Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes

Kai Li,1,* Yongxing Zhang,2,* Mengting Chen,1 Yangyang Hu,1 Weiliang Jiang,1 Li Zhou,1 Sisi Li,1 Min Xu,1 Qinghua Zhao,2 Rong Wan1 1Department of Gastroenterology, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of...

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Autores principales:	Li K, Zhang Y, Chen M, Hu Y, Jiang W, Zhou L, Li S, Xu M, Zhao Q, Wan R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	HNTs DOX gastric cancer drug carrier Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/29d0cbe9b31c4a709317f37b29833724
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spelling	oai:doaj.org-article:29d0cbe9b31c4a709317f37b298337242021-12-02T00:12:17ZEnhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes1178-2013https://doaj.org/article/29d0cbe9b31c4a709317f37b298337242017-12-01T00:00:00Zhttps://www.dovepress.com/enhanced-antitumor-efficacy-of-doxorubicin-encapsulated-halloysite-nan-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kai Li,1,* Yongxing Zhang,2,* Mengting Chen,1 Yangyang Hu,1 Weiliang Jiang,1 Li Zhou,1 Sisi Li,1 Min Xu,1 Qinghua Zhao,2 Rong Wan1 1Department of Gastroenterology, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Department of Orthopaedics, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: To improve the antitumor efficacy of doxorubicin (DOX) and provide novel clinical treatment of gastric cancer, halloysite nanotubes (HNTs) loaded with DOX were encapsulated by soybean phospholipid (LIP) and the formed HNTs/DOX/LIP was systematically characterized via different techniques. The in vitro anticancer activity of HNTs/DOX/LIP was examined using an MTT assay. The antitumor efficacy and biocompatibility were monitored by measuring the tumor volume and assessing the blood routine and serum biochemistry using an ectopic implantation cancer model. The results show that when the concentration of HNTs was 3 mg/mL and the concentration of DOX was 1 mg/mL the optimal DOX loading efficiency was as high as 22.01%±0.43%. In vitro drug release behavior study demonstrated that HNTs/DOX/LIP shows a pH-responsive release property with fast drug release under acidic conditions (pH =5.4). MTT assays and in vivo experimental results revealed that HNTs/DOX/LIP exhibits a significantly higher inhibitory efficacy on the growth of mouse gastric cancer cells than free DOX at the same drug concentration. In addition, the life span of tumor-bearing mice in the HNTs/DOX/LIP-treated group was obviously prolonged compared with the control groups. Moreover, HNTs/DOX/LIP possessed excellent hemocompatibility as shown in the blood and histology studies. These findings indicated that the formed HNTs/DOX/LIP possesses higher antitumor efficacy and may be used as a targeted delivery nanoplatform for targeting therapy of different types of cancer cells. Keywords: HNTs, DOX, gastric cancer, drug carrierLi KZhang YChen MHu YJiang WZhou LLi SXu MZhao QWan RDove Medical PressarticleHNTsDOXgastric cancerdrug carrierMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 19-30 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	HNTs DOX gastric cancer drug carrier Medicine (General) R5-920
spellingShingle	HNTs DOX gastric cancer drug carrier Medicine (General) R5-920 Li K Zhang Y Chen M Hu Y Jiang W Zhou L Li S Xu M Zhao Q Wan R Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
description	Kai Li,1,* Yongxing Zhang,2,* Mengting Chen,1 Yangyang Hu,1 Weiliang Jiang,1 Li Zhou,1 Sisi Li,1 Min Xu,1 Qinghua Zhao,2 Rong Wan1 1Department of Gastroenterology, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 2Department of Orthopaedics, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: To improve the antitumor efficacy of doxorubicin (DOX) and provide novel clinical treatment of gastric cancer, halloysite nanotubes (HNTs) loaded with DOX were encapsulated by soybean phospholipid (LIP) and the formed HNTs/DOX/LIP was systematically characterized via different techniques. The in vitro anticancer activity of HNTs/DOX/LIP was examined using an MTT assay. The antitumor efficacy and biocompatibility were monitored by measuring the tumor volume and assessing the blood routine and serum biochemistry using an ectopic implantation cancer model. The results show that when the concentration of HNTs was 3 mg/mL and the concentration of DOX was 1 mg/mL the optimal DOX loading efficiency was as high as 22.01%±0.43%. In vitro drug release behavior study demonstrated that HNTs/DOX/LIP shows a pH-responsive release property with fast drug release under acidic conditions (pH =5.4). MTT assays and in vivo experimental results revealed that HNTs/DOX/LIP exhibits a significantly higher inhibitory efficacy on the growth of mouse gastric cancer cells than free DOX at the same drug concentration. In addition, the life span of tumor-bearing mice in the HNTs/DOX/LIP-treated group was obviously prolonged compared with the control groups. Moreover, HNTs/DOX/LIP possessed excellent hemocompatibility as shown in the blood and histology studies. These findings indicated that the formed HNTs/DOX/LIP possesses higher antitumor efficacy and may be used as a targeted delivery nanoplatform for targeting therapy of different types of cancer cells. Keywords: HNTs, DOX, gastric cancer, drug carrier
format	article
author	Li K Zhang Y Chen M Hu Y Jiang W Zhou L Li S Xu M Zhao Q Wan R
author_facet	Li K Zhang Y Chen M Hu Y Jiang W Zhou L Li S Xu M Zhao Q Wan R
author_sort	Li K
title	Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
title_short	Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
title_full	Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
title_fullStr	Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
title_full_unstemmed	Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
title_sort	enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/29d0cbe9b31c4a709317f37b29833724
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Enhanced antitumor efficacy of doxorubicin-encapsulated halloysite nanotubes

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