of a relationship between gene responsiveness and gbM patterns in marine plants.Laura EntrambasaguasMiriam RuoccoKoen J. F. VerhoevenGabriele ProcacciniLazaro Marín-GuiraoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Entrambasaguas Miriam Ruocco Koen J. F. Verhoeven Gabriele Procaccini Lazaro Marín-Guirao Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
description	Abstract The role of DNA methylation and its interaction with gene expression and transcriptome plasticity is poorly understood, and current insight comes mainly from studies in very few model plant species. Here, we study gene body DNA methylation (gbM) and gene expression patterns in ecotypes from contrasting thermal environments of two marine plants with contrasting life history strategies in order to explore the potential role epigenetic mechanisms could play in gene plasticity and responsiveness to heat stress. In silico transcriptome analysis of CpGO/E ratios suggested that the bulk of Posidonia oceanica and Cymodocea nodosa genes possess high levels of intragenic methylation. We also observed a correlation between gbM and gene expression flexibility: genes with low DNA methylation tend to show flexible gene expression and plasticity under changing conditions. Furthermore, the empirical determination of global DNA methylation (5-mC) showed patterns of intra and inter-specific divergence that suggests a link between methylation level and the plants’ latitude of origin and life history. Although we cannot discern whether gbM regulates gene expression or vice versa, or if other molecular mechanisms play a role in facilitating transcriptome responsiveness, our findings point to the existence of a relationship between gene responsiveness and gbM patterns in marine plants.
format	article
author	Laura Entrambasaguas Miriam Ruocco Koen J. F. Verhoeven Gabriele Procaccini Lazaro Marín-Guirao
author_facet	Laura Entrambasaguas Miriam Ruocco Koen J. F. Verhoeven Gabriele Procaccini Lazaro Marín-Guirao
author_sort	Laura Entrambasaguas
title	Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
title_short	Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
title_full	Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
title_fullStr	Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
title_full_unstemmed	Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
title_sort	gene body dna methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/29baca68c1f84757a9fcad0f2dc18fa8
work_keys_str_mv	AT lauraentrambasaguas genebodydnamethylationinseagrassesinterandintraspecificdifferencesandinteractionwithtranscriptomeplasticityunderheatstress AT miriamruocco genebodydnamethylationinseagrassesinterandintraspecificdifferencesandinteractionwithtranscriptomeplasticityunderheatstress AT koenjfverhoeven genebodydnamethylationinseagrassesinterandintraspecificdifferencesandinteractionwithtranscriptomeplasticityunderheatstress AT gabrieleprocaccini genebodydnamethylationinseagrassesinterandintraspecificdifferencesandinteractionwithtranscriptomeplasticityunderheatstress AT lazaromaringuirao genebodydnamethylationinseagrassesinterandintraspecificdifferencesandinteractionwithtranscriptomeplasticityunderheatstress
_version_	1718377966033960960

Gene body DNA methylation in seagrasses: inter- and intraspecific differences and interaction with transcriptome plasticity under heat stress

Ejemplares similares