Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites

Exopolysaccharide (EPS400) from highest cholesterol removing (85.81±0.76%) Limosilactobacillus fermentum NCDC400 (LFNCDC400) was isolated, purified by anion-exhange chromatography. FTIR and NMR analysis revealed that EPS400 comprised of functional hydroxyl (O-H) and carboxyl (C=O) groups and glycosi...

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Autores principales:	Krushna Gawande, Mrugnayani Kolhekar, Manorama Kumari, Suman Kapila, Prateek Sharma, Syed Azmal Ali, Pradip V. Behare
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Exopolysaccharide EPS400 Limosilactobacillus fermentum NCDC400 Functional properties Gelling ability Cholesterol lowering Nutrition. Foods and food supply TX341-641 Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/29cbdaea76534ae6814bc38d5a94ca10
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spelling	oai:doaj.org-article:29cbdaea76534ae6814bc38d5a94ca102021-11-28T04:39:45ZLactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites2667-025910.1016/j.fhfh.2021.100042https://doaj.org/article/29cbdaea76534ae6814bc38d5a94ca102021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667025921000340https://doaj.org/toc/2667-0259Exopolysaccharide (EPS400) from highest cholesterol removing (85.81±0.76%) Limosilactobacillus fermentum NCDC400 (LFNCDC400) was isolated, purified by anion-exhange chromatography. FTIR and NMR analysis revealed that EPS400 comprised of functional hydroxyl (O-H) and carboxyl (C=O) groups and glycosidic bonds. G′ of 2% (w/v) EPS400 solution was higher than the G″, indicating its viscofying behavior relating to its heteropolysaccharides nature and greater MW (5.7 × 105 Da) as demonstrated earlier. The heteropolysaccharides molecule appeared with coarse surface and compact structure in SEM analysis. At a concentration of 2%, EPS400 removed 90.32±0.28% cholesterol from the media. EPS400 showed greater reduction in cholesterol bioaccessibility than the LFNCDC400, under gastrointestinal conditions. In animal studies, a known cholesterol reducing β-glucan and EPS400 significantly (P<0.05) lowered and maintained cholesterol profile in hypercholesterolemic rats than LFNCDC400. These findings suggest that EPS400 could be used to remove diatary cholesterol and serve as novel hypocholesterolemic agent for food and pharmaceutical applications.Krushna GawandeMrugnayani KolhekarManorama KumariSuman KapilaPrateek SharmaSyed Azmal AliPradip V. BehareElsevierarticleExopolysaccharideEPS400Limosilactobacillus fermentum NCDC400Functional propertiesGelling abilityCholesterol loweringNutrition. Foods and food supplyTX341-641Nutritional diseases. Deficiency diseasesRC620-627ENFood Hydrocolloids for Health, Vol 1, Iss , Pp 100042- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Exopolysaccharide EPS400 Limosilactobacillus fermentum NCDC400 Functional properties Gelling ability Cholesterol lowering Nutrition. Foods and food supply TX341-641 Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	Exopolysaccharide EPS400 Limosilactobacillus fermentum NCDC400 Functional properties Gelling ability Cholesterol lowering Nutrition. Foods and food supply TX341-641 Nutritional diseases. Deficiency diseases RC620-627 Krushna Gawande Mrugnayani Kolhekar Manorama Kumari Suman Kapila Prateek Sharma Syed Azmal Ali Pradip V. Behare Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
description	Exopolysaccharide (EPS400) from highest cholesterol removing (85.81±0.76%) Limosilactobacillus fermentum NCDC400 (LFNCDC400) was isolated, purified by anion-exhange chromatography. FTIR and NMR analysis revealed that EPS400 comprised of functional hydroxyl (O-H) and carboxyl (C=O) groups and glycosidic bonds. G′ of 2% (w/v) EPS400 solution was higher than the G″, indicating its viscofying behavior relating to its heteropolysaccharides nature and greater MW (5.7 × 105 Da) as demonstrated earlier. The heteropolysaccharides molecule appeared with coarse surface and compact structure in SEM analysis. At a concentration of 2%, EPS400 removed 90.32±0.28% cholesterol from the media. EPS400 showed greater reduction in cholesterol bioaccessibility than the LFNCDC400, under gastrointestinal conditions. In animal studies, a known cholesterol reducing β-glucan and EPS400 significantly (P<0.05) lowered and maintained cholesterol profile in hypercholesterolemic rats than LFNCDC400. These findings suggest that EPS400 could be used to remove diatary cholesterol and serve as novel hypocholesterolemic agent for food and pharmaceutical applications.
format	article
author	Krushna Gawande Mrugnayani Kolhekar Manorama Kumari Suman Kapila Prateek Sharma Syed Azmal Ali Pradip V. Behare
author_facet	Krushna Gawande Mrugnayani Kolhekar Manorama Kumari Suman Kapila Prateek Sharma Syed Azmal Ali Pradip V. Behare
author_sort	Krushna Gawande
title	Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
title_short	Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
title_full	Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
title_fullStr	Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
title_full_unstemmed	Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
title_sort	lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/29cbdaea76534ae6814bc38d5a94ca10
work_keys_str_mv	AT krushnagawande lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites AT mrugnayanikolhekar lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites AT manoramakumari lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites AT sumankapila lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites AT prateeksharma lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites AT syedazmalali lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites AT pradipvbehare lacticacidbacteriabasedpurifiedexopolysaccharideshowedviscofyingandhypercholesterolemiccapabilites
_version_	1718408248260820992

Lactic acid bacteria based purified exopolysaccharide showed viscofying and hypercholesterolemic capabilites

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