Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis

Metal ions at the active site of an enzyme act as cofactors and their dynamic fluctuations might influence enzyme activity. Here authors use single-molecule FRET to study λ-exonuclease and find that metal-ion-coordination is correlated with enzymatic reaction-steps.

Guardado en:

Detalles Bibliográficos
Autores principales:	Wonseok Hwang, Jungmin Yoo, Yuno Lee, Suyeon Park, Phuong Lien Hoang, HyeokJin Cho, Jeongmin Yu, Thi Minh Hoa Vo, Minsang Shin, Mi Sun Jin, Daeho Park, Changbong Hyeon, Gwangrog Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2a0749b91eb14221840220e83adac0c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a0749b91eb14221840220e83adac0c3
record_format	dspace
spelling	oai:doaj.org-article:2a0749b91eb14221840220e83adac0c32021-12-02T16:49:52ZDynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis10.1038/s41467-018-06750-92041-1723https://doaj.org/article/2a0749b91eb14221840220e83adac0c32018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06750-9https://doaj.org/toc/2041-1723Metal ions at the active site of an enzyme act as cofactors and their dynamic fluctuations might influence enzyme activity. Here authors use single-molecule FRET to study λ-exonuclease and find that metal-ion-coordination is correlated with enzymatic reaction-steps.Wonseok HwangJungmin YooYuno LeeSuyeon ParkPhuong Lien HoangHyeokJin ChoJeongmin YuThi Minh Hoa VoMinsang ShinMi Sun JinDaeho ParkChangbong HyeonGwangrog LeeNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wonseok Hwang Jungmin Yoo Yuno Lee Suyeon Park Phuong Lien Hoang HyeokJin Cho Jeongmin Yu Thi Minh Hoa Vo Minsang Shin Mi Sun Jin Daeho Park Changbong Hyeon Gwangrog Lee Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
description	Metal ions at the active site of an enzyme act as cofactors and their dynamic fluctuations might influence enzyme activity. Here authors use single-molecule FRET to study λ-exonuclease and find that metal-ion-coordination is correlated with enzymatic reaction-steps.
format	article
author	Wonseok Hwang Jungmin Yoo Yuno Lee Suyeon Park Phuong Lien Hoang HyeokJin Cho Jeongmin Yu Thi Minh Hoa Vo Minsang Shin Mi Sun Jin Daeho Park Changbong Hyeon Gwangrog Lee
author_facet	Wonseok Hwang Jungmin Yoo Yuno Lee Suyeon Park Phuong Lien Hoang HyeokJin Cho Jeongmin Yu Thi Minh Hoa Vo Minsang Shin Mi Sun Jin Daeho Park Changbong Hyeon Gwangrog Lee
author_sort	Wonseok Hwang
title	Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
title_short	Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
title_full	Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
title_fullStr	Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
title_full_unstemmed	Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
title_sort	dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/2a0749b91eb14221840220e83adac0c3
work_keys_str_mv	AT wonseokhwang dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT jungminyoo dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT yunolee dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT suyeonpark dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT phuonglienhoang dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT hyeokjincho dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT jeongminyu dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT thiminhhoavo dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT minsangshin dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT misunjin dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT daehopark dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT changbonghyeon dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis AT gwangroglee dynamiccoordinationoftwometalionsorchestrateslexonucleasecatalysis
_version_	1718383145697411072

Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis

Ejemplares similares