Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.

<h4>Background</h4>Primary defects in host immune responses have been hypothesised to contribute towards an inability of subjects with cystic fibrosis (CF) to effectively clear pulmonary infections. Innate T-lymphocytes provide rapid pathogen-specific responses prior to the development o...

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Autores principales:	Daniel J Smith, Geoffrey R Hill, Scott C Bell, David W Reid
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a087e3964c043a8ac54a64c5d843b3e
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spelling	oai:doaj.org-article:2a087e3964c043a8ac54a64c5d843b3e2021-11-25T05:57:29ZReduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.1932-620310.1371/journal.pone.0109891https://doaj.org/article/2a087e3964c043a8ac54a64c5d843b3e2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109891https://doaj.org/toc/1932-6203<h4>Background</h4>Primary defects in host immune responses have been hypothesised to contribute towards an inability of subjects with cystic fibrosis (CF) to effectively clear pulmonary infections. Innate T-lymphocytes provide rapid pathogen-specific responses prior to the development of classical MHC class I and II restricted T-cell responses and are essential to the initial control of pulmonary infection. We aimed to examine the relationship between peripheral blood lymphocyte phenotype and clinical outcomes in adults with CF.<h4>Methods</h4>We studied 41 subjects with CF and 22, age matched, non-smoking healthy control subjects. Lymphocytes were extracted from peripheral blood samples and phenotyped by flow-cytometry. Lymphocyte phenotype was correlated with sputum microbiology and clinical parameters.<h4>Results</h4>In comparison to healthy control subjects, mucosal associated invariant T (MAIT)-lymphocytes were significantly reduced in the peripheral blood of subjects with CF (1.1% versus 2.0% of T-lymphocytes, P = 0.002). MAIT cell concentration was lowest in CF subjects infected with P. aeruginosa and in subjects receiving treatment for a pulmonary exacerbation. Furthermore a reduced MAIT cell concentration correlated with severity of lung disease.<h4>Conclusion</h4>Reduced numbers of MAIT cells in subjects with CF were associated with P. aeruginosa pulmonary infection, pulmonary exacerbations and more severe lung disease. These findings provide the impetus for future studies examining the utility of MAIT cells in immunotherapies and vaccine development. Longitudinal studies of MAIT cells as biomarkers of CF pulmonary infection are awaited.Daniel J SmithGeoffrey R HillScott C BellDavid W ReidPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109891 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel J Smith Geoffrey R Hill Scott C Bell David W Reid Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.
description	<h4>Background</h4>Primary defects in host immune responses have been hypothesised to contribute towards an inability of subjects with cystic fibrosis (CF) to effectively clear pulmonary infections. Innate T-lymphocytes provide rapid pathogen-specific responses prior to the development of classical MHC class I and II restricted T-cell responses and are essential to the initial control of pulmonary infection. We aimed to examine the relationship between peripheral blood lymphocyte phenotype and clinical outcomes in adults with CF.<h4>Methods</h4>We studied 41 subjects with CF and 22, age matched, non-smoking healthy control subjects. Lymphocytes were extracted from peripheral blood samples and phenotyped by flow-cytometry. Lymphocyte phenotype was correlated with sputum microbiology and clinical parameters.<h4>Results</h4>In comparison to healthy control subjects, mucosal associated invariant T (MAIT)-lymphocytes were significantly reduced in the peripheral blood of subjects with CF (1.1% versus 2.0% of T-lymphocytes, P = 0.002). MAIT cell concentration was lowest in CF subjects infected with P. aeruginosa and in subjects receiving treatment for a pulmonary exacerbation. Furthermore a reduced MAIT cell concentration correlated with severity of lung disease.<h4>Conclusion</h4>Reduced numbers of MAIT cells in subjects with CF were associated with P. aeruginosa pulmonary infection, pulmonary exacerbations and more severe lung disease. These findings provide the impetus for future studies examining the utility of MAIT cells in immunotherapies and vaccine development. Longitudinal studies of MAIT cells as biomarkers of CF pulmonary infection are awaited.
format	article
author	Daniel J Smith Geoffrey R Hill Scott C Bell David W Reid
author_facet	Daniel J Smith Geoffrey R Hill Scott C Bell David W Reid
author_sort	Daniel J Smith
title	Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.
title_short	Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.
title_full	Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.
title_fullStr	Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.
title_full_unstemmed	Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.
title_sort	reduced mucosal associated invariant t-cells are associated with increased disease severity and pseudomonas aeruginosa infection in cystic fibrosis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/2a087e3964c043a8ac54a64c5d843b3e
work_keys_str_mv	AT danieljsmith reducedmucosalassociatedinvarianttcellsareassociatedwithincreaseddiseaseseverityandpseudomonasaeruginosainfectionincysticfibrosis AT geoffreyrhill reducedmucosalassociatedinvarianttcellsareassociatedwithincreaseddiseaseseverityandpseudomonasaeruginosainfectionincysticfibrosis AT scottcbell reducedmucosalassociatedinvarianttcellsareassociatedwithincreaseddiseaseseverityandpseudomonasaeruginosainfectionincysticfibrosis AT davidwreid reducedmucosalassociatedinvarianttcellsareassociatedwithincreaseddiseaseseverityandpseudomonasaeruginosainfectionincysticfibrosis
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Reduced mucosal associated invariant T-cells are associated with increased disease severity and Pseudomonas aeruginosa infection in cystic fibrosis.

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