Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal

Background. PCR assays have been developed for the diagnosis of dermatophytes, yet data in African populations are scarce. Objective. This study aimed to compare two PCR assays for the diagnosis of dermatophytosis in outpatients at the Aristide Le Dantec University Hospital in Dakar, Senegal. Patien...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jihane Kabtani, Khadim Diongue, Jean-Noël Dione, Anne Delmas, Coralie L’Ollivier, Marie-Claude Amoureux, Daouda Ndiaye, Stéphane Ranque
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dermatophytoses tinea capitis tinea unguium tinea pedis tinea corporis real-time PCR Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2a12e0a1e82e420f92bd20fdc8abd17c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a12e0a1e82e420f92bd20fdc8abd17c
record_format	dspace
spelling	oai:doaj.org-article:2a12e0a1e82e420f92bd20fdc8abd17c2021-11-25T18:06:04ZReal-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal10.3390/jof71109492309-608Xhttps://doaj.org/article/2a12e0a1e82e420f92bd20fdc8abd17c2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/949https://doaj.org/toc/2309-608XBackground. PCR assays have been developed for the diagnosis of dermatophytes, yet data in African populations are scarce. Objective. This study aimed to compare two PCR assays for the diagnosis of dermatophytosis in outpatients at the Aristide Le Dantec University Hospital in Dakar, Senegal. Patients and methods. A total of 105 samples, including 24 skin, 19 nail and 62 hair samples collected from 99 patients were included in this study. Each sample was subjected to conventional diagnosis (CD), including direct microscopy and culture, and two real-time PCR assays: one in-house (IH)-PCR, used at the University Hospital of Marseille and the Eurobio Scientific commercial kit (CK): designed for the specific detection of six dermatophytes not including <i>Microsporum audouinii</i>. Results. Of the 105 specimens, 24.8%, 36.2% and 20% were positive by CD, IH-PCR and CK-PCR, respectively. The IH-PCR and CK-PCR exhibited 88.9% and 65.4% sensitivity, respectively. With a 36.6 diagnostic odd ratio and 1.41 needed to diagnose, the IH-PCR displayed better diagnostic indices than the CK-PCR. It is notable that, when considering the species that it claims to detect, when it came to skin and nail samples, CK-PCR sensitivity increased to 77%. Conclusions. The pan-dermatophyte IH-PCR performed better in the diagnosis of dermatophytosis in this African population than the CK-PCR, which is not designed to detect <i>M. audouinii</i>. Nevertheless, both assays exhibited similarly good diagnostic indices for tinea corporis and tinea unguium, both of which are localisations where <i>M. audouinii</i> is more rarely involved than in tinea capitis.Jihane KabtaniKhadim DiongueJean-Noël DioneAnne DelmasCoralie L’OllivierMarie-Claude AmoureuxDaouda NdiayeStéphane RanqueMDPI AGarticledermatophytosestinea capitistinea unguiumtinea pedistinea corporisreal-time PCRBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 949, p 949 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dermatophytoses tinea capitis tinea unguium tinea pedis tinea corporis real-time PCR Biology (General) QH301-705.5
spellingShingle	dermatophytoses tinea capitis tinea unguium tinea pedis tinea corporis real-time PCR Biology (General) QH301-705.5 Jihane Kabtani Khadim Diongue Jean-Noël Dione Anne Delmas Coralie L’Ollivier Marie-Claude Amoureux Daouda Ndiaye Stéphane Ranque Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
description	Background. PCR assays have been developed for the diagnosis of dermatophytes, yet data in African populations are scarce. Objective. This study aimed to compare two PCR assays for the diagnosis of dermatophytosis in outpatients at the Aristide Le Dantec University Hospital in Dakar, Senegal. Patients and methods. A total of 105 samples, including 24 skin, 19 nail and 62 hair samples collected from 99 patients were included in this study. Each sample was subjected to conventional diagnosis (CD), including direct microscopy and culture, and two real-time PCR assays: one in-house (IH)-PCR, used at the University Hospital of Marseille and the Eurobio Scientific commercial kit (CK): designed for the specific detection of six dermatophytes not including <i>Microsporum audouinii</i>. Results. Of the 105 specimens, 24.8%, 36.2% and 20% were positive by CD, IH-PCR and CK-PCR, respectively. The IH-PCR and CK-PCR exhibited 88.9% and 65.4% sensitivity, respectively. With a 36.6 diagnostic odd ratio and 1.41 needed to diagnose, the IH-PCR displayed better diagnostic indices than the CK-PCR. It is notable that, when considering the species that it claims to detect, when it came to skin and nail samples, CK-PCR sensitivity increased to 77%. Conclusions. The pan-dermatophyte IH-PCR performed better in the diagnosis of dermatophytosis in this African population than the CK-PCR, which is not designed to detect <i>M. audouinii</i>. Nevertheless, both assays exhibited similarly good diagnostic indices for tinea corporis and tinea unguium, both of which are localisations where <i>M. audouinii</i> is more rarely involved than in tinea capitis.
format	article
author	Jihane Kabtani Khadim Diongue Jean-Noël Dione Anne Delmas Coralie L’Ollivier Marie-Claude Amoureux Daouda Ndiaye Stéphane Ranque
author_facet	Jihane Kabtani Khadim Diongue Jean-Noël Dione Anne Delmas Coralie L’Ollivier Marie-Claude Amoureux Daouda Ndiaye Stéphane Ranque
author_sort	Jihane Kabtani
title	Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
title_short	Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
title_full	Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
title_fullStr	Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
title_full_unstemmed	Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal
title_sort	real-time pcr assay for the detection of dermatophytes: comparison between an in-house method and a commercial kit for the diagnosis of dermatophytoses in patients from dakar, senegal
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2a12e0a1e82e420f92bd20fdc8abd17c
work_keys_str_mv	AT jihanekabtani realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT khadimdiongue realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT jeannoeldione realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT annedelmas realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT coralielollivier realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT marieclaudeamoureux realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT daoudandiaye realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal AT stephaneranque realtimepcrassayforthedetectionofdermatophytescomparisonbetweenaninhousemethodandacommercialkitforthediagnosisofdermatophytosesinpatientsfromdakarsenegal
_version_	1718411607387668480

Real-Time PCR Assay for the Detection of Dermatophytes: Comparison between an In-House Method and a Commercial Kit for the Diagnosis of Dermatophytoses in Patients from Dakar, Senegal

Ejemplares similares