Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration

Chang-Moon Lee,1 Tai Kyoung Lee,2–5 Dae-Ik Kim,1,6 Yu-Ri Kim,7 Meyoung-Kon Kim,7 Hwan-Jeong Jeong,2–5 Myung-Hee Sohn,2–5 Seok Tae Lim2–5 1Department of Biomedical Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea; 2Department of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lee CM, Lee TK, Kim DI, Kim YR, Kim MK, Jeong HJ, Sohn MH, Lim ST
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2a03daa737334a2bbe1e142ee5c2e8fc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a03daa737334a2bbe1e142ee5c2e8fc
record_format	dspace
spelling	oai:doaj.org-article:2a03daa737334a2bbe1e142ee5c2e8fc2021-12-02T08:08:04ZOptical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration1178-2013https://doaj.org/article/2a03daa737334a2bbe1e142ee5c2e8fc2014-12-01T00:00:00Zhttp://www.dovepress.com/optical-imaging-of-absorption-and-distribution-of-ritc-sio2-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Chang-Moon Lee,1 Tai Kyoung Lee,2–5 Dae-Ik Kim,1,6 Yu-Ri Kim,7 Meyoung-Kon Kim,7 Hwan-Jeong Jeong,2–5 Myung-Hee Sohn,2–5 Seok Tae Lim2–5 1Department of Biomedical Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea; 2Department of Nuclear Medicine, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 3Cyclotron Research Center, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 4Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 5Molecular Imaging and Therapeutic Medicine Research Center, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 6School of Electrical, Electronic Communication, and Computer Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea; 7Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seounbuk-Gu, Seoul, Republic of Korea Purpose: In this study, we investigated the absorption and distribution of rhodamine B isothiocyanate (RITC)-incorporated silica oxide nanoparticles(SiNPs) (RITC-SiNPs) after oral exposure, by conducting optical imaging, with a focus on tracking the movement of RITC-SiNPs of different particle size and surface charge. Methods: RITC-SiNPs (20 or 100 nm; positively or negatively charged) were used to avoid the dissociation of a fluorescent dye from nanoparticles via spontaneous or enzyme-catalyzed reactions in vivo. The changes in the nanoparticle sizes and shapes were investigated in an HCl solution for 6 hours. RITC-SiNPs were orally administered to healthy nude mice at a dose of 100 mg/kg. Optical imaging studies were performed at 2, 4, and 6 hours after oral administration. The mice were sacrificed at 2, 4, 6, and 10 hours post-administration, and ex vivo imaging studies were performed. Results: The RITC-SiNPs were stable in the HCl solution for 6 hours, without dissociation of RITC from the nanoparticles and without changes in size and shape. RITC-SiNPs flowed into the small intestine from the stomach and gradually moved along the gut during the experiment. In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys. The orally administered RITC-SiNPs, which were absorbed in the systemic circulation, were eliminated from the body into the urine. The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs. The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible. Conclusion: We demonstrated that the movement of RITC-SiNPs after oral exposure could be traced by optical imaging. Optical imaging has the potential to provide valuable information that will help in understanding the behavior of SiNPs in the body following exposure. Keywords: silica nanoparticles, oral exposure, rhodamine B isothiocyanate, RITC-SiNPLee CMLee TKKim DIKim YRKim MKJeong HJSohn MHLim STDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Supplement 2, Pp 243-250 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lee CM Lee TK Kim DI Kim YR Kim MK Jeong HJ Sohn MH Lim ST Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration
description	Chang-Moon Lee,1 Tai Kyoung Lee,2–5 Dae-Ik Kim,1,6 Yu-Ri Kim,7 Meyoung-Kon Kim,7 Hwan-Jeong Jeong,2–5 Myung-Hee Sohn,2–5 Seok Tae Lim2–5 1Department of Biomedical Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea; 2Department of Nuclear Medicine, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 3Cyclotron Research Center, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 4Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 5Molecular Imaging and Therapeutic Medicine Research Center, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea; 6School of Electrical, Electronic Communication, and Computer Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea; 7Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seounbuk-Gu, Seoul, Republic of Korea Purpose: In this study, we investigated the absorption and distribution of rhodamine B isothiocyanate (RITC)-incorporated silica oxide nanoparticles(SiNPs) (RITC-SiNPs) after oral exposure, by conducting optical imaging, with a focus on tracking the movement of RITC-SiNPs of different particle size and surface charge. Methods: RITC-SiNPs (20 or 100 nm; positively or negatively charged) were used to avoid the dissociation of a fluorescent dye from nanoparticles via spontaneous or enzyme-catalyzed reactions in vivo. The changes in the nanoparticle sizes and shapes were investigated in an HCl solution for 6 hours. RITC-SiNPs were orally administered to healthy nude mice at a dose of 100 mg/kg. Optical imaging studies were performed at 2, 4, and 6 hours after oral administration. The mice were sacrificed at 2, 4, 6, and 10 hours post-administration, and ex vivo imaging studies were performed. Results: The RITC-SiNPs were stable in the HCl solution for 6 hours, without dissociation of RITC from the nanoparticles and without changes in size and shape. RITC-SiNPs flowed into the small intestine from the stomach and gradually moved along the gut during the experiment. In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys. The orally administered RITC-SiNPs, which were absorbed in the systemic circulation, were eliminated from the body into the urine. The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs. The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible. Conclusion: We demonstrated that the movement of RITC-SiNPs after oral exposure could be traced by optical imaging. Optical imaging has the potential to provide valuable information that will help in understanding the behavior of SiNPs in the body following exposure. Keywords: silica nanoparticles, oral exposure, rhodamine B isothiocyanate, RITC-SiNP
format	article
author	Lee CM Lee TK Kim DI Kim YR Kim MK Jeong HJ Sohn MH Lim ST
author_facet	Lee CM Lee TK Kim DI Kim YR Kim MK Jeong HJ Sohn MH Lim ST
author_sort	Lee CM
title	Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration
title_short	Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration
title_full	Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration
title_fullStr	Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration
title_full_unstemmed	Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration
title_sort	optical imaging of absorption and distribution of ritc-sio2 nanoparticles after oral administration
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/2a03daa737334a2bbe1e142ee5c2e8fc
work_keys_str_mv	AT leecm opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT leetk opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT kimdi opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT kimyr opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT kimmk opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT jeonghj opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT sohnmh opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration AT limst opticalimagingofabsorptionanddistributionofritcsio2nanoparticlesafteroraladministration
_version_	1718398592170852352

Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration

Ejemplares similares