From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>

The central dogma of biology, which explains how the information in genes flows into proteins, can be challenging to teach in the undergraduate classroom. To allow students to understand the correlation between genes and protein functions, we have developed a practical laboratory activity that compl...

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Autores principales:	Manuela Tripepi, Morgan Clear, Jack Lyons Bondi, Elvira Brunelli, Arianna Berardi
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/29d25124d2aa470585610cac8a0ebe88
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spelling	oai:doaj.org-article:29d25124d2aa470585610cac8a0ebe882021-11-15T15:04:32ZFrom Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>10.1128/jmbe.v21i2.21311935-78851935-7877https://doaj.org/article/29d25124d2aa470585610cac8a0ebe882020-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v21i2.2131https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885The central dogma of biology, which explains how the information in genes flows into proteins, can be challenging to teach in the undergraduate classroom. To allow students to understand the correlation between genes and protein functions, we have developed a practical laboratory activity that complements classroom learning. By using PCR analysis targeting the flagellin gene flgA1, students will investigate the genotype of an unknown strain of Haloferax volcanii, a halophilic archaeon appropriate for activities with undergraduates as it is nonpathogenic, inexpensive, easy to grow using “grocery store ingredients,” and offers many genetic tools. This species swims by means of flagella, and its motility can be tested using modified agar plates. Motile colonies will form swimming halos; meanwhile motility mutants will show only a dot on the motility plate, at the point of inoculation. First students will extract DNA for PCR amplification. They will not be told whether the strain they are assigned is motile or not. They will learn how to design primers for the target gene and set up the PCR reaction. Subsequently they will set up a motility assay and control of the provided strain. In the next laboratory period, students perform gel electrophoresis on the products of their PCR reactions and analyze the results. They compare these to the results of the motility assay to confirm the motility of the strain. In summary, this inquiry-based lab is easy and safe to perform and allows students to follow the information flow from a gene to a protein product.Manuela TripepiMorgan ClearJack Lyons BondiElvira BrunelliArianna BerardiAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Manuela Tripepi Morgan Clear Jack Lyons Bondi Elvira Brunelli Arianna Berardi From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
description	The central dogma of biology, which explains how the information in genes flows into proteins, can be challenging to teach in the undergraduate classroom. To allow students to understand the correlation between genes and protein functions, we have developed a practical laboratory activity that complements classroom learning. By using PCR analysis targeting the flagellin gene flgA1, students will investigate the genotype of an unknown strain of Haloferax volcanii, a halophilic archaeon appropriate for activities with undergraduates as it is nonpathogenic, inexpensive, easy to grow using “grocery store ingredients,” and offers many genetic tools. This species swims by means of flagella, and its motility can be tested using modified agar plates. Motile colonies will form swimming halos; meanwhile motility mutants will show only a dot on the motility plate, at the point of inoculation. First students will extract DNA for PCR amplification. They will not be told whether the strain they are assigned is motile or not. They will learn how to design primers for the target gene and set up the PCR reaction. Subsequently they will set up a motility assay and control of the provided strain. In the next laboratory period, students perform gel electrophoresis on the products of their PCR reactions and analyze the results. They compare these to the results of the motility assay to confirm the motility of the strain. In summary, this inquiry-based lab is easy and safe to perform and allows students to follow the information flow from a gene to a protein product.
format	article
author	Manuela Tripepi Morgan Clear Jack Lyons Bondi Elvira Brunelli Arianna Berardi
author_facet	Manuela Tripepi Morgan Clear Jack Lyons Bondi Elvira Brunelli Arianna Berardi
author_sort	Manuela Tripepi
title	From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
title_short	From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
title_full	From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
title_fullStr	From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
title_full_unstemmed	From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
title_sort	from gene to function: a safe laboratory exercise promoting the learning of the central dogma of biology for the undergraduate biology curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/29d25124d2aa470585610cac8a0ebe88
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From Gene to Function: A Safe Laboratory Exercise Promoting the Learning of the Central Dogma of Biology for the Undergraduate Biology Curriculum<xref ref-type="fn" rid="fn1-jmbe-21-61">†</xref>

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