Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity.
Bacillus anthracis produces a binary toxin composed of protective antigen (PA) and one of two subunits, lethal factor (LF) or edema factor (EF). Most studies have concentrated on induction of toxin-specific antibodies as the correlate of protective immunity, in contrast to which understanding of cel...
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2014
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oai:doaj.org-article:2a3497e4431447f1bf25d62970f3f8fb2021-11-18T06:06:37ZAnthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity.1553-73661553-737410.1371/journal.ppat.1004085https://doaj.org/article/2a3497e4431447f1bf25d62970f3f8fb2014-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24788397/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Bacillus anthracis produces a binary toxin composed of protective antigen (PA) and one of two subunits, lethal factor (LF) or edema factor (EF). Most studies have concentrated on induction of toxin-specific antibodies as the correlate of protective immunity, in contrast to which understanding of cellular immunity to these toxins and its impact on infection is limited. We characterized CD4+ T cell immunity to LF in a panel of humanized HLA-DR and DQ transgenic mice and in naturally exposed patients. As the variation in antigen presentation governed by HLA polymorphism has a major impact on protective immunity to specific epitopes, we examined relative binding affinities of LF peptides to purified HLA class II molecules, identifying those regions likely to be of broad applicability to human immune studies through their ability to bind multiple alleles. Transgenics differing only in their expression of human HLA class II alleles showed a marked hierarchy of immunity to LF. Immunogenicity in HLA transgenics was primarily restricted to epitopes from domains II and IV of LF and promiscuous, dominant epitopes, common to all HLA types, were identified in domain II. The relevance of this model was further demonstrated by the fact that a number of the immunodominant epitopes identified in mice were recognized by T cells from humans previously infected with cutaneous anthrax and from vaccinated individuals. The ability of the identified epitopes to confer protective immunity was demonstrated by lethal anthrax challenge of HLA transgenic mice immunized with a peptide subunit vaccine comprising the immunodominant epitopes that we identified.Stephanie AscoughRebecca J IngramKaren K ChuCatherine J ReynoldsJulie A MussonMehmet DoganayGökhan MetanYusuf OzkulLes BaillieShiranee SriskandanStephen J MooreTheresa B GallagherHugh DysonE Diane WilliamsonJohn H RobinsonBernard MaillereRosemary J BoytonDaniel M AltmannPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 5, p e1004085 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Stephanie Ascough Rebecca J Ingram Karen K Chu Catherine J Reynolds Julie A Musson Mehmet Doganay Gökhan Metan Yusuf Ozkul Les Baillie Shiranee Sriskandan Stephen J Moore Theresa B Gallagher Hugh Dyson E Diane Williamson John H Robinson Bernard Maillere Rosemary J Boyton Daniel M Altmann Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity. |
| description |
Bacillus anthracis produces a binary toxin composed of protective antigen (PA) and one of two subunits, lethal factor (LF) or edema factor (EF). Most studies have concentrated on induction of toxin-specific antibodies as the correlate of protective immunity, in contrast to which understanding of cellular immunity to these toxins and its impact on infection is limited. We characterized CD4+ T cell immunity to LF in a panel of humanized HLA-DR and DQ transgenic mice and in naturally exposed patients. As the variation in antigen presentation governed by HLA polymorphism has a major impact on protective immunity to specific epitopes, we examined relative binding affinities of LF peptides to purified HLA class II molecules, identifying those regions likely to be of broad applicability to human immune studies through their ability to bind multiple alleles. Transgenics differing only in their expression of human HLA class II alleles showed a marked hierarchy of immunity to LF. Immunogenicity in HLA transgenics was primarily restricted to epitopes from domains II and IV of LF and promiscuous, dominant epitopes, common to all HLA types, were identified in domain II. The relevance of this model was further demonstrated by the fact that a number of the immunodominant epitopes identified in mice were recognized by T cells from humans previously infected with cutaneous anthrax and from vaccinated individuals. The ability of the identified epitopes to confer protective immunity was demonstrated by lethal anthrax challenge of HLA transgenic mice immunized with a peptide subunit vaccine comprising the immunodominant epitopes that we identified. |
| format |
article |
| author |
Stephanie Ascough Rebecca J Ingram Karen K Chu Catherine J Reynolds Julie A Musson Mehmet Doganay Gökhan Metan Yusuf Ozkul Les Baillie Shiranee Sriskandan Stephen J Moore Theresa B Gallagher Hugh Dyson E Diane Williamson John H Robinson Bernard Maillere Rosemary J Boyton Daniel M Altmann |
| author_facet |
Stephanie Ascough Rebecca J Ingram Karen K Chu Catherine J Reynolds Julie A Musson Mehmet Doganay Gökhan Metan Yusuf Ozkul Les Baillie Shiranee Sriskandan Stephen J Moore Theresa B Gallagher Hugh Dyson E Diane Williamson John H Robinson Bernard Maillere Rosemary J Boyton Daniel M Altmann |
| author_sort |
Stephanie Ascough |
| title |
Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity. |
| title_short |
Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity. |
| title_full |
Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity. |
| title_fullStr |
Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity. |
| title_full_unstemmed |
Anthrax lethal factor as an immune target in humans and transgenic mice and the impact of HLA polymorphism on CD4+ T cell immunity. |
| title_sort |
anthrax lethal factor as an immune target in humans and transgenic mice and the impact of hla polymorphism on cd4+ t cell immunity. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/2a3497e4431447f1bf25d62970f3f8fb |
| work_keys_str_mv |
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