High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran

Acanthamoeba spp. is a free-living amoeba that can cause major infections in humans, including keratitis and granulomatous encephalitis. Thus, water resources play an important role in transmitting Acanthamoeba spp. infection to humans. The purpose of this study was to investigate the presence of Ac...

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Autores principales:	Raheleh Eftekhari-Kenzerki, Kavous Solhjoo, Zahra Babaei, Hassan Rezanezhad, Ahmad Abolghazi, Ali Taghipour
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	acanthamoeba iran swimming pool Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/29dfbd2ccfbc4029a146b991e72c26bd
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spelling	oai:doaj.org-article:29dfbd2ccfbc4029a146b991e72c26bd2021-11-06T06:31:09ZHigh occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran1477-89201996-782910.2166/wh.2021.162https://doaj.org/article/29dfbd2ccfbc4029a146b991e72c26bd2021-10-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/5/864https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Acanthamoeba spp. is a free-living amoeba that can cause major infections in humans, including keratitis and granulomatous encephalitis. Thus, water resources play an important role in transmitting Acanthamoeba spp. infection to humans. The purpose of this study was to investigate the presence of Acanthamoeba spp. in public swimming pools from three cities of Kerman Province, southeastern Iran. Eighty water samples of 20 public indoor swimming pools were taken from Kerman, Jiroft, and Kahnauj cities. Water temperature (°C), pH, and free chlorine concentration (ppm) were measured. Filtration and cultivation were applied on non-nutrient agar medium. The polymerase chain reaction was applied by using the genus-specific primers (JDP1 and JDP2) on positive samples; these primers can amplify the 423–551 bp fragment. Eighteen of the 20 swimming pools (including 32/80; 40% samples) were contaminated with Acanthamoeba spp. All swimming pools of Jiroft and Kahnauj and 88.2% of swimming pools in Kerman were contaminated. As such, all 32 Acanthamoeba isolates were amplified using the JDP primer pairs. Two genotypes, T3 and T4, were also identified. The present research is the first to report Acanthamoeba spp. in public swimming pools from Kerman Province. Due to high occurrence of this protozoan, it is recommended to use warning signs around swimming pools to create awareness of this infection. HIGHLIGHTS This is the first study on the occurrence of Acanthamoeba spp. in public swimming pools from Kerman Province.; Eighteen of the 20 swimming pools (including 32/80; 40% samples) were contaminated with Acanthamoeba spp.; It is recommended to use warning signs around swimming pools to raise awareness of this infection.;Raheleh Eftekhari-KenzerkiKavous SolhjooZahra BabaeiHassan RezanezhadAhmad AbolghaziAli TaghipourIWA Publishingarticleacanthamoebairanswimming poolPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 5, Pp 864-871 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acanthamoeba iran swimming pool Public aspects of medicine RA1-1270
spellingShingle	acanthamoeba iran swimming pool Public aspects of medicine RA1-1270 Raheleh Eftekhari-Kenzerki Kavous Solhjoo Zahra Babaei Hassan Rezanezhad Ahmad Abolghazi Ali Taghipour High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
description	Acanthamoeba spp. is a free-living amoeba that can cause major infections in humans, including keratitis and granulomatous encephalitis. Thus, water resources play an important role in transmitting Acanthamoeba spp. infection to humans. The purpose of this study was to investigate the presence of Acanthamoeba spp. in public swimming pools from three cities of Kerman Province, southeastern Iran. Eighty water samples of 20 public indoor swimming pools were taken from Kerman, Jiroft, and Kahnauj cities. Water temperature (°C), pH, and free chlorine concentration (ppm) were measured. Filtration and cultivation were applied on non-nutrient agar medium. The polymerase chain reaction was applied by using the genus-specific primers (JDP1 and JDP2) on positive samples; these primers can amplify the 423–551 bp fragment. Eighteen of the 20 swimming pools (including 32/80; 40% samples) were contaminated with Acanthamoeba spp. All swimming pools of Jiroft and Kahnauj and 88.2% of swimming pools in Kerman were contaminated. As such, all 32 Acanthamoeba isolates were amplified using the JDP primer pairs. Two genotypes, T3 and T4, were also identified. The present research is the first to report Acanthamoeba spp. in public swimming pools from Kerman Province. Due to high occurrence of this protozoan, it is recommended to use warning signs around swimming pools to create awareness of this infection. HIGHLIGHTS This is the first study on the occurrence of Acanthamoeba spp. in public swimming pools from Kerman Province.; Eighteen of the 20 swimming pools (including 32/80; 40% samples) were contaminated with Acanthamoeba spp.; It is recommended to use warning signs around swimming pools to raise awareness of this infection.;
format	article
author	Raheleh Eftekhari-Kenzerki Kavous Solhjoo Zahra Babaei Hassan Rezanezhad Ahmad Abolghazi Ali Taghipour
author_facet	Raheleh Eftekhari-Kenzerki Kavous Solhjoo Zahra Babaei Hassan Rezanezhad Ahmad Abolghazi Ali Taghipour
author_sort	Raheleh Eftekhari-Kenzerki
title	High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
title_short	High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
title_full	High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
title_fullStr	High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
title_full_unstemmed	High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran
title_sort	high occurrence of acanthamoeba spp. in the water samples of public swimming pools from kerman province, iran
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/29dfbd2ccfbc4029a146b991e72c26bd
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High occurrence of Acanthamoeba spp. in the water samples of public swimming pools from Kerman Province, Iran

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