Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants

Plants have a remarkable regenerative capacity, which allows them to survive tissue damage after biotic and abiotic stresses. In this study, we use <i>Solanum lycopersicum</i> ‘Micro-Tom’ explants as a model to investigate wound-induced de novo organ formation, as these explants can rege...

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Autores principales:	Eduardo Larriba, Ana Belén Sánchez-García, María Salud Justamante, Cristina Martínez-Andújar, Alfonso Albacete, José Manuel Pérez-Pérez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	de novo shoot apical meristem regeneration de novo root regeneration time course RNA-Seq hormone balance U-HPLC-HRMS Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/29d14f5240e54955a526458ff22d403a
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spelling	oai:doaj.org-article:29d14f5240e54955a526458ff22d403a2021-11-11T17:16:24ZDynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants10.3390/ijms2221118431422-00671661-6596https://doaj.org/article/29d14f5240e54955a526458ff22d403a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11843https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Plants have a remarkable regenerative capacity, which allows them to survive tissue damage after biotic and abiotic stresses. In this study, we use <i>Solanum lycopersicum</i> ‘Micro-Tom’ explants as a model to investigate wound-induced de novo organ formation, as these explants can regenerate the missing structures without the exogenous application of plant hormones. Here, we performed simultaneous targeted profiling of 22 phytohormone-related metabolites during de novo organ formation and found that endogenous hormone levels dynamically changed after root and shoot excision, according to region-specific patterns. Our results indicate that a defined temporal window of high auxin-to-cytokinin accumulation in the basal region of the explants was required for adventitious root formation and that was dependent on a concerted regulation of polar auxin transport through the hypocotyl, of local induction of auxin biosynthesis, and of local inhibition of auxin degradation. In the apical region, though, a minimum of auxin-to-cytokinin ratio is established shortly after wounding both by decreasing active auxin levels and by draining auxin via its basipetal transport and internalization. Cross-validation with transcriptomic data highlighted the main hormonal gradients involved in wound-induced de novo organ formation in tomato hypocotyl explants.Eduardo LarribaAna Belén Sánchez-GarcíaMaría Salud JustamanteCristina Martínez-AndújarAlfonso AlbaceteJosé Manuel Pérez-PérezMDPI AGarticlede novo shoot apical meristem regenerationde novo root regenerationtime course RNA-Seqhormone balanceU-HPLC-HRMSBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11843, p 11843 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	de novo shoot apical meristem regeneration de novo root regeneration time course RNA-Seq hormone balance U-HPLC-HRMS Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	de novo shoot apical meristem regeneration de novo root regeneration time course RNA-Seq hormone balance U-HPLC-HRMS Biology (General) QH301-705.5 Chemistry QD1-999 Eduardo Larriba Ana Belén Sánchez-García María Salud Justamante Cristina Martínez-Andújar Alfonso Albacete José Manuel Pérez-Pérez Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
description	Plants have a remarkable regenerative capacity, which allows them to survive tissue damage after biotic and abiotic stresses. In this study, we use <i>Solanum lycopersicum</i> ‘Micro-Tom’ explants as a model to investigate wound-induced de novo organ formation, as these explants can regenerate the missing structures without the exogenous application of plant hormones. Here, we performed simultaneous targeted profiling of 22 phytohormone-related metabolites during de novo organ formation and found that endogenous hormone levels dynamically changed after root and shoot excision, according to region-specific patterns. Our results indicate that a defined temporal window of high auxin-to-cytokinin accumulation in the basal region of the explants was required for adventitious root formation and that was dependent on a concerted regulation of polar auxin transport through the hypocotyl, of local induction of auxin biosynthesis, and of local inhibition of auxin degradation. In the apical region, though, a minimum of auxin-to-cytokinin ratio is established shortly after wounding both by decreasing active auxin levels and by draining auxin via its basipetal transport and internalization. Cross-validation with transcriptomic data highlighted the main hormonal gradients involved in wound-induced de novo organ formation in tomato hypocotyl explants.
format	article
author	Eduardo Larriba Ana Belén Sánchez-García María Salud Justamante Cristina Martínez-Andújar Alfonso Albacete José Manuel Pérez-Pérez
author_facet	Eduardo Larriba Ana Belén Sánchez-García María Salud Justamante Cristina Martínez-Andújar Alfonso Albacete José Manuel Pérez-Pérez
author_sort	Eduardo Larriba
title	Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
title_short	Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
title_full	Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
title_fullStr	Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
title_full_unstemmed	Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants
title_sort	dynamic hormone gradients regulate wound-induced de novo organ formation in tomato hypocotyl explants
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/29d14f5240e54955a526458ff22d403a
work_keys_str_mv	AT eduardolarriba dynamichormonegradientsregulatewoundinduceddenovoorganformationintomatohypocotylexplants AT anabelensanchezgarcia dynamichormonegradientsregulatewoundinduceddenovoorganformationintomatohypocotylexplants AT mariasaludjustamante dynamichormonegradientsregulatewoundinduceddenovoorganformationintomatohypocotylexplants AT cristinamartinezandujar dynamichormonegradientsregulatewoundinduceddenovoorganformationintomatohypocotylexplants AT alfonsoalbacete dynamichormonegradientsregulatewoundinduceddenovoorganformationintomatohypocotylexplants AT josemanuelperezperez dynamichormonegradientsregulatewoundinduceddenovoorganformationintomatohypocotylexplants
_version_	1718432108025741312

Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants

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