Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients

Abstract The most widely used test for the diagnosis of SARS-CoV-2 infection is a PCR test. PCR has very high sensitivity and is able to detect very low amounts of RNA. However, many individuals receiving a positive test result in a context of a PCR-based surveillance might be infected with SARS-CoV...

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Autores principales:	John G. Routsias, Maria Mavrouli, Panagiota Tsoplou, Kyriaki Dioikitopoulou, Athanasios Tsakris
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29f51de075694e1f911e7d37cfea0b62
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spelling	oai:doaj.org-article:29f51de075694e1f911e7d37cfea0b622021-11-28T12:18:02ZDiagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients10.1038/s41598-021-02197-z2045-2322https://doaj.org/article/29f51de075694e1f911e7d37cfea0b622021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02197-zhttps://doaj.org/toc/2045-2322Abstract The most widely used test for the diagnosis of SARS-CoV-2 infection is a PCR test. PCR has very high sensitivity and is able to detect very low amounts of RNA. However, many individuals receiving a positive test result in a context of a PCR-based surveillance might be infected with SARS-CoV-2, but they are not contagious at the time of the test. The question arises regards if the cost effective, portable rapid antigen tests (RATs) have a better performance than PCR in identification of infectious individuals. In this direction, we examined the diagnostic performance of RATs from 14 different manufacturers in 400 clinical samples with known rRT-PCR cycles threshold (cT) and 50 control samples. Substantial variability was observed in the limit of detection (LOD) of different RATs (cT = 26.8–34.7). The fluorescence-based RAT exhibited a LOD of cT = 34.7. The use of the most effective RATs leads to true positive rates (sensitivities) of 99.1% and 90.9% for samples with cT ≤ 30 and cT ≤ 33, respectively, percentages that can guarantee a sensitivity high enough to identify contagious patients. RAT testing may also substantially reduce the quarantine period for infected individuals without compromising personal or public safety.John G. RoutsiasMaria MavrouliPanagiota TsoplouKyriaki DioikitopoulouAthanasios TsakrisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John G. Routsias Maria Mavrouli Panagiota Tsoplou Kyriaki Dioikitopoulou Athanasios Tsakris Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients
description	Abstract The most widely used test for the diagnosis of SARS-CoV-2 infection is a PCR test. PCR has very high sensitivity and is able to detect very low amounts of RNA. However, many individuals receiving a positive test result in a context of a PCR-based surveillance might be infected with SARS-CoV-2, but they are not contagious at the time of the test. The question arises regards if the cost effective, portable rapid antigen tests (RATs) have a better performance than PCR in identification of infectious individuals. In this direction, we examined the diagnostic performance of RATs from 14 different manufacturers in 400 clinical samples with known rRT-PCR cycles threshold (cT) and 50 control samples. Substantial variability was observed in the limit of detection (LOD) of different RATs (cT = 26.8–34.7). The fluorescence-based RAT exhibited a LOD of cT = 34.7. The use of the most effective RATs leads to true positive rates (sensitivities) of 99.1% and 90.9% for samples with cT ≤ 30 and cT ≤ 33, respectively, percentages that can guarantee a sensitivity high enough to identify contagious patients. RAT testing may also substantially reduce the quarantine period for infected individuals without compromising personal or public safety.
format	article
author	John G. Routsias Maria Mavrouli Panagiota Tsoplou Kyriaki Dioikitopoulou Athanasios Tsakris
author_facet	John G. Routsias Maria Mavrouli Panagiota Tsoplou Kyriaki Dioikitopoulou Athanasios Tsakris
author_sort	John G. Routsias
title	Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients
title_short	Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients
title_full	Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients
title_fullStr	Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients
title_full_unstemmed	Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients
title_sort	diagnostic performance of rapid antigen tests (rats) for sars-cov-2 and their efficacy in monitoring the infectiousness of covid-19 patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/29f51de075694e1f911e7d37cfea0b62
work_keys_str_mv	AT johngroutsias diagnosticperformanceofrapidantigentestsratsforsarscov2andtheirefficacyinmonitoringtheinfectiousnessofcovid19patients AT mariamavrouli diagnosticperformanceofrapidantigentestsratsforsarscov2andtheirefficacyinmonitoringtheinfectiousnessofcovid19patients AT panagiotatsoplou diagnosticperformanceofrapidantigentestsratsforsarscov2andtheirefficacyinmonitoringtheinfectiousnessofcovid19patients AT kyriakidioikitopoulou diagnosticperformanceofrapidantigentestsratsforsarscov2andtheirefficacyinmonitoringtheinfectiousnessofcovid19patients AT athanasiostsakris diagnosticperformanceofrapidantigentestsratsforsarscov2andtheirefficacyinmonitoringtheinfectiousnessofcovid19patients
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Diagnostic performance of rapid antigen tests (RATs) for SARS-CoV-2 and their efficacy in monitoring the infectiousness of COVID-19 patients

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