The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition

Abstract. Widiarso, Nurcahyo W, Kurniasih, Prastowo J. 2021. The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition. Biodiversitas 22: 1-5. The ultrastructural changes induced in adult Haemonchus contortus in vitro usi...

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Autores principales:	Budi Purwo Widiarso, WISNU NURCAHYO, KURNIASIH KURNIASIH, JOKO PRASTOWO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bamboo leaves, extract haemonchus contortus, in vitro, scanning electron microscopy, ultrastructure Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/29dc772c9d4d444ab7927118a69cb2cf
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spelling	oai:doaj.org-article:29dc772c9d4d444ab7927118a69cb2cf2021-11-22T00:53:57ZThe ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition1412-033X2085-472210.13057/biodiv/d220101https://doaj.org/article/29dc772c9d4d444ab7927118a69cb2cf2020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6911https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Widiarso, Nurcahyo W, Kurniasih, Prastowo J. 2021. The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition. Biodiversitas 22: 1-5. The ultrastructural changes induced in adult Haemonchus contortus in vitro using the aqueous extract of bamboo leaves were assessed using scanning electron microscopy (SEM). The H. contortus adult females were obtained from three groups and treatment was repeated thrice. The first group (T0) was not treated with bamboo leaves; 100% of the worms lived. The second group (T1), treated with 0.1% bamboo leaf-extract, had 50% mortality 4 h after examination. The third group (T2), treated with 1% bamboo leaf-extract, had 100% mortality 4 h after examination. Five worms used per treatment were submerged in ethanol and incubated for 24 hours. The ultrastructural changes observed by SEM revealed structural alteration of the worm surface after in vitro contact with the bamboo leaf aqueous extract and compared to the control worms. The main changes concerned the anterior end or cephalic region, cuticle surface, and vulva flap area. The structural modification of the external part of the female reproductive system was found only in vitro. The structural changes found in the worms exposed to the bamboo leaves might affect their motility and nutrition with possible consequences on their reproduction. Transmission electron microscopy may help to understand the external changes observed in H. contortus.Budi Purwo WidiarsoWISNU NURCAHYOKURNIASIH KURNIASIHJOKO PRASTOWOMBI & UNS Soloarticlebamboo leaves, extract haemonchus contortus, in vitro, scanning electron microscopy, ultrastructureBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bamboo leaves, extract haemonchus contortus, in vitro, scanning electron microscopy, ultrastructure Biology (General) QH301-705.5
spellingShingle	bamboo leaves, extract haemonchus contortus, in vitro, scanning electron microscopy, ultrastructure Biology (General) QH301-705.5 Budi Purwo Widiarso WISNU NURCAHYO KURNIASIH KURNIASIH JOKO PRASTOWO The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition
description	Abstract. Widiarso, Nurcahyo W, Kurniasih, Prastowo J. 2021. The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition. Biodiversitas 22: 1-5. The ultrastructural changes induced in adult Haemonchus contortus in vitro using the aqueous extract of bamboo leaves were assessed using scanning electron microscopy (SEM). The H. contortus adult females were obtained from three groups and treatment was repeated thrice. The first group (T0) was not treated with bamboo leaves; 100% of the worms lived. The second group (T1), treated with 0.1% bamboo leaf-extract, had 50% mortality 4 h after examination. The third group (T2), treated with 1% bamboo leaf-extract, had 100% mortality 4 h after examination. Five worms used per treatment were submerged in ethanol and incubated for 24 hours. The ultrastructural changes observed by SEM revealed structural alteration of the worm surface after in vitro contact with the bamboo leaf aqueous extract and compared to the control worms. The main changes concerned the anterior end or cephalic region, cuticle surface, and vulva flap area. The structural modification of the external part of the female reproductive system was found only in vitro. The structural changes found in the worms exposed to the bamboo leaves might affect their motility and nutrition with possible consequences on their reproduction. Transmission electron microscopy may help to understand the external changes observed in H. contortus.
format	article
author	Budi Purwo Widiarso WISNU NURCAHYO KURNIASIH KURNIASIH JOKO PRASTOWO
author_facet	Budi Purwo Widiarso WISNU NURCAHYO KURNIASIH KURNIASIH JOKO PRASTOWO
author_sort	Budi Purwo Widiarso
title	The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition
title_short	The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition
title_full	The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition
title_fullStr	The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition
title_full_unstemmed	The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition
title_sort	ultrastructure changes of haemonchus contortus exposed to bamboo leaves (gigantochloa apus) aqueous extract under in vitro condition
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/29dc772c9d4d444ab7927118a69cb2cf
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The ultrastructure changes of Haemonchus contortus exposed to bamboo leaves (Gigantochloa apus) aqueous extract under in vitro condition

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