Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.

Neonatal rat primary myocardial cells were subjected to heat stress in vitro, as a model for investigating the distribution and expression of Hsp27 and αB-crystallin. After exposure to heat stress at 42°C for different durations, the activities of enzymes expressed during cell damage increased in th...

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Autores principales:	Shu Tang, Rehana Buriro, Zhijun Liu, Miao Zhang, Islam Ali, Abdelnasir Adam, Jörg Hartung, Endong Bao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29fef0291664417a9a27fafeff2ea17f
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spelling	oai:doaj.org-article:29fef0291664417a9a27fafeff2ea17f2021-11-18T09:03:48ZLocalization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.1932-620310.1371/journal.pone.0069066https://doaj.org/article/29fef0291664417a9a27fafeff2ea17f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23894407/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Neonatal rat primary myocardial cells were subjected to heat stress in vitro, as a model for investigating the distribution and expression of Hsp27 and αB-crystallin. After exposure to heat stress at 42°C for different durations, the activities of enzymes expressed during cell damage increased in the supernatant of the heat-stressed myocardial cells from 10 min, and the pathological lesions were characterized by karyopyknosis and acute degeneration. Thus, cell damage was induced at the onset of heat stress. Immunofluorescence analysis showed stronger positive signals for both Hsp27 and αB-crystallin from 10 min to 240 min of exposure compared to the control cells. According to the Western blotting results, during the 480 min of heat stress, no significant variation was found in Hsp27 and αB-crystallin expression; however, significant differences were found in the induction of their corresponding mRNAs. The expression of these small heat shock proteins (sHsps) was probably delayed or overtaxed due to the rapid consumption of sHsps in myocardial cells at the onset of heat stress. Our findings indicate that Hsp27 and αB-crystallin do play a role in the response of cardiac cells to heat stress, but the details of their function remain to be investigated.Shu TangRehana BuriroZhijun LiuMiao ZhangIslam AliAbdelnasir AdamJörg HartungEndong BaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69066 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shu Tang Rehana Buriro Zhijun Liu Miao Zhang Islam Ali Abdelnasir Adam Jörg Hartung Endong Bao Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
description	Neonatal rat primary myocardial cells were subjected to heat stress in vitro, as a model for investigating the distribution and expression of Hsp27 and αB-crystallin. After exposure to heat stress at 42°C for different durations, the activities of enzymes expressed during cell damage increased in the supernatant of the heat-stressed myocardial cells from 10 min, and the pathological lesions were characterized by karyopyknosis and acute degeneration. Thus, cell damage was induced at the onset of heat stress. Immunofluorescence analysis showed stronger positive signals for both Hsp27 and αB-crystallin from 10 min to 240 min of exposure compared to the control cells. According to the Western blotting results, during the 480 min of heat stress, no significant variation was found in Hsp27 and αB-crystallin expression; however, significant differences were found in the induction of their corresponding mRNAs. The expression of these small heat shock proteins (sHsps) was probably delayed or overtaxed due to the rapid consumption of sHsps in myocardial cells at the onset of heat stress. Our findings indicate that Hsp27 and αB-crystallin do play a role in the response of cardiac cells to heat stress, but the details of their function remain to be investigated.
format	article
author	Shu Tang Rehana Buriro Zhijun Liu Miao Zhang Islam Ali Abdelnasir Adam Jörg Hartung Endong Bao
author_facet	Shu Tang Rehana Buriro Zhijun Liu Miao Zhang Islam Ali Abdelnasir Adam Jörg Hartung Endong Bao
author_sort	Shu Tang
title	Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
title_short	Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
title_full	Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
title_fullStr	Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
title_full_unstemmed	Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.
title_sort	localization and expression of hsp27 and αb-crystallin in rat primary myocardial cells during heat stress in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/29fef0291664417a9a27fafeff2ea17f
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Localization and expression of Hsp27 and αB-crystallin in rat primary myocardial cells during heat stress in vitro.

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