ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae

Rong Zhang,1–3 Ming Shao,1–3 Xu Han,1–3 Chuan Wang,3–4 Yong Li,3–4 Bin Hu,3–4 Daiwen Pang,3–4 Zhixiong Xie1–31Hubei Key Laboratory of Cell Homeostasis, 2College of Life Sciences, Wuhan University, 3Key Laboratory of Anal...

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Autores principales:	Zhang R, Shao M, Han X, Wang C, Li Y, Hu B, Pang DW, Xie ZX
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	ATP biosynthesis Saccharomyces cerevisiae QDs Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2a26260d1eef4babb216908ad04b9b5f
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spelling	oai:doaj.org-article:2a26260d1eef4babb216908ad04b9b5f2021-12-02T02:53:20ZATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae1178-2013https://doaj.org/article/2a26260d1eef4babb216908ad04b9b5f2017-05-01T00:00:00Zhttps://www.dovepress.com/atp-synthesis-in-the-energy-metabolism-pathway-a-new-perspective-for-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rong Zhang,1–3 Ming Shao,1–3 Xu Han,1–3 Chuan Wang,3–4 Yong Li,3–4 Bin Hu,3–4 Daiwen Pang,3–4 Zhixiong Xie1–31Hubei Key Laboratory of Cell Homeostasis, 2College of Life Sciences, Wuhan University, 3Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), 4College of Chemistry and Molecular Science, Wuhan University, Wuhan, People’s Republic of ChinaAbstract: Due to a growing trend in their biomedical application, biosynthesized nanomaterials are of great interest to researchers nowadays with their biocompatible, low-energy consumption, economic, and tunable characteristics. It is important to understand the mechanism of biosynthesis in order to achieve more efficient applications. Since there are only rare studies on the influences of cellular energy levels on biosynthesis, the influence of energy is often overlooked. Through determination of the intracellular ATP concentrations during the biosynthesis process, significant changes were observed. In addition, ATP synthesis deficiency caused great decreases in quantum dots (QDs) biosynthesis in the Δatp1, Δatp2, Δatp14, and Δatp17 strains. With inductively coupled plasma-atomic emission spectrometry and atomic absorption spectroscopy analyses, it was found that ATP affected the accumulation of the seleno-precursor and helped with the uptake of Cd and the formation of QDs. We successfully enhanced the fluorescence intensity 1.5 or 2 times through genetic modification to increase ATP or SeAM (the seleno analog of S-adenosylmethionine, the product that would accumulate when ATP is accrued). This work explains the mechanism for the correlation of the cellular energy level and QDs biosynthesis in living cells, demonstrates control of the biosynthesis using this mechanism, and thus provides a new manipulation strategy for the biosynthesis of other nanomaterials to widen their applications. Keywords: ATP, biosynthesis, Saccharomyces cerevisiae, QDs, CdSeZhang RShao MHan XWang CLi YHu BPang DWXie ZXDove Medical PressarticleATPbiosynthesisSaccharomyces cerevisiaeQDsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3865-3879 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	ATP biosynthesis Saccharomyces cerevisiae QDs Medicine (General) R5-920
spellingShingle	ATP biosynthesis Saccharomyces cerevisiae QDs Medicine (General) R5-920 Zhang R Shao M Han X Wang C Li Y Hu B Pang DW Xie ZX ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae
description	Rong Zhang,1–3 Ming Shao,1–3 Xu Han,1–3 Chuan Wang,3–4 Yong Li,3–4 Bin Hu,3–4 Daiwen Pang,3–4 Zhixiong Xie1–31Hubei Key Laboratory of Cell Homeostasis, 2College of Life Sciences, Wuhan University, 3Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), 4College of Chemistry and Molecular Science, Wuhan University, Wuhan, People’s Republic of ChinaAbstract: Due to a growing trend in their biomedical application, biosynthesized nanomaterials are of great interest to researchers nowadays with their biocompatible, low-energy consumption, economic, and tunable characteristics. It is important to understand the mechanism of biosynthesis in order to achieve more efficient applications. Since there are only rare studies on the influences of cellular energy levels on biosynthesis, the influence of energy is often overlooked. Through determination of the intracellular ATP concentrations during the biosynthesis process, significant changes were observed. In addition, ATP synthesis deficiency caused great decreases in quantum dots (QDs) biosynthesis in the Δatp1, Δatp2, Δatp14, and Δatp17 strains. With inductively coupled plasma-atomic emission spectrometry and atomic absorption spectroscopy analyses, it was found that ATP affected the accumulation of the seleno-precursor and helped with the uptake of Cd and the formation of QDs. We successfully enhanced the fluorescence intensity 1.5 or 2 times through genetic modification to increase ATP or SeAM (the seleno analog of S-adenosylmethionine, the product that would accumulate when ATP is accrued). This work explains the mechanism for the correlation of the cellular energy level and QDs biosynthesis in living cells, demonstrates control of the biosynthesis using this mechanism, and thus provides a new manipulation strategy for the biosynthesis of other nanomaterials to widen their applications. Keywords: ATP, biosynthesis, Saccharomyces cerevisiae, QDs, CdSe
format	article
author	Zhang R Shao M Han X Wang C Li Y Hu B Pang DW Xie ZX
author_facet	Zhang R Shao M Han X Wang C Li Y Hu B Pang DW Xie ZX
author_sort	Zhang R
title	ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae
title_short	ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae
title_full	ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae
title_fullStr	ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae
title_full_unstemmed	ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae
title_sort	atp synthesis in the energy metabolism pathway: a new perspective for manipulating cdse quantum dots biosynthesized in saccharomyces cerevisiae
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/2a26260d1eef4babb216908ad04b9b5f
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ATP synthesis in the energy metabolism pathway: a new perspective for manipulating CdSe quantum dots biosynthesized in Saccharomyces cerevisiae

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