Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen

ABSTRACT Antimicrobial resistance (AMR) is a major threat to global health, and it is crucial to understand the epidemiological aspects in order to predict the emergence and propagation of AMR genes. The aim of this study was to assess the variability and medium-term AMR trends within the mostly hea...

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Autores principales:	Christian Brinch, Pimlapas Leekitcharoenphon, Ana S. R. Duarte, Christina A. Svendsen, Jacob D. Jensen, Frank M. Aarestrup
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	metagenomics antimicrobial resistance microbiome sewage Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2a2522a867c74042ab3fdf63587418d8
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spelling	oai:doaj.org-article:2a2522a867c74042ab3fdf63587418d82021-12-02T19:47:33ZLong-Term Temporal Stability of the Resistome in Sewage from Copenhagen10.1128/mSystems.00841-202379-5077https://doaj.org/article/2a2522a867c74042ab3fdf63587418d82020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00841-20https://doaj.org/toc/2379-5077ABSTRACT Antimicrobial resistance (AMR) is a major threat to global health, and it is crucial to understand the epidemiological aspects in order to predict the emergence and propagation of AMR genes. The aim of this study was to assess the variability and medium-term AMR trends within the mostly healthy human population of a single city. We monitored over 36 months (November 2015 to November 2018) the AMR level in the city of Copenhagen, Denmark, by taking bi-weekly sewage samples from the inlets of the three main water treatment plants, extracting the DNA, performing metagenomic sequencing, and read-mapping against a database of known AMR genes. We found that the AMR level was surprisingly stable with no periodic variability and no signs of drift over the measured period. We found, however, that the seemingly random variations at each site correlate in time with each other, suggesting that the variations we see are due to real environmental changes in the occurrence of AMR. IMPORTANCE The Copenhagen sewage resistome is surprisingly stable in time. The implication is that, at least for cities that are comparable to Copenhagen in terms of sewer infrastructure, few or even single samples provide a robust picture of the resistome within a city.Christian BrinchPimlapas LeekitcharoenphonAna S. R. DuarteChristina A. SvendsenJacob D. JensenFrank M. AarestrupAmerican Society for Microbiologyarticlemetagenomicsantimicrobial resistancemicrobiomesewageMicrobiologyQR1-502ENmSystems, Vol 5, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	metagenomics antimicrobial resistance microbiome sewage Microbiology QR1-502
spellingShingle	metagenomics antimicrobial resistance microbiome sewage Microbiology QR1-502 Christian Brinch Pimlapas Leekitcharoenphon Ana S. R. Duarte Christina A. Svendsen Jacob D. Jensen Frank M. Aarestrup Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen
description	ABSTRACT Antimicrobial resistance (AMR) is a major threat to global health, and it is crucial to understand the epidemiological aspects in order to predict the emergence and propagation of AMR genes. The aim of this study was to assess the variability and medium-term AMR trends within the mostly healthy human population of a single city. We monitored over 36 months (November 2015 to November 2018) the AMR level in the city of Copenhagen, Denmark, by taking bi-weekly sewage samples from the inlets of the three main water treatment plants, extracting the DNA, performing metagenomic sequencing, and read-mapping against a database of known AMR genes. We found that the AMR level was surprisingly stable with no periodic variability and no signs of drift over the measured period. We found, however, that the seemingly random variations at each site correlate in time with each other, suggesting that the variations we see are due to real environmental changes in the occurrence of AMR. IMPORTANCE The Copenhagen sewage resistome is surprisingly stable in time. The implication is that, at least for cities that are comparable to Copenhagen in terms of sewer infrastructure, few or even single samples provide a robust picture of the resistome within a city.
format	article
author	Christian Brinch Pimlapas Leekitcharoenphon Ana S. R. Duarte Christina A. Svendsen Jacob D. Jensen Frank M. Aarestrup
author_facet	Christian Brinch Pimlapas Leekitcharoenphon Ana S. R. Duarte Christina A. Svendsen Jacob D. Jensen Frank M. Aarestrup
author_sort	Christian Brinch
title	Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen
title_short	Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen
title_full	Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen
title_fullStr	Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen
title_full_unstemmed	Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen
title_sort	long-term temporal stability of the resistome in sewage from copenhagen
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/2a2522a867c74042ab3fdf63587418d8
work_keys_str_mv	AT christianbrinch longtermtemporalstabilityoftheresistomeinsewagefromcopenhagen AT pimlapasleekitcharoenphon longtermtemporalstabilityoftheresistomeinsewagefromcopenhagen AT anasrduarte longtermtemporalstabilityoftheresistomeinsewagefromcopenhagen AT christinaasvendsen longtermtemporalstabilityoftheresistomeinsewagefromcopenhagen AT jacobdjensen longtermtemporalstabilityoftheresistomeinsewagefromcopenhagen AT frankmaarestrup longtermtemporalstabilityoftheresistomeinsewagefromcopenhagen
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Long-Term Temporal Stability of the Resistome in Sewage from Copenhagen

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