Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis

Abstract Condensin complexes play crucial roles in chromosome condensation that is a fundamental process to establish the “rod-like” shape of chromosome structure in mitosis. Failure of the chromosome assembly causes chromosome segregation errors and subsequent genomic instability. However, a molecu...

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Autores principales:	Yuya Kagami, Masaya Ono, Kiyotsugu Yoshida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29f53593874544778c63f94355e4607c
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spelling	oai:doaj.org-article:29f53593874544778c63f94355e4607c2021-12-02T11:41:21ZPlk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis10.1038/s41598-017-05986-72045-2322https://doaj.org/article/29f53593874544778c63f94355e4607c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05986-7https://doaj.org/toc/2045-2322Abstract Condensin complexes play crucial roles in chromosome condensation that is a fundamental process to establish the “rod-like” shape of chromosome structure in mitosis. Failure of the chromosome assembly causes chromosome segregation errors and subsequent genomic instability. However, a molecular mechanism that controls condensin function for the chromosomal organization has not been fully understood. Here, we show that the abundance of CAP-H2, one of the condensin II subunits, is fluctuated during the cell cycle in accordance with Plk1 kinase activity. Inhibition of Plk1 leads to Cdc20-mediated degradation of CAP-H2 in mitosis. Plk1 phosphorylation of CAP-H2 at Ser288 is required for the accumulation of CAP-H2 and accurate chromosomal condensation during prophase. These findings suggest that Plk1 phosphorylation regulates condensin II function by modulating CAP-H2 expression levels to facilitate proper mitotic chromosome organization.Yuya KagamiMasaya OnoKiyotsugu YoshidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuya Kagami Masaya Ono Kiyotsugu Yoshida Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
description	Abstract Condensin complexes play crucial roles in chromosome condensation that is a fundamental process to establish the “rod-like” shape of chromosome structure in mitosis. Failure of the chromosome assembly causes chromosome segregation errors and subsequent genomic instability. However, a molecular mechanism that controls condensin function for the chromosomal organization has not been fully understood. Here, we show that the abundance of CAP-H2, one of the condensin II subunits, is fluctuated during the cell cycle in accordance with Plk1 kinase activity. Inhibition of Plk1 leads to Cdc20-mediated degradation of CAP-H2 in mitosis. Plk1 phosphorylation of CAP-H2 at Ser288 is required for the accumulation of CAP-H2 and accurate chromosomal condensation during prophase. These findings suggest that Plk1 phosphorylation regulates condensin II function by modulating CAP-H2 expression levels to facilitate proper mitotic chromosome organization.
format	article
author	Yuya Kagami Masaya Ono Kiyotsugu Yoshida
author_facet	Yuya Kagami Masaya Ono Kiyotsugu Yoshida
author_sort	Yuya Kagami
title	Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
title_short	Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
title_full	Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
title_fullStr	Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
title_full_unstemmed	Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis
title_sort	plk1 phosphorylation of cap-h2 triggers chromosome condensation by condensin ii at the early phase of mitosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/29f53593874544778c63f94355e4607c
work_keys_str_mv	AT yuyakagami plk1phosphorylationofcaph2triggerschromosomecondensationbycondensiniiattheearlyphaseofmitosis AT masayaono plk1phosphorylationofcaph2triggerschromosomecondensationbycondensiniiattheearlyphaseofmitosis AT kiyotsuguyoshida plk1phosphorylationofcaph2triggerschromosomecondensationbycondensiniiattheearlyphaseofmitosis
_version_	1718395389750542336

Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosis

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