Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites

Although the varietal diversity is fairly rich in guava, most varieties lack one or more desirable characters. Hence, attempts were made for improving specific traits, viz., attractive pink pulp colour, soft seeds, medium fruit size, high TSS and high ascorbic acid. Genetic diversity analysis is a p...

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Autores principales:	M V Naga Chaithanya, M R Dinesh, C Vasugi, D C Lakshmana Reddy, D Sailaja, C Aswath
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2014
Materias:	guava genetic diversity dendrogram simple sequence repeats Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/29dc2b49a29d4f1aa1d98d4b35ffff66
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spelling	oai:doaj.org-article:29dc2b49a29d4f1aa1d98d4b35ffff662021-12-02T10:35:44ZAssessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites0973-354X2582-4899https://doaj.org/article/29dc2b49a29d4f1aa1d98d4b35ffff662014-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/180https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Although the varietal diversity is fairly rich in guava, most varieties lack one or more desirable characters. Hence, attempts were made for improving specific traits, viz., attractive pink pulp colour, soft seeds, medium fruit size, high TSS and high ascorbic acid. Genetic diversity analysis is a prerequisite for identifying potential parents in breeding programs and germplasm conservation. Molecular characterization helps discriminate closely-related genotypes, as, this technique is unaffected by environment, rendering it more reliable. In this study, 48 polymorphic SSRs screened from a total of 115 SSR markers were used for analyzing marker segregation in 72 guava accessions. Statistical analysis was done using IDENTITY1.0 and CERVUS 3.0 software. Cluster analysis was done with DARwin 5.0 software, using Wards Minimum Variance method, and weighted group neighbour joining method, to check reliability of grouping among clusters. The trend in grouping was found to be similar in both methods. Dendrograms generated showed that the hybrids clustered with their parents; exotic collections fell into two different sub-groups based on productivity; the wild species formed one group; and Navalar cultivars from Dharwad clustered together, reflecting similar origin.M V Naga ChaithanyaM R DineshC VasugiD C Lakshmana ReddyD SailajaC AswathSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleguavagenetic diversitydendrogramsimple sequence repeatsPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 9, Iss 2, Pp 117-125 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	guava genetic diversity dendrogram simple sequence repeats Plant culture SB1-1110
spellingShingle	guava genetic diversity dendrogram simple sequence repeats Plant culture SB1-1110 M V Naga Chaithanya M R Dinesh C Vasugi D C Lakshmana Reddy D Sailaja C Aswath Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
description	Although the varietal diversity is fairly rich in guava, most varieties lack one or more desirable characters. Hence, attempts were made for improving specific traits, viz., attractive pink pulp colour, soft seeds, medium fruit size, high TSS and high ascorbic acid. Genetic diversity analysis is a prerequisite for identifying potential parents in breeding programs and germplasm conservation. Molecular characterization helps discriminate closely-related genotypes, as, this technique is unaffected by environment, rendering it more reliable. In this study, 48 polymorphic SSRs screened from a total of 115 SSR markers were used for analyzing marker segregation in 72 guava accessions. Statistical analysis was done using IDENTITY1.0 and CERVUS 3.0 software. Cluster analysis was done with DARwin 5.0 software, using Wards Minimum Variance method, and weighted group neighbour joining method, to check reliability of grouping among clusters. The trend in grouping was found to be similar in both methods. Dendrograms generated showed that the hybrids clustered with their parents; exotic collections fell into two different sub-groups based on productivity; the wild species formed one group; and Navalar cultivars from Dharwad clustered together, reflecting similar origin.
format	article
author	M V Naga Chaithanya M R Dinesh C Vasugi D C Lakshmana Reddy D Sailaja C Aswath
author_facet	M V Naga Chaithanya M R Dinesh C Vasugi D C Lakshmana Reddy D Sailaja C Aswath
author_sort	M V Naga Chaithanya
title	Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
title_short	Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
title_full	Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
title_fullStr	Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
title_full_unstemmed	Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
title_sort	assessment of genetic diversity in guava (psidium guajava) germplasm using microsatellites
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2014
url	https://doaj.org/article/29dc2b49a29d4f1aa1d98d4b35ffff66
work_keys_str_mv	AT mvnagachaithanya assessmentofgeneticdiversityinguavapsidiumguajavagermplasmusingmicrosatellites AT mrdinesh assessmentofgeneticdiversityinguavapsidiumguajavagermplasmusingmicrosatellites AT cvasugi assessmentofgeneticdiversityinguavapsidiumguajavagermplasmusingmicrosatellites AT dclakshmanareddy assessmentofgeneticdiversityinguavapsidiumguajavagermplasmusingmicrosatellites AT dsailaja assessmentofgeneticdiversityinguavapsidiumguajavagermplasmusingmicrosatellites AT caswath assessmentofgeneticdiversityinguavapsidiumguajavagermplasmusingmicrosatellites
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Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites

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